Evaluation of a dot ELISA kit for measuring immunoglobulin M antibodies to canine parvovirus and distemper virus

Abstract

A dot ELISA for the detection of immunoglobulin M (IgM) antibodies to canine distemper virus (CDC) and canine parvovirus (CPV) was assessed. The titres of IgM antibodies to CDV and CPV in 100 dogs were measured by the Immunocomb ELISA kit and compared with the results derived from the immunofluorescence assay (IFA). There was a strong correlation between the results of the dot ELISA technique and the IFA (P < 0.001). The dot ELISA kit was also used to assess the changes in the levels of immunoglobulin G (IgG) and IgM antibodies to CPV and CDV in 10 puppies vaccinated with a polyvalent vaccine. High levels of IgM antibodies to CPV were first detected seven days after they were vaccinated, and after nine days all the pups had high titres of IgG antibodies to CPV. High levels of IgM antibodies to CDV were detected after nine days and the highest average titres were recorded after 12 days. IgG antibodies to CDV were present from nine days after vaccination.

Isolation and Typing of Canine Parvovirus in CRFK Cell Line in Puducherry, South India

“…The presence of the virus in cell culture fluids at third passage levels was also detected by dot-ELISA [13] using CPV hyper immune serum (1:100) raised in rabbits. Anti rabbit IgG HRPO conjugates were used in 1:100 dilutions.…”

“…Development of brown color spots after 5-10 min of substrate and chromogen administration indicated positive reactions. CPV vaccine (Megavac-P) and cell cultured fluids from uninfected controls were used as positive and negative controls, respectively [13]. Indirect fluorescent antibody technique for the detection of CPV don in the Leighton tubes containing monolayer of CRFK cell lines infected with CPV samples at third passage level were subjected to IFAT 4 days post-inoculation to detect the presence of virus infection.…”

Canine Parvovirus: Current Perspective

“…The double sandwich ELISA is a rapid, simple, sensitive and suitable test over ELISA for routine diagnostic use for detection of CPV antigen in canine faeces. The ELISA test has become the most common test for parvovirus in puppies [97].…”

Antibody study in canine distemper virus nucleocapsid protein gene-immunized mice

“…Reaction conditions were determined based on previous studies (Waner et al, 2003). The OD 490 value was measured after the reaction.…”