Evaluation of antibiotic resistance patterns and heavy metals tolerance of some bacteria isolated from contaminated soils and sediments from Warri, Delta State, Nigeria

Abstract

This work investigated the antibiotic resistance patterns and heavy metals such as Lead (Pb), Zinc (Zn), Cadmium (Cd) and iron (Fe) tolerance of selected bacteria isolated from contaminated soils and sediments around Warri area of Delta State. The heterotrophic bacterial counts for the sampled soils and sediments ranged from 1.7×105 cfu/g to 5.7×105cfu/g for Ubeji river sediments, 1.0×105 cfu/g to 9.0×105cfu/g for spare parts dumpsite and 1.2×104cfu/g to 9.0×104 cfu/g for Ifie depot sites respectively. The characterized bacterial isolates included; Klebsiella sp, Bacillus subtilis, Streptococcus sp., Escherichia coli, Klebsiella mobilis and Staphylococcus sp., Micrococcus sp. and Pseudomonas aeroginosa. Bacterial isolates showed multiple drug resistance and the most resistant isolates were S. aureus, E. coli and P. aeroginosa while K. mobilis exhibited the least resistance. The tolerance of the bacterial isolates exposed to varying concentrations of Pb2+, Cd2+, Fe2+, and Zn2+was ascertained using agar diffusion method. All the bacterial isolates exhibited varying degree of susceptibility at different concentrations of Pb and Cd while the organisms displayed abundant and moderate growth in the presence of Fe and Zn even at higher concentrations. The ability of these bacteria to resist antibiotics and heavy metal tolerance could present serious danger to the environment as the resistance genes may be transferred to surrounding wild type microbial cells.Keywords: Heavy metals, Antibiotics resistance, tolerance, Ubeji river sediments, Warri

Effect of Bio-enhanced Streptococcus pyogenes and Enterococcus faecalis Co-culture on Decontamination of Heavy Metals Content in Used Lubricating Oil Contaminated Soil

“…However, at the tenth week, PCD only enhanced complete removal of Cd on all used lubricating oil contamination levels compared with compost and PCPH only that attained complete removal of Cd on 5% and 15% used lubricating oil contamination levels, respectively (Table 4). In line with the results obtained in this study, Eghomwanre et al (2016)…”

Heavy Metal Tolerance of Bacterial Isolates from Solid Waste Dumping Sites in Abuja, Nigeria

“…Also, Bacillus and Pseudomonas spp., which can tolerate Cr, Pb, Fe, Hg, and Cu had been documented in previous works (Hassen et al, 1998;Nath et al, 2019;Imron et al, 2021). The mechanisms used by microorganisms to resist/tolerance heavy metals have been linked to modification of their cell membrane regulation, complexation/stabilization, enzymatic transformation/detoxification and plasmid-mediation (Ianeva, 2009;Sevgi et al, 2010;Eghomwanre et al, 2016). The use of extracellular polysaccharides, extracellular and intracellular sequestration by Pseudomonas spp.…”

“…which displayed least). The variations in the MICs could be attributed to the differences in their cell wall components and abilities to synthesize proteins with special abilities to bind metals and regulate their effects (Sevgi et al, 2010;Eghomwanre et al 2016;Mosa et al, 2016). A comparison of the four remarkable isolates (Proteus, Providencia, Pseudomonas and Bacillus) revealed that Bacillus spp.…”

Heavy metal co-resistance with antibiotics amongst bacteria isolates from an open dumpsite soil

“…In an earlier study, S. aureus, E. coli and P. aeruginosa were reported as the most resistant isolates to antibiotics and these isolates apart from S. aureus showed MDR in our study. Furthermore, these MDR in their study showed abundant to moderate growth with iron and zinc at higher concentrations [ 39 ]. Compared to our findings, B. cereus, B. subtilis, and E. coli all showed complete resistance to Pb.…”

“…Isolates showing multiple drug resistance (resistance to atleast 2 antibiotics) were selected for heavy metal resistance test. This was done using as previously reported [ 26 , 39 ]. Briefly, a loopful of 12–16 h old bacteria culture in tryptic soy broth was inoculated on Mueller- Hinton Agar plates bearing different concentrations (50, 100, 150, 200 and 250 μg/ml) of the test heavy metal (chromium, vanadium, cobalt, cadmium and lead).…”