Evaluation of Antibiotic Susceptibility of Gram-Positive Anaerobic Cocci Isolated from Cancer Patients of the N. N. Blokhin Russian Cancer Research Center

Shilnikova, I. I.; Дмитриева, Н. В.

doi:10.1155/2015/648134

Cited by 22 publications

(20 citation statements)

References 16 publications

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“…Antibiotic susceptibility testing showed that all strains were sensitive to benzylpenicillin, amoxicillin, clindamycin, metronidazole, and vancomycin, according to the specific clinical breakpoints for anaerobic Gram-positive cocci set by EUCAST. This confirms previous studies (13,14) demonstrating an overall sensitivity to many of the antibiotics used to treat anaerobic infections. For two other antibiotics, moxifloxacin and linezolid, we had to apply non-species-specific breakpoints, since specific breakpoints were lacking.…”

Section: Discussionsupporting

confidence: 90%

“…Three of the isolates were classified as resistant to moxifloxacin and one isolate as resistant to linezolid. A previous study has also reported resistance to various fluoroquinolones for F. magna (14). No clinical breakpoints are available for rifampin, but one isolate displayed high MIC values (Ͼ32 mg/ml) for both rifampin and moxifloxacin.…”

Section: Discussionmentioning

confidence: 85%

“…The clinical presentation of bone and joint infections involving F. magna is represented both by early postoperative infections following prosthetic joint surgery accompanied by evident symptoms, and late infections often with mild and insidious symptoms (4)(5)(6). F. magna isolates are generally sensitive to the antibiotics routinely used to treat infections caused by anaerobic bacteria, but resistance to various antimicrobial agents, such as ␤-lactam antibiotics, clindamycin, fluoroquinolones, and metronidazole, has been reported (13,14).…”

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confidence: 99%

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Finegoldia magna Isolated from Orthopedic Joint Implant-Associated Infections

et al. 2017

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The anaerobic Gram-positive coccus is a rare cause of infections of bone and joints. The aim of this study was to describe the microbiological and clinical characteristics of orthopedic implant-associated infections caused by We retrospectively analyzed samples consisting of anaerobic Gram-positive cocci and samples already identified as from patients with orthopedic infections. The isolates found were determined to the species level using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The antibiotic susceptibility pattern was determined by Etest. Whole-genome sequencing (WGS) was performed. Clinical data were extracted from each patient's journal. In nine patients, orthopedic joint implant-associated infections were identified as being caused by The isolates were susceptible to most of the antibiotics tested, with the exception of rifampin and moxifloxacin in a few cases. Five of the nine infections were monomicrobial. The most common antibiotic used to treat the infection was penicillin V, but five of the nine patients received a combination of antibiotics. Eight patients underwent surgical treatment, with extraction of the implant performed in seven cases and reimplantation in only two cases. The WGS showed a relatively small core genome, with 126,647 single nucleotide polymorphisms identified within the core genome. A phylogenomic analysis revealed that the isolates clustered into two distinct clades. Orthopedic implant-associated infections caused by are rare, but the bacteria are generally susceptible to antibiotics. Despite this, surgical treatment combined with long-term antibiotics is often necessary. The WGS analysis revealed a high heterogeneity and suggested the existence of at least two different species.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 85%

mentioning

confidence: 99%

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Finegoldia magna Isolated from Orthopedic Joint Implant-Associated Infections

et al. 2017

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“…Other mechanisms of reduced susceptibility include lower levels of the PFOR enzyme (a significant decrease of the reducing power of the microorganisms) and the induction of lactate dehydrogenase (LDH) activity. These resistance determinants are predominantly found in the chromosome but their presence has been described in transferable plasmids and IS elements as well, with some experts fearing the rapid dissemination of these genes [ 149 , 150 , 185 , 186 , 187 , 188 , 189 , 190 , 191 , 192 , 193 , 194 ].…”

Section: Antibiotic Resistance In Anaerobic Bacteria: the Importanmentioning

confidence: 99%

Identification and Antimicrobial Susceptibility Testing of Anaerobic Bacteria: Rubik’s Cube of Clinical Microbiology?

2017

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Anaerobic bacteria have pivotal roles in the microbiota of humans and they are significant infectious agents involved in many pathological processes, both in immunocompetent and immunocompromised individuals. Their isolation, cultivation and correct identification differs significantly from the workup of aerobic species, although the use of new technologies (e.g., matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, whole genome sequencing) changed anaerobic diagnostics dramatically. In the past, antimicrobial susceptibility of these microorganisms showed predictable patterns and empirical therapy could be safely administered but recently a steady and clear increase in the resistance for several important drugs (β-lactams, clindamycin) has been observed worldwide. For this reason, antimicrobial susceptibility testing of anaerobic isolates for surveillance purposes or otherwise is of paramount importance but the availability of these testing methods is usually limited. In this present review, our aim was to give an overview of the methods currently available for the identification (using phenotypic characteristics, biochemical testing, gas-liquid chromatography, MALDI-TOF MS and WGS) and antimicrobial susceptibility testing (agar dilution, broth microdilution, disk diffusion, gradient tests, automated systems, phenotypic and molecular resistance detection techniques) of anaerobes, when should these methods be used and what are the recent developments in resistance patterns of anaerobic bacteria.

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“…11 However, three strains were described as highly resistant to metronidazole. 11,12 All serotypes of S pneumoniae strains carry a common C-polysaccharide found in the cell wall and, detected by PATB, our results indicate that P micra probably harbors a similar polysaccharide. This polysaccharide does not seem to be shared with different species of Peptostreptococcus because no cross-reactivity has been observed by the manufacturer with the Peptostreptococcus anaerobius reference strain.…”

Section: Discussionmentioning

confidence: 54%