Evaluation of antibacterial and antioxidant activity of extracts of endophytic fungi isolated from Indonesian Zingiberaceous plants

Abstract

Abstract. Praptiwi, Palupi KD, Fathoni A, Wulansari D, Ilyas M, Agusta A. 2016. Evaluation of antibacterial and antioxidant activity of extracts of endophytic fungi isolated from Indonesian Zingiberaceous plants. . Thirty two endophytic fungi isolated from rhizome of six species of Zingiberaceae from Karanganyar, Central Java, Indonesia. Based on their morphological characters, all of endophytic isolates were classified into 10 genera. All of endophytic fungi isolates were then cultivated into 200 mL of Potato Dextrose Broth and incubated at room temperature. After three weeks, each of the fungi culture were extracted with appropriate amount of ethyl acetate, and dried under reduced pressure. The extracts, then evaluated for DPPH antioxidant activity and antibacterial activities against Escherichia coli Ina-CC B5 and Staphylococcus aureus Ina-CC B4 based on non-eluted TLC bioautography assays. The results showed there are 19 extracts possessing antioxidant activity, 23 extracts inhibit the growth of E. coli Ina-CC B5, and 19 extracts inhibit the growth of S. aureus Ina-CC B4. The minimum inhibitory concentration (MIC) value of active extracts against S. aureus were in the range of 80-1280 ug/mL, while MIC against E. coli were in the range of 80->1280 ug/mL. The IC 50 of active extracts were in the range of 50.97-2031.26 ppm. Further analysis by the eluted TLC-bioautography assay of active extracts showed that several fungal metabolites on active extract responsible for its/their bioactivity. It can be concluded that endophytic fungi isolated from Zingiberaceae can be used as new antibacterial and antioxidant resources.

Evaluation of Antibacterial Activity, Total Phenolic and Flavonoid Contents of Extracts of Endophytic Fungi Associated with Tinospora crispa (L.) Hook. f. & Thomson

“…In other studies, endophytic fungi Phomopsis genera produced potent antibacterial compounds such as against Pseudomonas syringae and were reported as the source of bioactive metabolites with various biological action [33], [34]. While endophytic fungi Dematiaceae family was reported to be excellent antibacterial against S. aureus bacteria [35]. The endophytic fungi Dematiaceae family, such as Alternaria, also exhibited various biological activities such as antinematode [36], cytotoxic [36][37] [38], antimicrobial [36], [37], anti-viral [39], anti-parasitic [40], enzyme inhibitors [38], [41], [42].…”

Evaluation of Antibacterial and Antioxidant Activities of Stingless Bee (Tetragonula Laeviceps) Hive from Purwodadi Botanical Garden and LC/MS Profiles of Dichloromethane Fraction

“…After completion of dilution, 100 µL of bacterial suspension was added to each well (5 x 105 CFU/mL) and then incubated at 37 °C in humid conditions for 18-20 h. After incubation, 10 µL of iodonitrotetrazolium chloride (INT) was added to each well and incubated for 15-30 min. The wells that did not change color to red indicated the presence of antibacterial activity [20].…”

“…coli (lower, right). Note: hexane [17], dichloromethane [18], ethyl acetate [19], methanol [20], acetone-water [21]. Upper, left: observed under 366 nm, Lower, left: observed under 254 nm.…”

Familiar Edible Flowers in Indonesia

“…This flower is in the form of this shrub tree, spread in tropical countries of Asia and Africa. Soka flowers are used as ornamental plants and also traditionally to treat diarrhea, fever, headaches, wound medicine, and stomach ulcers 22 .…”