Transmission and immune response studies of toxigenic Pasteurella multocida

Abstract

Introduction Materials and Methods Results Culture and toxigenicity Restriction endonuclease analysis and , phenotypic typing Discussion 136 Bibliography VI. GENERAL CONCLUSION APPENDIX 146 ix ACKNOWLEDGEMENTS I have had the good fortune to have been associated with some people that I am proud to call my mentors and friends. Dr. Earl Bamawell, at the University of Nebraska-Lincoln, gave me the opportunity to observe how a university professor teaches, inside and outside of the classroom and laboratory. Dr. Eldon Uhlenhopp gave his time smd energy to help me leam how to give my practitioner experiences the thought and order needed to teach and do research. Dr. Lawrence E)vans kept me interested tn academia during practice and gave me the chance I needed to further my education and take advantage of new opportunities. Dr. George Beran guided me through my university experience, helping me focus on applied epidemiology. My father, Donald Sundberg, a true educator, has always taspired me as an example of wisdom and honesty. The experiences he gave me on the farm gave me the confidence to pursue my interests. My mother. Faith Bergqulst Sundberg, gave me a solid foundation for personal, professional, and spiritual growth and taught me the value of patience and perseverance. My bride, friend, and partner. Deb, has given me more than I could ever return. Without her support and love my life would be incomplete and my veterinary and graduate education would not have been possible. She has sacrificed her own professional career to give me the chance to advance mine. Her wonderful gifts of humor, sensitivity, insight and X intuition are unmatched. She has my heart and my respect and the respect of sill her professional and personal acquaintances. She gave us our daughters, Kristin, Kari, Lora, eind Molly. Their energy and enthusiasm eire our greatest pleasures. Each of them has such wonderful potential that is limited only by their imagination. They keep me going. They sire the true meaning of carpe diem. L GENERAL INTRODUCTION Dissertation Organization This dissertation consists of a literature review, three papers, a general summary, and an appendix. Paper three is to be submitted to the American Society for Microbiology publication. Journal of Clinical Microbiology, and is written in the appropriate format. Papers one sind two are to be submitted to the American Society for Microbiology publication. Infection and Immunity, and cire written in the corresponding format. The appendix contains data from two of the individual projects. Tables 1 through 18 from paper 1 (Chapter III) and Tables 19 through 31 from paper 2 (Chapter IV). beta-methyl butyrate as an adjunct to vaccination was investigated. The local antibody response to respiratory infection and its usefulness as an indication of pathogen transmission among pigs was studied. Finadly, restriction endonuclease digestion was used to confirm organism identification in transmission studies among cohort pigs.