Development of HPLC fingerprint for the quality control ofEuonymus fortuneiand distinguishing it from related species

Abstract

Summary.To control the quality of Euonymus fortunei (Turcz.) Hand.-Mazz., a simple and reliable method of high-performance liquid chromatography (HPLC) coupled with photodiode array detector (PAD) was developed for both fingerprint analysis and quantitative determination. Four representative flavonoids, namely, kaempferolhamnopyranoside (IV) isolated from E. fortunei, were used as reference compounds and simultaneously determined by the validated HPLC method. The unique properties of the chromatographic fingerprint were validated by analyzing 11 batches of E. fortunei, E. japonicus, E. laxiflorus, E. myrianthus, and E. hamiltonianus samples. Our results revealed that the chromatographic fingerprint combined with similarity measurement could efficiently identify and distinguish E. fortunei from the other investigated Euonymus species.

Evaluations of Hemostatic Activity of Ethanol Extract of Euonymus fortunei Aerial Parts

“…Meanwhile, the n-butanol fraction exhibits stronger hemostatic activity than the total fraction, indicating that the n-butanol fraction may be the best hemostatic part, and its hemostatic effect may be related to platelet aggregation and clotting factors. According to our previous research, flavonoids such as kaempferol-3,7dirhamnopyranoside and kaempferol-3-(4″-Oacetyl)-O-α-L-rhamnopyranoside-7-O-α-Lrhamnopyranoside, are the main components in the n-butanol fraction of E. fortunei [8,20]. In addition to usually regarded as polyphenolic compounds with antioxidant activity, kaempferol-3,7-dirhamnopyranoside can also have a hypoglycemic effect [21], and may also be used for cardiovascular treat cardiovascular disorders, exerts endothelium-dependent and independent vasorelaxation in thoracic aorta of normotensive and hypertensive rats [22].…”

Total Phenolic, Total Flavonoid and Antioxidant Activity of Euonymus fortunei by HPLC-Based Analysis

“…HPLC analysis: Optimizations of sample extraction and chromatography conditions of HPLC analysis were carried out as reported in previous research 5 .…”

“…In our previous work, six flavonoid glycosides were isolated from the ethanol extract of E. fortunei 4 . Two compounds, namely kaempferol-3,7-O-α-dirhamnopyranoside and kaempferol-3-(4"-O-acetyl)-O-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside, were proved as the major class of constituents, with high contents of 0.03-0.54 and 0.12-0.67 mg/g, respectively 5 . Phenolic compounds are known to act as antioxidants and flavonoids are one of the most important phenolic compounds with radical scavenging properties 6 .…”