Dans le but d'apporter notre contribution à la lutte contre les candidoses opportunistes, nous avons testé dix-sept fractions chromatographiques d'un extrait hexanique de Mitracarpus scaber codifié Misca sur la croissance in vitro de Candida albicans et Candida glabrata. Les tests antifongiques ont été réalisés sur milieu Sabouraud auquel l'extrait végétal a été incorporé selon la méthode de la double dilution en tubes penchés. Les concentrations varient de 3 125 à 12 µg/ml. Les résultats ont montré que dans cet intervalle de valeurs, seules les fractions F 6 , F 7 , F 8 ont permis une nette inhibition de la croissance des deux souches de Candida à 781 µg/ml. Parmi elles, la fraction F 8 possède la meilleure activité anticandidosique avec une CI 50 = 16 µg/ml sur Candida albicans contre CI 50 = 45 µg/ml sur Candida tropicalis qui est donc la souche la moins sensible. La fraction F 8 de l'extrait hexanique est donc celle qui concentre le mieux les principes actifs anticandidosiques de Misca.
Mots clés : Misca -Activité anticandidosique -Fractionnement chromatographique
Improvement by chromatographic dividing of anticandidosic activity of an hexanic extract Mitracarpus scaber on the in vitro growth of Candida albicans and Candida tropicalisAbstract: In the goal to bring our contribution to the struggle against opportunistic candidosis, we tested seventeen chromatographic fraction gotten from an hexanic extract of a Mitracarpus scaber Misca codified on the in vitro growth of Candida albicans and Candida tropicalis. Antifungal tests have been made on Sabouraud medium culture. Extract has been inserted to this medium according to the Agar slant method. The range of concentrations has been fixed between 3.125 µg/ml and 12 µg/ml. Results showed that in this interval of values, alone fractions F 6 , F 7 , F 8 permitted a clean inhibition to 3 125 µg/ml of the growth of the 2 strains of Candida. Among them, the fraction F 8 has possessed a better anticandidosic activity with IC 50 = 16 µg/ml on Candida albicans against IC 50 = 45 µg/mls on Candida tropicalis which is the least sensible. So, the chromatographic fraction F 8 obtained from a hexanic extract is the one that permits a better concentration of active principles of Misca.
KEYWORDS
Terminalia superb
Anticandidosic Ellagic acid derivative
ABSTRACTThe aim of the present study was to optimize the anticandidosic activities of Terminalia superba (TEKAM4) and the identification of major compounds present in the most active chromatographic fraction. The hydroethanolic extract TEKAM4-X0 was prepared by homogenization employing a blender. Two derivatives extracts of TEKAM4-X0 (X1-1 and X1-2) were obtained by a liquid/liquid partition of TEKAM4-X0 in a mixture of hexane and water (v/v). Three chromatographic fractions (F1, F2 and F3) from X1-2 were separated by means of Sephadex-LH20 gel filtration chromatography. All the extracts were incorporated to Sabouraud according to the agar slanted double dilution method. Ketoconazole was used as standards for antifungal assay. The entire fractions were tested on the previously prepared medium culture containing 1000 cells of C. albicans. Antifungal activity was determined by evaluating antifungal parameters values (MFC and IC50). Lastly, the structures of 2 isolated compounds were elucidated by combination of Flash chromatography and spectroscopic methods, including MS, and multiple stage RMN experiments.
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