Évaluation de l’activité antifongique de Mitracarpus scaber, une rubiaceae codifiée MISCA sur Candida glabrata

Kporou, Elisée Kouassi; Kra, Mathieu Koffi Adou; Ouattara, Sié; Guédé-Guina, F.

doi:10.2515/therapie/2010013

Cited by 9 publications

(15 citation statements)

References 8 publications

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“…The ethanolic extract of Terminalia glaucescens was effective on twelve (12) isolates including E. coli, E. aerogenes, K. pneumoniae and P. mirabilis from urine and blood samples, with zone of inhibition ranging from 9.5 -20 mm. The ethanolic extract of Mangifera indica showed potency on fifteen (15) isolates including E. coli, E. aerogenes, K. pneumoniae and P. mirabilis from urine and blood samples, with zone of inhibition ranging from 9.5 -18.5 mm.…”

Section: Antibacterial Screening Of Plant Materialsmentioning

confidence: 99%

“…In various parts of tropical Africa, it is traditionally used for treatment of sore throat, ringworm and eczema, fresh cuts, wounds and ulcer (9,10,11). Previous studies also revealed that the plant contains biologically active substances such as fatty acids, flavonoids and other phenolic compounds with potential antifungal, antimicrobial and anti-inflammatory activities (12,13,14,15,16,17).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial activity of Terminalia glaucescens, Mangifera indica and Mitracarpus villosus on carbapenem-resistant enterobacteriaceae

Gbala¹,

Anibijuwon²

2018

Af J Clin Exp Micro

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The root of Terminalia glaucescens, stem-bark of Mangifera indica and leaves of Mitracarpus villosus were screened for antibacterial activities against 23 carbapenem-resistant Enterobacteriaceae (CRE) isolates. The phyto-constituents of the plants were extracted by cold maceration. Disc-diffusion and broth microdilution methods were used to determine the antibacterial activity and the minimum inhibitory concentration, respectively. The sensitivity of the isolates to the methanol extracts of the plant parts was between four to eight isolates (10 -26.5 mm) including Enterobacter aerogenes, Proteus mirabilis and Escherichia coli, with the highest activity shown by Mitracarpus villosus. An overall higher activity was however observed with the ethanol extracts of the plant parts with potency on twelve to fifteen isolates (9 -18.5 mm) including Enterobacter aerogenes, Proteus mirabilis, Escherichia coli and Klebsiella pneumoniae. Generally for all methanol extracts, a constant MIC value 100 mg/ml was observed for the susceptible isolates except two Enterobacter aerogenes isolates with MIC of 1 mg/ml while the MIC value of the ethanol extracts ranged from ≤ 0.1 -100 mg/ml.

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Section: Antibacterial Screening Of Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of Terminalia glaucescens, Mangifera indica and Mitracarpus villosus on carbapenem-resistant enterobacteriaceae

Gbala¹,

Anibijuwon²

2018

Af J Clin Exp Micro

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“…Tous ces problèmes liés aux antifongiques usuels ont amené de nombreuses équipes scientifiques à rechercher de nouveaux antifongiques. C'est aussi pour répondre à cette importante préoc-cupation que nous avons initié des investigations sur le pouvoir anticandidosique de Mitracarpus scaber [5,12,14]. C'est une plante de la pharmacopée ivoirienne largement utilisée en médecine traditionnelle dans le traitement de nombreuses affections (buccodentaires, hépatites, affections cutanées et affections digestives) [12,15,17,19,21].…”

Section: Introductionunclassified

Amélioration par fractionnement chromatographique de l’activité anticandidosique d’un extrait hexanique de Mitracarpus scaber Zucc. sur la croissance in vitro de Candida albicans et Candida tropicalis

