Evaluation of a novel microplate colorimetric hybridization genotyping assay for human papillomavirus

Barcellos, Regina Bones; Almeida, Sabrina Esteves de Matos; Sperhacke, Rosa Dea; Verza, Mirela; Rosso, Franciele; Medeiros, Rúbia Marília de; Perizzolo, Paulo Fernado; Cortez-Herrera, Elizabeth; Rossetti, Maria Lúcia Rosa

doi:10.1016/j.jviromet.2011.06.010

Cited by 15 publications

(19 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There is a paucity of data on these genotypes in HIV positive women with abnormal cytology, notwithstanding their potential enhanced role in cervical dysplasia development in HIV positive patients [15, 16]. …”

Section: Introductionmentioning

confidence: 99%

Multiple HPV infections in female sex workers in Western Kenya: implications for prophylactic vaccines within this sub population

Menon

Broeck

Rossi³

et al. 2017

Infect Agents Cancer

View full text Add to dashboard Cite

Background Whilst the imputed role of High Risk (HR) HPV infection in the development of cervical lesions and cancer has been established, the high number of HPV genotypes that Female Sex workers (FSW) harbour warrants that the synergistic effects of potential HR (pHR) and HR HPV genotypes be elucidated to assess the potential impact of prophylactic vaccines. This population in Kenya also harbours a number of other vaginal infections and STIs, including bacterial vaginosis (BV), trichomonas vaginalis (TV) and candida spp.The aims of this cross-sectional analysis in Kenya are to explore the epidemiology of abnormal cytology and the pairing of pHR/HPV genotypes in HIV-negative and HIV-infected FSW.MethodsA cross-sectional study design of 616 FSW from Western Kenya aged between 18 and 61 years during 2009–2015 using a peer recruitment sampling strategy.ResultsOf the 599 FSW who underwent cytological examination, 87 had abnormal cytology (14.5%; 95% CI: 12.0–17.6%). A combined prevalence of HPV16 and 18 (29.6%; 95% CI: 22.2–37.8%) was observed in abnormal cytology. HPV 53 and 51 were the most observed pairing in FSW with abnormal cytology. Significant adjusted associations were found between abnormal cytology and TV (aOR: 30; 95% CI: 14.1–62.9), multiple HR HPV (aOR: 3.7; 95% CI: 1.9–7.3), HPV 51 (aOR 3.7; 95% CI 1.6–8.6) and HPV 52 (aOR 6.1; 95% CI: 2.8–13.3).ConclusionHPV 51 and 52 were independently associated with abnormal cervical cytology in both HIV negative/positive FSW. The strong association between TV and cervical dysplasia and the high percentage of FSW harbouring more than one STI underscore the need for enhanced STI management within the framework of cervical cancer prevention.

show abstract

Section: Introductionmentioning

confidence: 99%

Multiple HPV infections in female sex workers in Western Kenya: implications for prophylactic vaccines within this sub population

Menon

Broeck

Rossi³

et al. 2017

Infect Agents Cancer

View full text Add to dashboard Cite

show abstract

“…HPV DNA genotyping of high-risk types 16, 18, 31, 33, 39, 45 and 59 was carried out using a microplate colorimetric hybridization assay (MCHA) developed in our laboratory (Barcellos et al , 2011). Denatured PCR amplicons were reverse hybridized to the probes fixed on a microplate.…”

Section: Methodsmentioning

confidence: 99%

Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology

et al. 2014

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7%) were positive for HPV-DNA and MN, respectively. Evidence for MN was found in 11 out of 20 (55%) HPV-DNA positive samples and in 36 out of 138 (26.1%) HPV-DNA negative ones. MN presence was significantly higher in HPV-DNA positive samples (p = 0.016). On the other hand, the absence of MN observed in a considerable number of HPV-DNA negative samples (102) may be of great value in predicting the absence of HPV. The mean age of HPV-DNA positive women (34.2 ± 12.6) was significantly lower than the mean age of HPV-DNA negative women (43.9 ± 13.7) (p = 0.003). Infection by one or multiple HPV types was found in 11 out of 20 (55.0%) and 9 out of 20 (45.0%) samples, respectively. The evaluation of MN using cervical smears collected for cytology tests could, thus, be used as additional information to monitor a population’s exposure to HPV.

show abstract

“…However, this assay does not amplify HPV -35 and -53, the cost is still relatively high, and it requires specific apparatus [33-35]. …”

Section: Introductionmentioning

confidence: 99%

“…The MCHA (Boehringer Mannheim, Germany) is a method for identifying six HR-HPV (-16, -18, -31, -33, -39 and -45) and is based on the amplification by PCR of the 150 bp fragment within the L1 region by consensus primers GP5+/6+, followed by colorimetric hybridization to six type-specific probes on microwell plates (Immobilizer™ Amino Surface, Nunc, Roskilde, Denmark) [35]. …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A review of methods for detect human Papillomavirusinfection

Abreu

Souza

Gimenes

et al. 2012

Virol J

216

181

View full text Add to dashboard Cite

Human Papillomavirus (HPV) is the most common sexually transmitted virus. Worldwide, the most common high-risk (HR)-HPV are -16/18, and approximately 70% of cervical cancers (CC) are due to infection by these genotypes. Persistent infection by HR-HPV is a necessary but not sufficient cause of this cancer, which develops over a long period through precursor lesions, which can be detected by cytological screening. Although this screening has decreased the incidence of CC, HPV-related cervical disease, including premalignant and malignant lesions, continues to be a major burden on health-care systems. Although not completely elucidated, the HPV-driven molecular mechanisms underlying the development of cervical lesions have provided a number of potential biomarkers for both diagnostic and prognostic use in the clinical management of women with HPV-related cervical disease, and these biomarkers can also be used to increase the positive predictive value of current screening methods. In addition, they can provide insights into the biology of HPV-induced cancer and thus lead to the development of nonsurgical therapies. Considering the importance of detecting HPV and related biomarkers, a variety of methods are being developed for these purposes. This review summarizes current knowledge of detection methods for HPV, and related biomarkers that can be used to discriminate lesions with a high risk of progression to CC.

show abstract

Evaluation of a novel microplate colorimetric hybridization genotyping assay for human papillomavirus

Cited by 15 publications

References 44 publications

Multiple HPV infections in female sex workers in Western Kenya: implications for prophylactic vaccines within this sub population

Multiple HPV infections in female sex workers in Western Kenya: implications for prophylactic vaccines within this sub population

Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology

A review of methods for detect human Papillomavirusinfection

Contact Info

Product

Resources

About