Evaluation of Anti-proliferative Potential and Antioxidant Activity of a Wild Edible Mushroom Macrocybe crassa (Sacc.) Pegler and Lodge

Abstract

Objective: This study aims to quantify the anti-oxidant activity of the methanolic extract of Macrocybe crassa and its anti-proliferative activity on normal and cancer cells. Methods: The anti-oxidant potential of the extract was determined by several in vitro assay system like DPPH radical scavenging activity, superoxide anion scavenging activity, percentage inhibition of lipid peroxidation and nitric oxide (NO) scavenging activity. Anti-proliferative activity was tested by MTT assay on breast cancer cell line MCF7, Human acute T lymphoblastic leukaemic cell MOLT-4 and Peripheral Blood Mononuclear Cells or PBMC isolated from a healthy donor to check its cytotoxic effect on normal cells. Results: Results indicated that the methanolic extract of Macrocybe crassa shows appreciable anti-proliferative activity against breast cancer cell line MCF7 and negligible effect on MOLT4 cells. In contrast no significant anti-proliferative effect has been observed in normal PMMCs. Moderate anti-oxidant activity was recorded in methanolic extract. Conclusion: Methanolic extract of of Macrocybe crassa with moderate anti-oxidant activity and specific anti-proliferative effect on MCF7 holds a great promise can be used for isolation of bio molecules for treating Breast Cancer.

Assessment of the Impact of Wild Stinkhorn Mushroom Extracts on Different Cancer Cell Proliferation and Study of Primary Metabolites

“…The percentage of scavenging of DPPH radical increase 10.073±0.8250 % to 61.276±8.286% at concentration range 100 µg/mL to 500 µg/mL (Table 4). The IC 50 value is the particular concentration at which 50% of free radical is scavenged by antioxidant molecule of the extract 5 . The IC 50 value of MEP against the DPPH + free radical was 426.723±17.574 µg/mL (Table 5).…”

“…Cancer is characterized by uncontrolled cell proliferation and it may appear in different forms such as lung, breast, colon, skin, blood cancer etc. 5 . In India, it is now cause of catastrophic health spending, calamity financing and rising expenditure before death [4][5][6][7][8] .…”

“…In India, it is now cause of catastrophic health spending, calamity financing and rising expenditure before death [4][5][6][7][8] . The invasiveness and mortality rate of the cancer vary among different forms, lung cancer is highly fatal with a very high death rate of 70%, followed by colon cancer and breast cancer 5 .The most commonly used potential strategy for treating cancer is the use of various chemotherapeutic drugs along with radiotherapy 9 . Chemotherapeutic drug should be target specific to cancer cells, and should not affect the normal cells.…”