Antibody study in canine distemper virus nucleocapsid protein gene-immunized mice

Yuan, Bo; Li, X Y; Zhu, Tingxian; Lin, Yuan; Hu, Jun; Chen, J; Gao, Wa; Ren, W Z

doi:10.4238/2015.april.10.20

Cited by 5 publications

(4 citation statements)

References 11 publications

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“…The final nested PCR products were cloned into pMD-18 T vector according to reference [ 21 ], except the temperature of the target segment ligation to the pMD-18 T vector was changed to room temperature for 1 h. Then, commissioning Beijing Rui Bo Xing ke Biological Technology company to sequence. The sequencing results were blasted at NCBI ( https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome ), and HPV16 integration sites were determined in human chromosome for every integration specimen, severally.…”

Section: Methodsmentioning

confidence: 99%

Integration of human papillomavirus 16 in esophageal carcinoma samples

HaiE

et al. 2017

Infect Agents Cancer

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BackgroundEsophageal carcinoma (EC) is one of the major cancers in China. In 1982, Syrjanen first hypothesized the relationship between human papillomavirus (HPV) infection and the development of esophageal cancer. Since then, many reports in the field have supported this viewpoint. This study investigated the etiological relationship between HPV infection and the occurrence of esophageal carcinoma at Tangshan City of the Hebei province in China.Methods189 samples of esophageal carcinoma patients were collected. DNA and RNA were isolated from samples, HPV DNA was detected by polymerase chain reaction (PCR) using My09/11 for HPV L1, and HPV16 was determined using type-specific primer sets for HPV16 E6. The HPV16 integration site was verified by amplification of papillomavirus oncogene transcripts, and HPV16 oncogene transcript products were ligated to the pMD-18 T vector and sequenced to confirm the physical location of HPV16 integration.Results168 HPV-positive samples were detected in 189 samples, and among them 76 specimens were HPV16 positive. Approximately 600 bp of the HPV16 oncogene transcript were detected in nine esophageal cancer samples. Sequence analysis revealed that HPV16 E7 integrated into human chromosome 2 in three samples, into human chromosome 5 in one sample, into human chromosome 6 in one sample, into human chromosome 8 in two samples, and into human chromosome 17 in two samples. The results verified that the integrated HPV16 E7 in five samples harbored one mutation of viral DNA compared with the HPV16 sequence provided in GenBank (K02718).ConclusionsThe high prevalence of HPV16 suggests that HPV16 may play an etiological role in the development of esophageal cancer. The integration of HPV16 into host cell chromosomes suggests that persistent HPV infection is key for esophageal epithelial cell malignant transformation and carcinogenesis.

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Section: Methodsmentioning

confidence: 99%

Integration of human papillomavirus 16 in esophageal carcinoma samples

HaiE

et al. 2017

Infect Agents Cancer

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“…To further confirm specific HPV52 oncogene transcription in all of the samples, the final nested PCR products were cloned into a pMD-18T vector according to a previous study ( 31 ), except the temperature of the target segment ligation to the pMD-18T vector was changed to room temperature for 1 h. Then Beijing Rui Bo Xing ke Biological Technology (Beijing, China) was commisioned to sequence the products. The results were analyzed using the National Center for Biotechnology Information BLAST ( ), and HPV52 integration sites in human chromosomes were determined for every integration specimen.…”

Section: Methodsmentioning

confidence: 99%

Analysis of human papilloma virus type 52 integration status in exfoliated cervical cells

Zhang

Liu

et al. 2017

Exp Ther Med

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To explore the significance of human papilloma virus type 52 (HPV52) infection and its integration in cells within cervical lesions, the expression levels of HPV52 were detected using polymerase chain reaction (PCR). The copy numbers of HPV52 E2, HPV52 E6 and the reference gene β-actin were determined by quantitative PCR to analyze the association between HPV52 integration and cervical lesions. HPV52 integration was analyzed by the amplification of papillomavirus oncogene transcripts. A total of 13 samples from 468 cases were positive for HPV52. Among the samples, 1 case with an E2/E6 ratio >1 was purely episomal, 3 cases with an E2/E6 ratio of 0 were purely integrated, and 9 cases with an E2/E6 ratio of between 0 and 1 were a mixture of integrated and episomal. With the progression of cervical disease, the prevalence of the episomal type decreased gradually, and the prevalence of the integrated (episomal and integrated) forms increased. The pure integration of HPV52 occurred in chromosomes 2, 5 and 8. These results indicate that HPV52 integration into the host genome may be a key factor in cervical lesions. Thus, patients at high risk for cervical lesions may potentially be identified by screening for HPV52 infection and integration.

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“…Based on Bayesian model algorithm, the haplotypes with high confidence were deduced by PHASE, and the number of iterations is ≥ 100 (Stephens et al, 2001). All the inferred haplotypes were verified by clonal sequencing, and the process was described previously (Yuan et al, 2015). The effect of amino acid substitutions on the protein function was evaluated via program PANTHER (http://www.pantherdb.org/; Mi et al, 2017).…”

Section: Pcr and Sequencingmentioning

confidence: 99%

Polymorphisms of Beta Casein (Csn2) CDS and Inference of Its Variants in River and Swamp Water Buffalo (Bubalus Bubalis)

2021

THE JAPS

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β-casein (β-CN) has an important effect on surface properties of casein micelles and milk-clotting properties. However, current understanding of buffalo CSN2 gene polymorphisms is not sufficient. In this study, the polymorphisms in the complete coding sequence (CDS) of buffalo CSN2 were detected using PCR product direct sequencing. The CDS lengths of CSN2 gene in river and swamp buffalo were the same, which was 675 nucleotides and encoded a peptide with 224 amino acid residues. A total of 15 single nucleotide polymorphisms (SNPs) were identified in two types of buffalo. Among them, c.72C>T, c.161A>G, c.167C>T, c.168A>G, c.236G>C, c.249C>G, c.350T>C and c.391G>T were nonsynonymous, which leads to the changes of p.Gly10Ser, p.Gln39Arg, p.Thr41Met, p.Gly64Ala, p.Asn68Lys, p.Met102Thr and p.Val116Phe in the mature peptide of buffalo β-CN, respectively. However, it was predicted that these substitutions had no effect on the function of buffalo β-CN. Fourteen haplotypes were defined based on the SNPs found in buffalo, and accordingly, 4 protein variants and 6 synonymous variants were added in the genetic variants of buffalo β-CN, named variant B 2 , B 3 , B 4 , C, C 1 , C 2 , C 3 , D, E and F, respectively. The main variant in river buffalo was variant B, whereas in swamp buffalo was variant C. All the variants determined in buffalo did not exist in the animals of Bos genus. In addition, there were 11 amino acid differential sites of β-CN between buffalo and Bos genus, of which Thr at residue 41 was located at the phosphorylation site. Furthermore, the results revealed that both types of buffalo β-CN were "A 2like", which indicates that the β-CN in buffalo milk is beneficial to human health.

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Antibody study in canine distemper virus nucleocapsid protein gene-immunized mice

Cited by 5 publications

References 11 publications

Integration of human papillomavirus 16 in esophageal carcinoma samples

Integration of human papillomavirus 16 in esophageal carcinoma samples

Analysis of human papilloma virus type 52 integration status in exfoliated cervical cells

Polymorphisms of Beta Casein (Csn2) CDS and Inference of Its Variants in River and Swamp Water Buffalo (Bubalus Bubalis)

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