Evaluation of an atrophic rhinitis vaccine under controlled conditions

Mt, Voets; Klaassen, C. H. L.; Charlier, P; Wiseman, A; Descamps, J.

doi:10.1136/vr.130.25.549

Cited by 4 publications

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“…The proportion was 5 times (nasal swabs) and 3 times (tonsils) higher than in piglets from a study performed with a vaccine containing Bb/Pm/fPMT adjuvanted with oil in water in the Netherlands (Voets et al, 1992). This may be due to differences in the bacterial vaccine components, the adjuvant or the challenge strains.…”

Section: Acta Veterinaria Hungarica 56 2008mentioning

confidence: 72%

Atrophic rhinitis vaccine composition triggers different serological profiles that do not correlate with protection

Magyar

Donkó

Kovács

2008

Acta Veterinaria Hungarica

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Atrophic rhinitis (AR) is a widespread and economically important disease of swine caused by Bordetella bronchiseptica and Pasteurella multocida. It can be controlled by vaccination. This study investigates the effect of altering the composition (adjuvants and/or addition of formalin-inactivated P. multocida toxin, fPMT) of conventional vaccines on the serological profile and on protection against AR in swine. A significantly higher B. bronchiseptica specific antibody titre was detected for vaccines with novel immunostimulants, the best being Montanide IMS 1313 (1:630 compared to 1:274 obtained with alum). The highest B. bronchiseptica antibody titre was demonstrated for a combination of B. bronchiseptica -fPMT, while PMT antibody titre was highest for monovalent fPMT (both adjuvanted with IMS 1313). The AR-specific antibodies were transmitted from dams to their offspring in similar titres and with the same hierarchy of effectiveness. After a B. bronchiseptica -P. multocida bacterial challenge, piglets from dams vaccinated with fPMT combined with B. bronchiseptica or B. bronchiseptica -P. multocida bacterins showed the lowest nasal lesions scores (4.5 and 3.2, respectively, out of a possible maximum score of 18). These combinations, both of which were adjuvanted with IMS 1313, gave the best protection against experimentally induced AR. Our results show that the adjuvant and the antigen composition of the vaccine strongly affect seroconversion, and that the AR-specific antibody titre does not necessarily correlate with the degree of protection.

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Section: Acta Veterinaria Hungarica 56 2008mentioning

confidence: 72%

Atrophic rhinitis vaccine composition triggers different serological profiles that do not correlate with protection

Magyar

Donkó

Kovács

2008

Acta Veterinaria Hungarica

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“…Previous experiments have shown that the dermonecrotic toxin (DNT) of B. bronchiseptica and P. multocida are virulence factors producing AR [7,11,12]. Therefore, the bacterin and/or toxoid [22] containing B. bronchiseptica and toxigenic capsular serotype D P. multocida were developed, and these vaccines were prophylactic against experimental AR with B. bronchiseptica and toxigenic capsular serotype D P. multocida [1,14,24,36]. Although there is little information about toxigenic capsular serotype A P. multocida associated with AR [4,10,13,33], it has been suggested that the immunological quality of DNT of both serotype A and D P. multocida origin is identical by in vitro experiments [5,16], the toxin gene of serotypes A and D being identical [17].…”

mentioning

confidence: 99%

“…DNT of B. bronchiseptica and DNT of P. multocida have been recognized as a development of AR [7,11,12], and DNT of serotype A and D P. multocida origin were identical in an in vitro experiment [15,16]. Various vaccines containing bacterin and/or toxoid of B. bronchiseptica and DNT-producing serotype D P. multocida are being used worldwide, but these vaccines have been evaluated against experimental AR with B. bronchiseptica and DNT-producing serotype D P. multocida [1,14,24,36]. In the present study, piglets were challenged with B. bronchiseptica and DNT-producing serotype A P. multocida, and consequently all of non-vaccinated group showed severe or moderate nasal turbinate atrophy at slaughter, which has been described in reports on inoculation with B. bronchiseptica and toxigenic P. multocida serotype D [23,[27][28][29]32], but none of the pigs showed signs of AR.…”

mentioning

confidence: 99%

Effect of Bordetella bronchiseptica and Serotype D Pasteurella multocida Bacterin-Toxoid on the Occurrence of Atrophic Rhinitis after Experimental Infection with B. bronchiseptica and Toxigenic Type A P. multocida.