Kporou¹,

Kra²,

Ouattara³

et al. 2010

Phytothérapie

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Dans le but d'apporter notre contribution à la lutte contre les candidoses opportunistes, nous avons testé dix-sept fractions chromatographiques d'un extrait hexanique de Mitracarpus scaber codifié Misca sur la croissance in vitro de Candida albicans et Candida glabrata. Les tests antifongiques ont été réalisés sur milieu Sabouraud auquel l'extrait végétal a été incorporé selon la méthode de la double dilution en tubes penchés. Les concentrations varient de 3 125 à 12 µg/ml. Les résultats ont montré que dans cet intervalle de valeurs, seules les fractions F 6 , F 7 , F 8 ont permis une nette inhibition de la croissance des deux souches de Candida à 781 µg/ml. Parmi elles, la fraction F 8 possède la meilleure activité anticandidosique avec une CI 50 = 16 µg/ml sur Candida albicans contre CI 50 = 45 µg/ml sur Candida tropicalis qui est donc la souche la moins sensible. La fraction F 8 de l'extrait hexanique est donc celle qui concentre le mieux les principes actifs anticandidosiques de Misca. Mots clés : Misca -Activité anticandidosique -Fractionnement chromatographique Improvement by chromatographic dividing of anticandidosic activity of an hexanic extract Mitracarpus scaber on the in vitro growth of Candida albicans and Candida tropicalisAbstract: In the goal to bring our contribution to the struggle against opportunistic candidosis, we tested seventeen chromatographic fraction gotten from an hexanic extract of a Mitracarpus scaber Misca codified on the in vitro growth of Candida albicans and Candida tropicalis. Antifungal tests have been made on Sabouraud medium culture. Extract has been inserted to this medium according to the Agar slant method. The range of concentrations has been fixed between 3.125 µg/ml and 12 µg/ml. Results showed that in this interval of values, alone fractions F 6 , F 7 , F 8 permitted a clean inhibition to 3 125 µg/ml of the growth of the 2 strains of Candida. Among them, the fraction F 8 has possessed a better anticandidosic activity with IC 50 = 16 µg/ml on Candida albicans against IC 50 = 45 µg/mls on Candida tropicalis which is the least sensible. So, the chromatographic fraction F 8 obtained from a hexanic extract is the one that permits a better concentration of active principles of Misca.

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“…6,[13][14][15] Antifungal activity has been evaluated on C. albicans, C. glabrata and C. tropicalis in 2009 and 2010. [16][17][18][19] This study completes data on anticandidosic spectrum of organic extracts of this plant in comparison to ketoconazole, a standard antifungal. To achieve this general objective, the following specific objectives are defined: to prepare vegetable extracts and to assess antifungal activity of these extracts.…”

Section: Introductionmentioning

confidence: 70%

“…After incubation time, colonies were directly counted with a counter colonies per extract type and the growth in 9 experimental tubes was evaluated as a percentage of survival, calculated on 100% survival in the growth control tube (1). 19 The processing of these data allowed to determine the values of MIC (minimal inhibitory concentration), MFC (minimal fungicidal concentration) and IC 50 (concentration for 50% inhibition).…”

Section: Antifungal Activity Assaymentioning

confidence: 99%

Antifungal potential of improved crude extracts of Mitracarpus scaber (Zucc) against Candida guilliermondii and Candida parapsilosis

Kporou¹,

Coulibaly

Ackah³

et al. 2017

PBE

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Objective: The present study was undertaken to evaluate antifungal potential of improved crude extracts of Mitracarpus scaber Zucc., an ivorian traditional medicinal plant against two species of clinical yeast isolates: Candida parapsilosis and Candida guilliermondii. Methods:For evaluation of antifungal activity, double dilution method in slope tubes was used. Crude extracts of M. scaber were prepared by extracting with a blender in aqueous and hydroalcohol. In first; Aqueous (X Aq ) and Hydroalcoholic (X 0 ) extracts were evaluated, and secondary the crude extract X 0 was improved by partition in mixture of aqueous and hexanic solvent 50:50 v/v. Each dried phase was also examined. A phytochemical screening has been performed on most active extracts. Results:In vitro antifungal activity showed that crude extract X 0 was the most active with MIC = 6.25 mg/ml. Among the extracts obtained by partition from extract X 0 , extract X 11 (Hexanic phase) was the better with lowest antifungal parameters MIC and MFC range between 1.562 mg/ml and 0.781 mg/ml and IC 50 range between 0.73 mg/ml to 0.598 mg/ml. In addition, the most active extract contained only terpens and steroids.Conclusions: Antifungal activity of M. scaber was improved by partition of an hydroalcoholic extract (X 0 ) in mixture hexane-water 50:50 v/v. Hexanic phase (X 11 ) was more active against C. parapsilosis and C. guilliermondii.

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Évaluation de l’activité antifongique de Mitracarpus scaber, une rubiaceae codifiée MISCA sur Candida glabrata

Cited by 9 publications

References 8 publications

Antibacterial activity of <i>Terminalia glaucescens</i>, <i>Mangifera indica</i> and <i>Mitracarpus villosus</i> on carbapenem-resistant enterobacteriaceae

Antibacterial activity of <i>Terminalia glaucescens</i>, <i>Mangifera indica</i> and <i>Mitracarpus villosus</i> on carbapenem-resistant enterobacteriaceae

Amélioration par fractionnement chromatographique de l’activité anticandidosique d’un extrait hexanique de Mitracarpus scaber Zucc. sur la croissance in vitro de Candida albicans et Candida tropicalis

Antifungal potential of improved crude extracts of Mitracarpus scaber (Zucc) against Candida guilliermondii and Candida parapsilosis

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