Evaluation and Optimization of Effective-dose of Alloxan for Inducing Type-2 Diabetes Mellitus in Long Evans Rat

Rahman, Shaikh Shahinur; Yasmin, Nilufar; Rahman, Abu Taher Mohammad Mizanur; Zaman, A.; Rahman, Mohammad Hafizur; Rouf, Shakh M. A.

doi:10.5530/ijper.51.4s.96

Cited by 9 publications

(7 citation statements)

References 14 publications

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“…It should be noted that according to the literary data glucose level in the blood of animals with alloxaninduced diabetic rat models depends on the individual dose and the method of administration of alloxan (intravenous, intraperitoneal, subcutaneously). According to different literary evidence intraperitoneal administration in the following doses: 100 mg/kg 24,25 , 150 mg/kg 26 , 160-170 mg/kg 20,27 200 mg/kg 28,29,30 of body weight in different solvents are preferred, which are the models to study a long-term toxic action of the drug. As a rule, purified water, 0.9% NaCl solution and acetate buffer (pH=4.5) are used as solvents to prepare alloxan for injections.…”

Section: Resultsmentioning

confidence: 99%

“…Factor analysis found disorders of inter-systemic relations of the adrenocortical and reninangiotensin systems in experimental diabetes which indicates disintegration of regulatory systems 27 . This disintegration can be a result of uncontrolled oxidative stress.…”

Section: Absolutementioning

confidence: 99%

“…Under conditions of alloxan-induced diabetic rat model against the ground of stable hypoinsulinemia in the dynamics of pathological process development, a vector direction of metabolic processes is found for the preservation of hyperglycemia. Hyperglycemia is directly associated with increased activity of SGOT in the liver tissue evidenced by a number of studies 27,59,70 . Increased synthesis of glucocorticoids activates glucose synthesis from carbohydrate-free components by means of induction of mRNA synthesis of key enzymes of gluconeogenesis and aminotransferase.…”

Section: Ratio Of Enzymesmentioning

confidence: 99%

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Untitled

2019

IJPSR

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Alloxan diabetes mellitus (DM) simulates acute metabolic stress in a different direction, whose starting point is primary lysis of β-cells in the pancreas. Disorders in the system of the major insulin-induced regulatory mechanism occur, initiating imbalance in the system of a living organism generally. Primary changes occur on the level of metabolic ways of the cells of the pancreas and liver: redistribution of mitochondrial and microsomal processes of oxidation with possible synthesis of new isoenzymatic forms of important metabolic enzymes, disturbance of synthesis of regulatory proteins and, as a result, degenerative changes in the cellular structure (lysis, necrosis and apoptosis). Despite the numerous and even contradictory points of view, the theory of cell lysis with the increase of transaminases activity is still predominant. Cytolytic syndrome leads to the destruction of liver cells, the concentration of serum GOT increases dramatically (probably due to the mitochondrial fraction). Under conditions of alloxan-induced diabetic rat model intensification of oxidative stress processes occur provoking changes, which are typical for chronic hepatitis. Alloxan diabetes serves as a classic example of free radical pathology. The next probable link involved in this pathogenesis will be nervous system and general cerebral dysfunction of the body. Numerous disputable data are the evidence of neuroendocrine regulation of the triple system where enzymatic activity or concentration of metabolic indices is the result of many cascade reactions or associated parallel conductors in the system of involved organs, first of all the pancreas and liver. Alloxan diabetes is an example of prior activation of the enzymatic systems. It serves to ensure metabolic changes under conditions of increased generation of hydroxyl radicals and disorders of transmembrane transport both between organelles inside the cells and in the intercellular space. INTRODUCTION: The diabetes mellitus still remains nowadays an urgent issue despite the high social-economic development, which does not prevent Diabetes Mellitus (DM) type 1 and 2.