Sakano

Okada²,

Taneda³

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. In efficacy tests, 7 primary specific-pathogen-free piglets vaccinated with the Bordetella bronchiseptica and type D Pasteurella multocida bacterin-toxoid were challenged with B. bronchiseptica and type A P. multocida. Severe or moderate nasal turbinate atrophy was produced in the non-vaccinated pigs, whereas, only one of the 4 pigs in the vaccinated group had slight turbinate atrophy. Other immune sera against crude toxin of P. multocida type A or D were cross neutralized. bronchiseptica was done in a 0.5 ml suspension instilled into each nostril on the post-vaccination week (PVW) 4, and P. multocida was done in the same manner as with B. bronchiseptica starting from PVW 5 for four consecutive days. The pigs were observed daily for clinical signs of AR. Nasal swab samples were taken from all pigs immediately before vaccination and at regular time intervals after vaccination. For the recovery of B. bronchiseptica, samples were plated on MacConkey agar (Eiken Chemical Co., Ltd., Tokyo) containing 25 µg/ml of furazolidone, 0.5 µg/ml gentamicin, 4 µg/ml of fradiomycin and 2 µg/ml of clindamycin, and were incubated for 3 days at 37°C. The samples were also cultured for P. multocida using DS agar (Difco Lab.) containing 0.1 µg/ml gentamicin and 30 µg/ml vancomycin for 18 hr at 37°C. Suspected colonies of B. bronchiseptica or P. multocida were identified using conventional biochemical tests [6,21]. Blood samples were collected from pigs at the same times as the nasal swab samples were taken. ELISA tests for the detection of antibodies against pure DNT of B. bronchiseptica [8,9] and pure DNT of P. multocida serotype D [18] were performed. Each pure DNT antigen showed a single band of nearly 160 kd or 140 kd by sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis. The pigs were sacrificed using an intravenous injection of thiopental sodium (Tanabe Pharmaceutical Co., Osaka) on PVW 7. The snouts were cut transversely between the first molar and the canine tooth, and the turbinate atrophy was recorded as: = normal, + = slight, ++ = moderate and +++ = severe according to the criteria described by Maeda et al. [15].The relationship between clinical atrophic rhinitis (AR) of swine and infections with Bordetella bronchiseptica and/ or toxigenic capsular serotype D Pasteurella multocida has been reported by many investigators [2, 3, 23, 27-30, 32, 35]. In addition, it has been reported that capsular serotype A P. multocida is isolated predominantly from pneumonic lung in swine [26,37]. Previous experiments have shown that the dermonecrotic toxin (DNT) of B. bronchiseptica and P. multocida are virulence factors producing AR [7,11,12]. Therefore, the bacterin and/or toxoid [22] containing B. bronchiseptica and toxigenic capsular serotype D P. multocida were developed, and these vaccines were prophylactic against experimental AR with B. bronchiseptica and toxigenic capsular serotype D P. multocida [1,14,24,36]. Although there is little information about toxigenic capsular serotype A P. multocida associated wi...

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“…Nimierous experiments have directly supported the claim that lesions of PAR are signific2intly correlated with weight gain and/or feed efficiency during the growing and finishing periods (9,10,11,12,38,170,174). Other experiments have arrived at equivocal results, with negative correlations between PAR and ADG being significant tn only sections of some studies (14,120,130).…”

Section: Introductionmentioning

confidence: 80%

“…where Ngn pop = number needed to sample given a specific population size, Njuf pop = the number needed to sample given a population of infinite number and N = the number in the finite population This ELISA detects presence of the dermonecrotic toxin produced specifically by toxigenic P. multocida (58,170), solving the problem of serologic cross reactions with nontoxigenic strains and related bacteria.…”

Section: Sample Sizementioning

confidence: 99%

Transmission and immune response studies of toxigenic Pasteurella multocida

Sundberg

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Introduction Materials and Methods Results Culture and toxigenicity Restriction endonuclease analysis and , phenotypic typing Discussion 136 Bibliography VI. GENERAL CONCLUSION APPENDIX 146 ix ACKNOWLEDGEMENTS I have had the good fortune to have been associated with some people that I am proud to call my mentors and friends. Dr. Earl Bamawell, at the University of Nebraska-Lincoln, gave me the opportunity to observe how a university professor teaches, inside and outside of the classroom and laboratory. Dr. Eldon Uhlenhopp gave his time smd energy to help me leam how to give my practitioner experiences the thought and order needed to teach and do research. Dr. Lawrence E)vans kept me interested tn academia during practice and gave me the chance I needed to further my education and take advantage of new opportunities. Dr. George Beran guided me through my university experience, helping me focus on applied epidemiology. My father, Donald Sundberg, a true educator, has always taspired me as an example of wisdom and honesty. The experiences he gave me on the farm gave me the confidence to pursue my interests. My mother. Faith Bergqulst Sundberg, gave me a solid foundation for personal, professional, and spiritual growth and taught me the value of patience and perseverance. My bride, friend, and partner. Deb, has given me more than I could ever return. Without her support and love my life would be incomplete and my veterinary and graduate education would not have been possible. She has sacrificed her own professional career to give me the chance to advance mine. Her wonderful gifts of humor, sensitivity, insight and X intuition are unmatched. She has my heart and my respect and the respect of sill her professional and personal acquaintances. She gave us our daughters, Kristin, Kari, Lora, eind Molly. Their energy and enthusiasm eire our greatest pleasures. Each of them has such wonderful potential that is limited only by their imagination. They keep me going. They sire the true meaning of carpe diem. L GENERAL INTRODUCTION Dissertation Organization This dissertation consists of a literature review, three papers, a general summary, and an appendix. Paper three is to be submitted to the American Society for Microbiology publication. Journal of Clinical Microbiology, and is written in the appropriate format. Papers one sind two are to be submitted to the American Society for Microbiology publication. Infection and Immunity, and cire written in the corresponding format. The appendix contains data from two of the individual projects. Tables 1 through 18 from paper 1 (Chapter III) and Tables 19 through 31 from paper 2 (Chapter IV). beta-methyl butyrate as an adjunct to vaccination was investigated. The local antibody response to respiratory infection and its usefulness as an indication of pathogen transmission among pigs was studied. Finadly, restriction endonuclease digestion was used to confirm organism identification in transmission studies among cohort pigs.

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Evaluation of an atrophic rhinitis vaccine under controlled conditions

Cited by 4 publications

References 0 publications

Atrophic rhinitis vaccine composition triggers different serological profiles that do not correlate with protection

Atrophic rhinitis vaccine composition triggers different serological profiles that do not correlate with protection

Effect of Bordetella bronchiseptica and Serotype D Pasteurella multocida Bacterin-Toxoid on the Occurrence of Atrophic Rhinitis after Experimental Infection with B. bronchiseptica and Toxigenic Type A P. multocida.

Transmission and immune response studies of toxigenic Pasteurella multocida

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