Evaluation of a monoclonal antibody-based enzyme-linked immunosorbent assay for detection of Campylobacter fetus in bovine preputial washing and vaginal mucus samples

Brooks, Brian W.; Devenish, John; Lutze-Wallace, Cyril; Milnes, D.; Robertson, Ruth; Berlie-Surujballi, Gloria

doi:10.1016/j.vetmic.2004.07.008

Cited by 90 publications

(46 citation statements)

References 21 publications

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“…Indeed, surface-exposed immunodominant molecules, such as flagellar proteins (Fernando 2007) and the O-antigen, undergo genetic drift to avoid immunological detection. An alternative target molecule is the core oligosaccharide of the LOS, which has been successfully targeted in EIA assays to detect C. fetus (Brooks 2002(Brooks , 2004Devenish 2005). High rates of continuous expression of the antigen is another important consideration, especially in Campylobacter cells that undergo stress during food production and processing.…”

Section: Wwwintechopencommentioning

confidence: 99%

Immunological Methods for the Detection of Campylobacter spp. - Current Applications and Potential Use in Biosensors

Oyarzábal¹,

Battie²

2012

Trends in Immunolabelled and Related Techniques

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Section: Wwwintechopencommentioning

confidence: 99%

Immunological Methods for the Detection of Campylobacter spp. - Current Applications and Potential Use in Biosensors

Oyarzábal¹,

Battie²

2012

Trends in Immunolabelled and Related Techniques

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“…Esses anticorpos foram escolhidos porque persistem por mais tempo e sua concentração permanece constante no trato genital por muitos meses (Hewson et al 1985, Hum et al 1994. Brooks et al (2004) utilizaram anticorpos monoclonais contra LPS de C. fetus, em ELISA direto, mas não obtiveram bom limite de detecção (10 4 a 10 6 UFC/mL), tornando inviável a utilização da técnica com estes anticorpos monoclonais. Também foram desenvolvidos e são utilizados ensaios de PCR multiplex para diferenciar o C. fetus subsp.…”

Section: Diagnóstico Clínico E Laboratorialunclassified

Campilobacteriose genital bovina e tricomonose genital bovina: epidemiologia, diagnóstico e controle

et al. 2011

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Pesq. Vet. Bras. 31(4): 336-344, abril 2011 RESUMO.-A presente atualização trata de duas das mais importantes doenças sexualmente transmitidas de bovinos, a campilobacteriose genital bovina e a tricomonose genital bovina. São abordados aspectos relacionados à epidemiologia destas doenças, principalmente em relação a sua distribuição no Brasil. Também são revisados aspectos importantes de diagnóstico, incluindo as técnicas e interpretação dos resultados, além de medidas de controle para ambas as doenças. INTRODUÇÃOA campilobacteriose genital bovina (CGB) e a tricomonose genital bovina (TGB) são doenças infecto-contagiosas de transmissão sexual, que acometem bovinos em idade reprodutiva e causam grandes perdas econômicas em decorrência dos problemas reprodutivos desencadeados (Dekeyser 1984, Goodger & Skirrow 1986 The present update deals with two of the most important sexually transmitted diseases of cattle: bovine genital campylobacteriosis and bovine genital trichomonosis. Epidemiological aspects, mainly their distribution in Brazil, alongside with their diagnosis in cattle are presented and commented. The main points in their diagnoses, including the description of the techniques and the interpretation of the results are also reviewed. Finally the control and prevention of both diseases are discussed.

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“…The isolation and identification of the organism by culture is accepted as "gold standard" method to confirm the diagnosis of C. fetus infection; however, the reduced viability of the agent in clinical samples could be affecting the obtained results (Brooks et al 2004). Other methods such as direct fluorescent antibody test, cervical mucus agglutination, and enzyme-linked immunosorbent assay (ELISA) are also used for diagnosis; however, they present limitations in sensitivity and specificity (Hum et al 1994, Brooks et al 2004, McFadden et al 2005.…”

Section: Introductionmentioning

confidence: 99%

“…Other methods such as direct fluorescent antibody test, cervical mucus agglutination, and enzyme-linked immunosorbent assay (ELISA) are also used for diagnosis; however, they present limitations in sensitivity and specificity (Hum et al 1994, Brooks et al 2004, McFadden et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Polymerase chain reaction for the diagnosis of bovine genital campylobacteriosis

et al. 2010

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RESUMO.-[Reação em cadeia da polimerase para o diagnóstico de campilobacteriose genital bovina.] Campilobacteriose genital bovina é uma doença venérea comum em bovinos. A prevalência desta doença pode ser subestimada na maioria das vezes pela natureza microaeróbica do agente etiológico, Campylobacter fetus subspecies venerealis. O propósito do presente estudo foi avaliar a utilização da reação de polimerase em cadeia (PCR) no diagnóstico de campilobacteriose genital em amostras obtidas de aspirado prepucial de touros, muco cervical de vacas e conteú-do abomasal de fetos abortados, coletados em meio enriquecido. Cinco protocolos diferentes de extração de DNA foram testados: termo extração, lise com proteinase K, lise com guanidine isothiocyanate, lise com DNAzol e lise com hexadeciltrimetilamônio brometo (CTAB). A especificidade, sensibilidade e a aplicação da técnica da PCR foram também avaliadas com amostras clínicas e comparadas com bactérias isoladas por cultura padrão. DNA extraído pelo protocolo de CTAB demonstrou os melhores resultados na PCR, e foi capaz de detectar 63 unidades formadoras de colônias de C. fetus por ml de meio. Bovine genital campylobacteriosis is a common venereal disease of cattle; the prevalence of this disease can be underestimated mostly because of the nature of the etiological agent, the microaerobic Campylobacter fetus subspecies venerealis. The purpose of the current study was to evaluate the utilization of polymerase chain reaction (PCR) in the diagnosis of genital campylobacteriosis in samples obtained from bull prepuce aspirate, cow cervical mucus, and abomasum contents of aborted fetuses, collected into enrichment medium. Five different DNA extraction protocols were tested: thermal extraction, lysis with proteinase K, lysis with guanidine isothiocyanate, lysis with DNAzol, and lysis with hexadecyltrimethylammonium bromide (CTAB). The specificity, sensitivity, and technical application of the PCR assay were also evaluated with clinical samples and compared to bacterial isolation by standard culture. DNA extraction by the CTAB protocol provided better results in PCR, and it was able to detect 63 colony-forming units per ml of C. fetus.Out of 277 clinical samples tested, 68 (24%) were positive for Campylobacter fetus using PCR, while only 8 (2.8%) of the samples were positive by bacterial isolation in solid medium, proving the superiority of the PCR technique when compared to the standard isolation method, and providing evidence for its usefulness as a better screening test in cattle for the diagnosis of bovine genital campylobacteriosis. amostras foram positivas por isolamento bacteriológico, provando a superioridade da técnica de PCR quando comparada com métodos padrão de isolamento, e fornecendo evidências de sua utilização como um teste de melhor projeção para diagnóstico em campilobacteriose genital bovina.

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Evaluation of a monoclonal antibody-based enzyme-linked immunosorbent assay for detection of Campylobacter fetus in bovine preputial washing and vaginal mucus samples

Cited by 90 publications

References 21 publications

Immunological Methods for the Detection of Campylobacter spp. - Current Applications and Potential Use in Biosensors

Immunological Methods for the Detection of Campylobacter spp. - Current Applications and Potential Use in Biosensors

Campilobacteriose genital bovina e tricomonose genital bovina: epidemiologia, diagnóstico e controle

Polymerase chain reaction for the diagnosis of bovine genital campylobacteriosis

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