Objective: To evaluate the Acute toxicity study and Anti-asthmatic activity of Zeal Herbal Granules. Materials & Methods: In the present study, acute toxicity study was carried out as per OECD guideline 423. Anti-asthmatic activity of Zeal Herbal Granules was investigated against compound 48/80-induced mast cell degranulation. Percentage mast cell degranulation was calculated at different concentration level i.e.1, 10, 100 and 1000μg/mL. Results: Zeal Herbal Granules showed significant protection of rat mesenteric mast cells from disruption caused by compound 48/80. Significant dose dependent effect was observed in percentage mast cell degranulation at different dose level of Zeal Herbal Granules in comparison to negative control. 26.83% mast cell degranulation was observed at 100μg/mL dose level of Zeal Herbal Granules. Conclusion: The present study revealed that Zeal Herbal Granules has significant anti-asthmatic activity against compound 48/80-induced mast cell degranulation comparable to that produced by Ketotifen fumarate. There was no lethal and toxic reactions found among the tested animals. Zeal Herbal Granules can be a safe and effective drug for patient with asthmatic complaints.

India has long tradition of using Ayurvedic medicines as therapeutic agent. In comparison to rapid growth of herbal market, export rate of traditional Indian medicine is quite insignificant. It might be due to lack of proper quality control measures of raw materials and formulation. So, it is essential to ensure quality, safety and efficacy of raw materials and formulation. Rasayana Churna is a classical Ayurvedic formulation, which comprises dried powders of three well known rejuvenating drugs viz. dried stem of Guduchi (Tinospora cordifolia Miers.), dried fruit of Gokshur (Tribulus terrestris Linn.) and dried pericarp of Amalaki (Emblica officinalis Gaertn.) in equal proportion. It is used in Ayurveda as Rasayana to enhance general body resistance, promote longevity, as anti-stress and adaptogen. Therapeutic activity of Rasayana Churna may be attributed to cordifolioside A, diosgenin and gallic acid. Cordifolioside A is an active constituent of Guduchi (Tinospora cordifolia Miers.). Diosgenin is steroidal saponin present in Gokshur (Tribulus terrestris Linn.) and gallic acid is a phenolic compound present in Amalaki (Emblica officinalis Gaertn.). Several research work have reported the quantitative estimation of active constituent in individual drug but no established method was found for quantitative analysis of Rasayana Churna. This is the first attempt ever regarding quantitative estimation of Rasayana Churna by RP-HPLC. Various validated methods were used for estimation of cordifolioside A, diosgenin and gallic acid. These RP-HPLC methods can be used for routine quality control of raw materials and Rasayana Churna.

Nociceptive pain arises from the stimulation of specific pain receptors. These receptors respond to heat, cold, vibration, stretch and chemical stimuli released from damaged cells. A use of non-steroidal anti-inflammatory drugs (NSAIDs) is routine in the management of pain. Although effective at reducing pain and inflammation, long term use of NSAIDs is associated with known side effects. Herbal and Ayurvedic products are widely perceived as safe due to their natural origin and long historical clinical use. Hence in the present study, an attempt was made to evaluate acute toxicity and analgesic activity of an Ayurvedic proprietary formulation "Plugit Capsule" on experimental animals. Acute toxicity study was carried out as per OECD guideline 423. Analgesic activity was evaluated by hot plate and tail flick method. Selected animals were divided in to four groups in both experimental models. Test drug was evaluated for two different dose levels and administered orally at 260 mg/kg (TED-I) and 520 mg/kg (TED-II) in Group 2 and 3 respectively. Diclofenac sodium was used as reference standard. The animals did not manifest any signs of toxicity and mortality at both dose level 2000 mg/kg and 5000 mg/kg. Plugit Capsule at TED-II showed more significant analgesic effect in both hot plate and tail flick nociceptive tests with respect to TED-I. On the basis of study data it can be concluded that Plugit Capsule produces central analgesic effect without any side effect hence can be employed as a safe and effective alternative to NSAIDs for the treatment of painful conditions.

There are considerable amount of scientific evidences available for diuretic activity of individual ingredients of a polyherbal formulation Ural Syrup however no evidence has been found which proves overall safety and efficacy of the formulation. The present study was undertaken to investigate safety as well as Diuretic activity of this polyherbal formulation-Ural Syrup. Acute toxicity study was carried out as per OECD Guideline 420. The Ural syrup (1.8mL/kg and 3.6mL/kg) was tested for its diuretic activity on rat model. Hydrochlorothiazide (HCTZ) (10mg/kg) was used as positive control in study. The diuretic effect of the Ural syrup was evaluated by measuring urine volume, pH and excretion of sodium and potassium content. In result, significant increase in urine volume was observed at both dosage levels of Ural syrup in comparison to normal control group. There was no significant change observed in pH of urine. The excretion of Sodium was also found significantly increased at both dosage levels but more in TEDx2 group with respect to TED group. Potassium excretion was found increased only at TEDx2 group as compared to normal control group. It can be concluded that Ural syrup produced considerable diuretic effect which appeared to be comparable to that produced by the reference diuretic HCTZ. There was no lethality and toxic reaction found among the tested animals. The present study provides scientific support that Ural syrup can be employed as a safe and effective diuretic drug.

