Evaluation of an electrochemical biosensor based on carbon nanotubes, hydroxyapatite and horseradish peroxidase for the detection of hydrogen peroxide

Alvarez-Paguay, Jocelyne; Fernández, Lenys; Bolaños-Méndez, Diego; González, Gema; Espinoza-Montero, Patricio J.

doi:10.1016/j.sbsr.2022.100514

Cited by 6 publications

(7 citation statements)

References 70 publications

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“…The developed sensor showed excellent electrochemical oxidation of glucose with a LOD of 0.2 µM in phosphate buffer (pH 6.5). Alvarez-Paguay and colleagues developed an H 2 O 2 biosensor using HRP and hydroxyapatite-functionalized CNTs (HAp-CNTs) modified GCE [ 70 ]. HAp-CNT protects the bioactivity and improves the conductivity of immobilized HRP.…”

Section: Various Nanomaterial-modified Electrodesmentioning

confidence: 99%

Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules

Theyagarajan

Kim

2023

Biosensors

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Electrochemical biosensors are superior technologies that are used to detect or sense biologically and environmentally significant analytes in a laboratory environment, or even in the form of portable handheld or wearable electronics. Recently, imprinted and implantable biosensors are emerging as point-of-care devices, which monitor the target analytes in a continuous environment and alert the intended users to anomalies. The stability and performance of the developed biosensor depend on the nature and properties of the electrode material or the platform on which the biosensor is constructed. Therefore, the biosensor platform plays an integral role in the effectiveness of the developed biosensor. Enormous effort has been dedicated to the rational design of the electrode material and to fabrication strategies for improving the performance of developed biosensors. Every year, in the search for multifarious electrode materials, thousands of new biosensor platforms are reported. Moreover, in order to construct an effectual biosensor, the researcher should familiarize themself with the sensible strategies behind electrode fabrication. Thus, we intend to shed light on various strategies and methodologies utilized in the design and fabrication of electrochemical biosensors that facilitate sensitive and selective detection of significant analytes. Furthermore, this review highlights the advantages of various electrode materials and the correlation between immobilized biomolecules and modified surfaces.

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Section: Various Nanomaterial-modified Electrodesmentioning

confidence: 99%

Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules

Theyagarajan

Kim

2023

Biosensors

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“…Based on the type of biorecognition elements, different methods can be adopted to attach them to the electrode For streptavidin functionalization, 130 utilized for enzyme immobilization 119 enhanced sensitivity, detection range, lowered detection limit and response time 119 Composite: HAp-CNT 120 CNT enhances electrical communication, HAp was used for immobilizing HRP 120…”

Section: Fabrication Of Bioelectrodesmentioning

confidence: 99%

“…The COF shell in such a microcapsule protects the enzymes from harsh external environments. Carbon-based materials , and nanomaterials such as graphene and its derivatives, , carbon nanotubes , are also widely used as a platform for immobilizing the biorecognition element and for improving the biosensor performance. Similarly, noble metal nanoparticles (NPs), such as gold, silver, palladium and platinum NPs, metal nanoclusters, metal oxides and sulfides have been extensively used for the fabrication of bioelectrode interfaces. ,,, MXenes are relatively a new class of material and possesses properties like large surface area, high electrical conductivity, hydrophilicity, biocompatibility and ease of functionalization which make these materials suitable for biosensor electrode fabrication .…”

Section: Materials For Bioelectrode Fabricationmentioning

confidence: 99%

“…114 The COF shell in such a microcapsule protects the enzymes from harsh external environments. Carbon-based materials 115,116 and nanomaterials such as graphene and its derivatives, 117,118 carbon nanotubes 119,120 are also widely used as a platform for immobilizing the biorecognition element and for improving the biosensor performance. Similarly, noble metal nanoparticles (NPs), such as gold, silver, palladium and platinum NPs, metal nanoclusters, metal oxides and sulfides have been extensively used for the fabrication of bioelectrode interfaces.…”

Section: Materials For Bioelectrode Fabricationmentioning

confidence: 99%

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Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Kalita,

