Evaluation and quantitative analysis of different growth periods of herb–arbor intercropping systems using HPLC and UV–vis methods coupled with chemometrics

Abstract

As a result of the pressure from population explosion, agricultural land resources require further protecting and rationally utilizing. Intercropping technique has been widely applied for agricultural production to save cultivated area, improve crop quality, and promote agriculture economy. In this study, we employed high-performance liquid chromatography (HPLC) and ultraviolet-visible spectroscopy (UV-vis) combined with chemometrics for determination and qualitative evaluation of several kinds of intercropping system with Gentiana rigescens Franch. ex Hemsl. (GR), which is used as an hepatic protector in local communities in China. Results revealed that GR in a Camellia sinensis intercropping system contained most gentiopicroside, sweroside, and total active constituents (six chemical indicators), whose content reached 91.09 ± 3.54, 1.03 ± 0.06, and 104.05 ± 6.48 mg g(-1), respectively. The two applied quantitative and qualitative methods reciprocally verified that GR with 2 years of growth period performed better in terms of quality than 1 year, collectively.

Comprehensive Quality Assessment Based Specific Chemical Profiles for Geographic and Tissue Variation in Gentiana rigescens Using HPLC and FTIR Method Combined with Principal Component Analysis

“…That samples were divided into three groups along the habitats was not found even overlapped completely, regardless of tissues. Therefore, the likelihood that associated plants influenced chemical constituents in GR was extremely low, which was adverse with the result of study by Chu et al ( 2016 ). We emphasize here that this result has been tentatively discovered through samples collected from natural setting.…”

“…Analyses were performed on an Agilent 1260 HPLC system (Agilent Technologies, Santa Clara, California, USA) composed of a G1315D diode-array detector (UV-vis, DAD, 190-400 nm), a G1311C VL quaternary gradient pumps equipped with a vacuum degasser, a G1316A thermostatted column compartment and a G1329B ALS auto sampler. The modified version of our published HPLC method (Chu et al, 2016 ) was used as separation conditions in this study. The separation was carried out on Agilent Intersil-C 18 column (150 × 4.6 mm, 5 μm).…”

Investigation of a Medical Plant for Hepatic Diseases with Secoiridoids Using HPLC and FT-IR Spectroscopy for a Case of Gentiana rigescens

“…Each of the 5 µL was injected into an Agilent 1260 HPLC system (Agilent Technologies, Santa Clara, California, USA) equipped with a G1315D diode-array detector. The separation HPLC method was altered based on a method outline in a published article [14]. Chromatographic separation was carried out using a Shimadzu Intersil-C 18 column (150 × 4.6 mm, 5 µm).…”

“…Various analytical methods including high performance thin-layer chromatography [13], high performance liquid chromatography (HPLC) [14], capillary electrophoresis [15], and liquid chromatography-mass spectrometry [16] have been used to determine the nature of secoridosides and its derivative. While some of these methods have complicated operations, their long analytical times remain useful and have been studied.…”