This study aimed to investigate the potenital effect of an Ayurvedic herbo-mineral formulation in a rat model of testosterone propionate-induced benign prostatic hyperplasia (BPH). Methods: Male Wistar rats weighing 180-250 g were used. The selected animals were divided into six groups where each group consisted of six animals. Experimentally BPH was developed by subcutaneous administration of testosterone propionate (3 mg/kg) dissolved in corn oil for 28 days. The tested herbo-mineral formulation was administered orally for 28 consecutive days at 90 mg/kg, once or twice daily in different groups. On 29 th day, Blood samples were collected to evaluate different parameters like blood urea nitrogen (BUN), serum creatinine, serum total protein, serum total cholesterol, serum albumin, serum globulin and serum A/G ratio. Prostate glands were dissected out for different prostatic parameters and histopathological examination. Results: The herbo-mineral formulation showed significant effect on urine output and different prostatic parameters. Significant changes in urine volume, prostate weight, prostate weight index, prostate length and prostate width were observed. In addition, positive effects on different biochemical parameters like BUN, serum creatinine, serum total protein, etc were recorded. The used formulation, at both dose levels, also showed significant reversal in histology of prostate glands when compared with disease control group. On the other hand, no side effects were observed according to the administered herbo-mineral formulation. Conclusion: The findings of this study concludes that Herbo-mineral formulation has promising effect on testosterone propionate-induced BPH in male wistar rats.

Background Standardization of polyherbal medicine though being the need of the hour is a toilsome task. Among the various methods employed for quality control and standardization of polyherbal medicine, phytochemical profiling is of utmost importance as it signifies the quality as well as efficacy of the medicine. The work was aimed to develop and validate a simple, quick, and accurate RP-HPLC technique for simultaneous assessment of monoammonium glycyrrhizinate and sennoside-B in a polyherbal laxative tablet. The phytomarkers were effectively quantified by RP-HPLC system on C18 analytical column using gradient mobile phase consisting of phosphate buffer to acetonitrile with the detector wavelength set at 254 nm. This developed method was validated by determination of parameters like accuracy, linearity, precision, limit of detection, and quantification as well as robustness according to ICH guidelines. Results Calibration curve of both phytomarkers showed excellent linear correlation coefficients. LOD and LOQ were also calculated by equation. Precision studies were carried out using intra-day and inter-day intervals and RSD values were found to be less than 2.00%. The method was found to be accurate, which was evident from 98.96 to 101.39% and 99.17 to 100.67% recovery of monoammonium glycyrrhizinate and sennoside-B, respectively, when the formulation was spiked with the respective phytomarkers. Conclusion The validated method can be employed as standardization tool for herbal formulations with accuracy and precision. The developed method will assist in maintaining the good quality and batch to batch uniformity of polyherbal formulations containing Yashtimadhu, Swarnpatri, and Aragvadha as active ingredients.

The stress is generally considered as the functional adaptation of the organism in order to cope up with changing and challenging environment. Exposure to stressful conditions is among the most common human experience. It has been reported that exposure to physical and mental stress situations can stimulate numerous pathways, leading to increased production of oxygen free radicals. In such situation, the antioxidant defense systems of body may become insufficient to scavenge all free radicals. In the present study, an attempt was made to evaluate acute toxicity of a herbo-mineral Ayurvedic formulation, Ranger capsule along with its in-vitro anti-oxidant property and in-vivo anti-stress activity. Acute toxicity study was carried out on wistar rats as per OECD Guidelines 423. For anti-stress activity forced swimming endurance test was carried out on wistar rats. During this study, Difference in rectal temperature and swimming time was noted. Blood samples were collected for estimation of serum cortisol and serum glucose level. The reduction potential of Ranger Capsule was determined by the invitro ferric reducing anti-oxidant power (FRAP) assay at various concentration. Study data revealed that Ranger Capsule didn't produced any toxic effect even up to the dose level of 5000 mg/kg hence was proven as safe. The pre-treatment of Ranger Capsule prevented the fall of rectal temperature in highly significant manner and increased swimming time in comparison to stress control group. Ranger Capsule treated animals showed significant restriction in elevation of serum cortisol and serum glucose level as compared to stress control group. From the available results, it can be concluded that pre-treatment of Ranger Capsule is safe, capable to increase tolerance against non-specific stress in experimental animals and having significant anti-oxidant activity.

Large number of people around the world is suffering from hair loss (alopecia) or hair thinning. Though hair loss is not a life threatening health issue it can make life distressful and can thereby affect physical and mental health of human being. To find solution of such problems, an attempt has been made to evaluate the hair growth promotion activity of a herbo-mineral Ayurvedic formulation, Trichup Capsule. Safety of drug was also evaluated by conducting acute toxicity study as per OECD guideline. For evaluation of hair growth promoting activity, selected animals were divided in to three groups where each group was consisting of six animals. TED and TED × 2 group was treated with Trichup Capsule at 171 mg/kg/day and 342 mg/kg/day, p.o., for 30 days respectively. Visual parameters, hair length, hair diameter and serum total protein were determined. At the end of the study, animals did not manifest any signs of toxicity or mortality. Treatment of Trichup Capsule showed significant effect on all visual parameters. Length and diameter of hairs were significantly increased in TED and TED × 2 groups. Serum total protein was also observed to be elevated in TED and TED × 2 groups in comparison to normal control. Present study data revealed that Trichup Capsule is safe and having significant hair growth promoting activity.