Gogoi,

Minteer

et al. 2023

ACS Meas. Sci. Au

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The critical performance factors such as selectivity, sensitivity, operational and storage stability, and response time of electrochemical biosensors are governed mainly by the function of their key component, the bioelectrode. Suitable design and fabrication strategies of the bioelectrode interface are essential for realizing the requisite performance of the biosensors for their practical utility. A multifaceted attempt to achieve this goal is visible from the vast literature exploring effective strategies for preparing, immobilizing, and stabilizing biorecognition elements on the electrode surface and efficient transduction of biochemical signals into electrical ones (i.e., current, voltage, and impedance) through the bioelectrode interface with the aid of advanced materials and techniques. The commercial success of biosensors in modern society is also increasingly influenced by their size (and hence portability), multiplexing capability, and coupling in the interface of the wireless communication technology, which facilitates quick data transfer and linked decision-making processes in real-time in different areas such as healthcare, agriculture, food, and environmental applications. Therefore, fabrication of the bioelectrode involves careful selection and control of several parameters, including biorecognition elements, electrode materials, shape and size of the electrode, detection principles, and various fabrication strategies, including microscale and printing technologies. This review discusses recent trends in bioelectrode designs and fabrications for developing electrochemical biosensors. The discussions have been delineated into the types of biorecognition elements and their immobilization strategies, signal transduction approaches, commonly used advanced materials for electrode fabrication and techniques for fabricating the bioelectrodes, and device integration with modern electronic communication technology for developing electrochemical biosensors of commercial interest.

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“…Hydroxyapatite (HAP), the major biomineral of bones and teeth, stably exists in various parts of the human body, owing to its high stability and excellent biocompatibility. It is widely used in tissue repair, biomimetic materials, drugs/gene delivery, , electrochemical sensing, − and so on. HAP has also been frequently applied for NA separation since Bernardi found its excellent discriminating capacity for single- and double-stranded DNA .…”

Section: Introductionmentioning

confidence: 99%

Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Chen

Yang

et al. 2023

ACS Appl. Nano Mater.

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The unique discriminating ability of hydroxyapatite (HAP) to single- and double-stranded nucleic acid implies its potential as a biosensing material for nucleic acid analysis. Herein, a novel immobilization- and label-free electrochemical strategy has been developed for miRNA let-7a detection based on the specific adsorption of magnetic Fe3O4@hydroxyapatite (Fe3O4@HAP) to a duplex structure. Typically, the highly efficient homogeneous reaction between probe DNA (pDNA) and target miRNA let-7a produces the nucleic acid duplex, which is specifically adsorbed on the surface of Fe3O4@HAP. Thereafter, the electroactive intercalator Nile blue (NB) is introduced as the label-free signal molecule and inserts between the stacked base pairs of the pDNA–miRNA duplex. Thus, the Fe3O4@HAP bearing pDNA–miRNA and numerous intercalated NBs are adsorbed by the magnetic glassy carbon electrode, giving rise to an intense sensing signal without the need of nuclease-assisted cyclic amplification. With this advantage, the sensing system shows a wide linear range from 1.0 fM to 100 nM and a low detection limit of 0.051 fM for miRNA let-7a analysis. What is more, the synergy of the base-pairing-driven hybridization, the specific intercalation of NB to the duplex structure, and the selective adsorption of Fe3O4@HAP contribute to a high sensing specificity of the biosensing strategy. The sensing method provides a state-of-the-art strategy for the sensitive and facile bioanalysis and clinical diagnosis of miRNA.

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Evaluation of an electrochemical biosensor based on carbon nanotubes, hydroxyapatite and horseradish peroxidase for the detection of hydrogen peroxide

Cited by 6 publications

References 70 publications

Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules

Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

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Evaluation of an electrochemical biosensor based on carbon nanotubes, hydroxyapatite and horseradish peroxidase for the detection of hydrogen peroxide

Cited by 6 publications

References 70 publications

Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules

Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Specific Adsorption of Magnetic Fe3O4@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

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Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA