Evaluation of Acute Toxicity Study and Diuretic Activity of Ural Syrup

Abstract

There are considerable amount of scientific evidences available for diuretic activity of individual ingredients of a polyherbal formulation Ural Syrup however no evidence has been found which proves overall safety and efficacy of the formulation. The present study was undertaken to investigate safety as well as Diuretic activity of this polyherbal formulation-Ural Syrup. Acute toxicity study was carried out as per OECD Guideline 420. The Ural syrup (1.8mL/kg and 3.6mL/kg) was tested for its diuretic activity on rat model. Hydrochlorothiazide (HCTZ) (10mg/kg) was used as positive control in study. The diuretic effect of the Ural syrup was evaluated by measuring urine volume, pH and excretion of sodium and potassium content. In result, significant increase in urine volume was observed at both dosage levels of Ural syrup in comparison to normal control group. There was no significant change observed in pH of urine. The excretion of Sodium was also found significantly increased at both dosage levels but more in TEDx2 group with respect to TED group. Potassium excretion was found increased only at TEDx2 group as compared to normal control group. It can be concluded that Ural syrup produced considerable diuretic effect which appeared to be comparable to that produced by the reference diuretic HCTZ. There was no lethality and toxic reaction found among the tested animals. The present study provides scientific support that Ural syrup can be employed as a safe and effective diuretic drug.

Evaluation of Physico Chemical Properties and Diuretic Activity of Biotite Calx (Abhraka Bhasma) Processed With Tribulus Terrestris L.: An Experimental Study

“…Urine output was observed, collected and recorded hourly. At the end of 2 nd , 4 th , 6 th and 8 th hours urine was collected and measured and the pH of each group of animals urine were determined 20,21 .Then animals were taken out of the cages and the total volume of urine excreted by each group was noted 22,23 .…”