Environmental risk assessment under the pollutants exposure with using four lichen species and molecular assay in cement plant, Aşkale-Erzurum (Turkey)

Abstract

ÖZET Amaç:Aşkale Following the exposure to the pollutants of 1, 2 and 3 district situated at a distance of 50, 100, 200m to the cement factory, P. furfuracea with a control bands were observed respectively. Moreover 31, 13 and 15 bands from the control species disappeared in sites 1, 2 and 3 in P. furfuracea samples. Furthermore, the highest polymorphism value was obtained (P% = 86,6%) in U. longissima and L. pulmonaria by the OPC04 primer, and the lowest polymorphism was

Accumulation of Cr6+, Pb2+ and Cd2+ and Ultraviolet Radiation Alter Methylation and Genomic Dna Status in Ramalina Farinaceae

“…Environmental stressors such as heavy metal pollution can induce mutations and toxic effects, leading to the disruption of DNA integrity [40]. Therefore, the determination of DNA damage in terrestrial organisms living in polluted areas is a considerable exponent in ecotoxicology studies [6,41]. As a result of the comparing of band differences of RAPD and Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) analysis, it was determined that the rate of change in the methylation model of the samples exposed to heavy metal stress varies more than the samples exposed to UV radiation.…”

“…Several studies have been published to determine the genotoxicity with DNA molecular markers of heavy metals exposure in lichens. Hamutoğlu et al [6] observed that there were 19, 45 and 51 bands in P. furfuracea (control band number 83), respectively, after the 1st, 2nd and 3rd regions, located 50, 100 and 200m away from the cement factory that was exposed to contaminants. Sorrentino et al [42] investigated with ISSR molecular markers in moss Sphagnum palustre for showing both Cd and Pb salts a genotoxic effect in a dose-dependent manner.…”

“…Thanks to these properties, lichens have the ability to absorb elements such as heavy metals emitted from pollutant sources [3]. In recent studies, lichen species have been identified as suitable organisms that can be used to reflect atmospheric rates by measuring pollutants such as metals, elements and radionuclides accumulated in their thalli [4,5,6]. Particularly, epiphytic lichens are considered to be one of the important bioindicators of air quality because they easily obtain water and essential nutrients from the air [7][8][9][10].…”

“…The contamination of soil with heavy metals has a genotoxic effect on plants that have to grow in these areas, causing changes in the DNA profile, such as mutation [27]. Recently, RAPD and Amplified Fragment Length Polymorphism (AFLP) techniques have been successfully utilized to identify temporary DNA changes caused by heavy metal stress [6,16,[28][29][30][31][32]. Current molecular genetics and genomic studies have provided insight into the importance of cytosine methylation, which plays a significant role in gene regulation [33].…”

Lobaria pulmonaria (L.) Hoffm.: The Multifaceted Suitability of the Lung Lichen to Monitor Forest Ecosystems

“…Lobaria pulmonaria has been employed as a monitoring tool (as a biomonitor and/or as a bioindicator) in studies carried out in forest habitats around specific pollution sources [50][51][52][53][54]117,118]; in remote forest sites [55][56][57]; and in sites concerned by the long-range transportation of pollutants, such as radionuclides [77][78][79][80][81][82], acid rain depositions [119,120], or under controlled conditions in relation to specific pollutants [58][59][60][61][62][63].…”