Evaluation of 2,4-D and Dicamba genotoxicity in bean seedlings using comet and RAPD assays

Cenkci, Süleyman; Yıldız, Mustafa; Çiğerci, İbrahim Hakkı; Bozdağ, Ahmet; Terzi, Hakan; Terzi, Evrim Suna Arikan

doi:10.1016/j.ecoenv.2010.07.033

Cited by 56 publications

(41 citation statements)

References 28 publications

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“…The damaging activity of dicamba in different mammalian cell targets was also previously observed by the same research group (Gonzàlez et al 2006(Gonzàlez et al , 2007(Gonzàlez et al , 2009). The results of these studies and some others (Amer and Aly 2001;Cenkci et al 2010) are in agreement with the results of our study, although different cell types and/or test system were used.…”

Section: Discussionsupporting

confidence: 82%

“…Unfortunately, to our best knowledge, there is no study available on the genotoxic and/ or cytotoxic effects of NAAm on humans in the literature. However, some synthetic auxins, such as 2,4-dichlorophenoxyacetic acid (2,4-D) (Holland et al 2002;Cenkci et al 2010), dicamba (Gonzàlez et al 2007(Gonzàlez et al , 2009Gonzàlez et al 2011), and synthetic IAA (Salopek-Sondi et al 2010) have been found genotoxic, mutagenic and cytotoxic in various test systems. Thus, taking into account these facts, it has become important to evaluate the possible genotoxic potential of NAAm on humans.…”

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confidence: 99%

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In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

Kocaman

Güven

2015

Cytotechnology

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1-Naphthaleneacetamide (NAAm) is a synthetic plant growth regulator in the auxin family that is widely used in agriculture to promote the growth of numerous fruits, for root cuttings and as a fruit thinning agent. The potential genotoxic effects of NAAm were investigated in vitro by the chromosome aberrations (CAs), and cytokinesis-block micronucleus assays in human peripheral blood lymphocytes (PBLs) for the first time. The human PBLs were treated with 20, 40, 80, and 160 lg/mL of NAAm for 24 and 48 h. The results of this study showed that NAAm significantly induced the formation of structural CA and MN for all concentrations (20, 40, 80 and 160 lg/mL) and treatment periods (24 and 48 h) when compared with the negative and the solvent control. In addition, the higher concentrations of NAAm (80 and 160 lg/mL) caused a statistically significant increase in nuclear bud (NBUD) formation for both 24 and 48 h treatment times. With regard to the cell cycle kinetics, at all the tested concentrations, NAAm caused a statistically significant reduction in the mitotic index (MI) only for 48 h treatment period and also in the nuclear division index (NDI) for both 24 and 48 h treatment periods as compared to the control groups. The reductions in the MI and NDI occured in a concentration-dependent manner for both treatment times. In conclusion, the present results indicate that in the tested experimental conditions, NAAm was genotoxic and cytotoxic on human PBLs in vitro.

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Section: Discussionsupporting

confidence: 82%

mentioning

confidence: 99%

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

Kocaman

Güven

2015

Cytotechnology

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“…A number of researchers have shown the genotoxic potential of 2,4-D that were investigated by using different test systems including chromosome aberration assay, micronucleus and comet assay technique that monitor genotoxic effects on plants (25)(26)(27)(28). The advantage of measuring the direct effects of genotoxins on DNA mainly depends on its sensitive and non-time consuming properties (25).…”

Section: Resultsmentioning

confidence: 99%

Environmental risk assessment under the pollutants exposure with using four lichen species and molecular assay in cement plant, Aşkale-Erzurum (Turkey)

Hamutoğlu¹,

Aslan²,

Aras³

et al. 2016

Turk Hij Den Biyol Derg

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ÖZET Amaç:Aşkale Following the exposure to the pollutants of 1, 2 and 3 district situated at a distance of 50, 100, 200m to the cement factory, P. furfuracea with a control bands were observed respectively. Moreover 31, 13 and 15 bands from the control species disappeared in sites 1, 2 and 3 in P. furfuracea samples. Furthermore, the highest polymorphism value was obtained (P% = 86,6%) in U. longissima and L. pulmonaria by the OPC04 primer, and the lowest polymorphism was

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“…Some studies have used the comet, micronucleus, or chromosome aberration assays to measure the effects of heavy metals and other genotoxic agents on plants (Cenkci et al, 2010). Advantages of measuring effects of genotoxic chemicals directly on DNA are mainly related to the sensitivity and short response time.…”

Section: Discussionmentioning

confidence: 99%

“…Genotoxicity as a result of metal toxicity is also described to play a major role in DNA damage induction (Halliwell, 1990). Toxic chemicals induce several cellular stress responses and damage different cellular components such as membranes, proteins, and DNA (Patra & Panda, 1998;Waisberg et al, 2003;Cenkci et al, 2010;Altınözlü et al, 2012;Tran & Popova, 2013).…”

Section: Introductionmentioning

confidence: 99%

Heavy metal accumulation and genotoxicity indicator capacity of the lichen species Ramalina pollinaria collected from around an iron steel factory in Karabük, Turkey

Cansaran-Duman¹,

Altunkaynak²,

Aras³

2014

Turk J Bot

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With fast economic development and industrialization, a vast range of genotoxic chemicals are produced and distributed into the environment. These chemicals adversely affect living organisms and often lead to serious diseases in human beings. Due to the highly conserved structure of the genetic material, it is possible to use a broad variety of species, including bacteria, yeasts, animals, and plants, in genotoxicity tests (Poli et al., 1999). In recent years, lichens have been used as good bioindicators of the genetic toxicity of environmental pollutants (Aras et al., 2010;Cansaran-Duman et al., 2011). Genotoxicity as a result of metal toxicity is also described to play a major role in DNA damage induction (Halliwell, 1990). Toxic chemicals induce several cellular stress responses and damage different cellular components such as membranes, proteins, and DNA (

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Evaluation of 2,4-D and Dicamba genotoxicity in bean seedlings using comet and RAPD assays

Cited by 56 publications

References 28 publications

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

Environmental risk assessment under the pollutants exposure with using four lichen species and molecular assay in cement plant, Aşkale-Erzurum (Turkey)

Heavy metal accumulation and genotoxicity indicator capacity of the lichen species Ramalina pollinaria collected from around an iron steel factory in Karabük, Turkey

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