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Section: Resultsmentioning

confidence: 99%

Section: Absolutementioning

confidence: 99%

Section: Ratio Of Enzymesmentioning

confidence: 99%

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2019

IJPSR

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“…The weight of the mice was between 20–24 g . Initially, alloxan tetrahydrate was injected into mice, resulting in diabetes 13 , 14 . Afterwards, ten mice were randomly divided into two groups, five as the control group and five as the treatment group.…”

Section: Methodsmentioning

confidence: 99%

Pulse Voltage Electrical Stimulation for Bacterial Inactivation and Wound Healing in Mice with Diabetes

Tirono¹,

Hananto²,

Abtokhi³

2022

AJMB

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Background: Treatment of wounds in diabetes often gets less than perfect healing. One of the reasons for the difficulty in treating wounds in diabetes is the growth of aerobic and anaerobic bacteria. This study aims to determine the pulse voltage and treatment time that can optimally inactivate bacteria, and their effect on wound healing in mice suffering from diabetes. Methods: The study used electrical stimulation with a direct voltage of 10 volts given a pulse voltage of 50-80 volts, a width of 50 µs, and the number of pulses of 65 per second. The research samples were Staphylococcus aureus (S. aureus) and Pseudo-monas aeruginosa (P. aeruginosa) bacteria that grew on beef and mice (Mus musculus) with diabetes. The treatment for S. aureus and P. aeruginosa bacteria was carried out using a pulse voltage of 50-80 volts for 5-15 min/day and repeated for 3 days. Meanwhile, treatment of mice wounds was carried out with a pulse voltage of 80 volts for 15 min/day and repeated for 7 days. Results: The results showed that treatment with a pulse voltage of 50-80 volts and a treatment time of 5-15 min significantly reduced the number of S. aureus and P. aeruginosa bacteria in beef (p£0.05). Treatment with a pulse voltage of 80 volts for 15 min made beef free from bacteria. Meanwhile, treatment with a pulse voltage of 80 volts for 15 min per day for seven days resulted in the wound state of three mice in the maturation phase and two mice in the proliferation phase on day 8 with an average wound area of 0.108 cm 2. Conclusion: The treatment with a pulse voltage of 80 volts for 15 min made the beef sterile, the mice wounds healed quickly, and the mice not stressed. The higher the blood glucose level, the slower the wound healing process.

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“…Hal ini terjadi karena perlakuan I, II, dan III diinduksi aloksan sebagai agen diabetogenik. Rahman et al (2017) menyatakan, induksi aloksan dosis 140 mg/kg BB dapat menyebabkan kondisi hiperglikemia pada tikus dengan kadar gula darah mencapai 539,63 mg/dL. Grafik perubahan kadar gula darah disajikan pada Gambar 1.…”

Section: Kadar Gula Darahunclassified

Kajian Penggunaan Beras Analog yang Disubstitusi Glukomanan Porang (Amorphophallus Oncophyllus) Sebagai Antidiabetes Mencit yang Diinduksi Aloksan

Safitri,

Subekti,

Hidayati

et al. 2023

JAH

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Beras analog yang disubtitusi glukomanan porang (Amorphophallus Oncophylus) mengandung serat yang tinggi dan memiliki indeks glikemk yang rendah (43) sehingga baik dikonsumsi oleh penderita diabetes. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian beras analog yang disubtitusi glukomanan porang terhadap kadar gula darah, berat badan, dan konsumsi ransum pada mencit yang diinduksi aloksan. Percobaan disusun dalam rancangan acak lengkap (RAL) terdiri dari 4 kali perlakuan dengan 6 ulangan. Data yang diperoleh diuji keseragamannya dengan menggunakan uji Bartlett dan kemenambahan data diuji dengan uji Tuckey. Selanjutnya data dianalisis dengan sidik ragam untuk mendapatkan penduga ragam galat dan mengetahui pengaruh perlakuan, kemudian data dianalisis dengan menggunakan uji BNT pada taraf 5%. Hasil penelitian menunjukan bahwa pemberian beras analog yang disubtitusi glukomanan porang pada mencit yang diinduksi aloksan memberikan kadar gula darah sebesar 93,83 mg/dL, menurunkan berat badan 3,53 g, konsumsi ransum 3,45 g/hari, dan efisiensi ransum -14,80%.

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Evaluation and Optimization of Effective-dose of Alloxan for Inducing Type-2 Diabetes Mellitus in Long Evans Rat

Cited by 9 publications

References 14 publications

Untitled

Untitled

Pulse Voltage Electrical Stimulation for Bacterial Inactivation and Wound Healing in Mice with Diabetes

Kajian Penggunaan Beras Analog yang Disubstitusi Glukomanan Porang (Amorphophallus Oncophyllus) Sebagai Antidiabetes Mencit yang Diinduksi Aloksan

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