Abstract:The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7%) were positive for HPV-DNA and MN, respectively. Evidence for MN was found in 11 out of 20 (55%) HP… Show more
“…Although the mutagenic potential of both BPV [27,28,74,139] and HPV [29,30,140] is known, the molecular mechanisms that induce clastogenesis remains unknown. Mutagenic studies have attributed the BPV clastogenic potential to combined action of viral oncoproteins (E5, E6 and E7), that induce cell hyperproliferation and replication fork collapse [26,27,74,141].…”
Section: Oncogenic Virus and Ros Generation: A Field To Explorementioning
confidence: 99%
“…For this reason, mutagen identification has been explored [13][14][15][16], since to avoid the long term exposure to these mutagens can be considered a protective method against cancer. Currently, it is known that several drugs [16][17][18], organic [19][20][21][22] and inorganic chemical compounds [23,24] and biological agents, including viruses [12,[25][26][27][28][29][30][31][32][33] are able to induce DNA damage.…”
“…Although the mutagenic potential of both BPV [27,28,74,139] and HPV [29,30,140] is known, the molecular mechanisms that induce clastogenesis remains unknown. Mutagenic studies have attributed the BPV clastogenic potential to combined action of viral oncoproteins (E5, E6 and E7), that induce cell hyperproliferation and replication fork collapse [26,27,74,141].…”
Section: Oncogenic Virus and Ros Generation: A Field To Explorementioning
confidence: 99%
“…For this reason, mutagen identification has been explored [13][14][15][16], since to avoid the long term exposure to these mutagens can be considered a protective method against cancer. Currently, it is known that several drugs [16][17][18], organic [19][20][21][22] and inorganic chemical compounds [23,24] and biological agents, including viruses [12,[25][26][27][28][29][30][31][32][33] are able to induce DNA damage.…”
“…Papillomaviruses (PVs) are a group of viruses with epithelium and mucous tropism [ 1 , 2 ]. PVs can infect all vertebrates, including rabbits [ 3 – 5 ], dogs [ 6 , 7 ], goats [ 8 ], humans [ 9 – 12 ], and bovines [ 1 , 13 – 15 ]. In the last decades, an increasing interest in studies involving these viruses has been observed [ 16 , 17 ].…”
Bovine papillomavirus (BPV) is considered a useful model to study HPV oncogenic process. BPV interacts with the host chromatin, resulting in DNA damage, which is attributed to E5, E6, and E7 viral oncoproteins activity. However, the oncogenic mechanisms of BPV E6 oncoprotein per se remain unknown. This study aimed to evaluate the mutagenic potential of Bos taurus papillomavirus type 1 (BPV-1) E6 recombinant oncoprotein by the cytokinesis-block micronucleus assay (CBMNA) and comet assay (CA). Peripheral blood samples of five calves were collected. Samples were subjected to molecular diagnosis, which did not reveal presence of BPV sequences. Samples were treated with 1 μg/mL of BPV-1 E6 oncoprotein and 50 μg/mL of cyclophosphamide (positive control). Negative controls were not submitted to any treatment. The samples were submitted to the CBMNA and CA. The results showed that BPV E6 oncoprotein induces clastogenesis per se, which is indicative of genomic instability. These results allowed better understanding the mechanism of cancer promotion associated with the BPV E6 oncoprotein and revealed that this oncoprotein can induce carcinogenesis per se. E6 recombinant oncoprotein has been suggested as a possible vaccine candidate. Results pointed out that BPV E6 recombinant oncoprotein modifications are required to use it as vaccine.
“…Exfoliated epithelial cells have been used in cytology to detect abnormal cells and to evaluate the diameters of nuclei and cytoplasm for cytomorphometric analysis and MN presence [8,9,10]. Samanta et al [11], Rosin and Anwar [8], Cortés-Gutiérrez et al [12] and Cassel et al [13] reported that infectious agents and cancer affect MN frequency in human cells. MN frequency was increased on cervicovaginal smears in human papillomavirus and Schistosoma haematobium infections, cervical intraepithelial lesions and carcinoma [8,11,12,13].…”
Section: Introductionmentioning
confidence: 99%
“…Samanta et al [11], Rosin and Anwar [8], Cortés-Gutiérrez et al [12] and Cassel et al [13] reported that infectious agents and cancer affect MN frequency in human cells. MN frequency was increased on cervicovaginal smears in human papillomavirus and Schistosoma haematobium infections, cervical intraepithelial lesions and carcinoma [8,11,12,13]. Although candidiasis is the most common fungal disease in the world, little information is known about the structural profile of epithelial cells in genital candidiasis.…”
Objective:Candida is the most common cause of fungal infections. The aim of this study was to fill the gaps in the current knowledge on the frequencies of micronuclei and nuclear anomalies, and the nucleus/cytoplasmic ratio in genital candidiasis. Study Design: A total of 88 Papanicolaou- stained cervical smears, which comprised Candida spp. (n = 44) and control cases with no infectious agent (n = 44), were studied. In each smear, cells with micronuclei and nuclear anomalies were counted in 1,000 epithelial cells and also nuclear and cellular areas were evaluated using image analysis software at a magnification of ×400. Results: The frequencies of micronucleated and binucleated cells and cells with perinuclear halos, and the nucleus/cytoplasmic ratio of epithelial cells were higher in the Candida-infected group compared with the control group (p < 0.05). Conclusions: Genital candidiasis is able to induce changes in the size and shape of epithelial cells. The nuclear/cytoplasmic ratio and the frequency of micronuclei may reflect the DNA damage in the cervical epithelium. Micronucleus scoring could be used to screen the genomic damage profile of epithelial cells in candidiasis.
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