Evaluating differentiation potential of the human menstrual blood-derived stem cells from infertile women into oocyte-like cells

Sheikholeslami, Azar; Kalhor, Neda; Sheykhhasan, Mohsen; Jannatifar, Rahil; Sahraei, Seyedeh Saeideh

doi:10.1016/j.repbio.2020.100477

Cited by 8 publications

(14 citation statements)

References 41 publications

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“…The results of our study demonstrated that MenSCs from both groups, healthy volunteers, as well as patients with unexplained infertility, could be characterized by the high expression of mesenchymal stem cell markers (CD9, CD44, CD73, CD90 and CD166). Our data also coincide with the work of Sheikholeslami et al (2021) who showed that MenSCs from healthy and infertile women (having endometriosis or polycystic ovary syndrome) do possess similar CD marker patterns [16]. However, our study revealed that MenSCs obtained from patients with unexplained infertility are different from MenSCs from healthy volunteers in more pronounced expression of mesenchymal cell surface markers CD56, SUSD2.…”

Section: Discussionsupporting

confidence: 91%

Menstrual Blood-Derived Endometrial Stem Cells’ Impact for the Treatment Perspective of Female Infertility

Skliutė

Baušytė

Borutinskaitė

et al. 2021

IJMS

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When looking for the causes and treatments of infertility, much attention is paid to one of the reproductive tissues—the endometrium. Therefore, endometrial stem cells are an attractive target for infertility studies in women of unexplained origin. Menstrual blood stem cells (MenSCs) are morphologically and functionally similar to cells derived directly from the endometrium; with dual expression of mesenchymal and embryonic cell markers, they proliferate and regenerate better than bone marrow mesenchymal stem cells. In addition, menstrual blood stem cells are extracted in a non-invasive and painless manner. In our study, we analyzed the characteristics and the potential for decidualization of menstrual blood stem cells isolated from healthy volunteers and women diagnosed with infertility. We demonstrated that MenSCs express CD44, CD166, CD16, CD15, BMSC, CD56, CD13 and HLA-ABC surface markers, have proliferative properties, and after induction of menstrual stem cell differentiation into epithelial direction, expression of genes related to decidualization (PRL, ESR, IGFBP and FOXO1) and angiogenesis (HIF1, VEGFR2 and VEGFR3) increased. Additionally, the p53, p21, H3K27me3 and HyperAcH4 proteins’ expression increased during MenSCs decidualization, they secrete proteins that are involved in the regulation of the actin cytoskeleton, estrogen and relaxin signaling pathways and the management of inflammatory processes. Our findings reveal the potential use of MenSCs for the treatment of reproductive disorders.

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Section: Discussionsupporting

confidence: 91%

Menstrual Blood-Derived Endometrial Stem Cells’ Impact for the Treatment Perspective of Female Infertility

Skliutė

Baušytė

Borutinskaitė

et al. 2021

IJMS

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“…The apoptosis rate in the endometrial cells of women with endometriosis is decreased, suggesting that the survival rate of cells that reach the peritoneal cavity is higher in patients with progressive endometriosis. Sahraei et al's results suggest that the Bax/Bcl-2 ratio is significantly lower in E-MenSC than in NE-MenSC, which has been shown to have an important association with apoptosis, thus confirming the decreased apoptosis seen in endometriosis stromal cells [7,8,21,22].…”

Section: Mensc From Patients With Endometriosis (E-mensc) Display Dif...mentioning

confidence: 89%

“…Nevertheless, it is worth noting Nikoo et al's findings of higher CD29 expression levels in E-MenSC, which suggest that these cells might support attachment to the peritoneum. On the other hand, E-MenSC have shown a higher migratory capacity, shown by higher mRNA expression levels of the MMP-2 and MMP-9 genes in these cells when compared to NE-MenSC [7,8,21,22].…”

Section: Mensc From Patients With Endometriosis (E-mensc) Display Dif...mentioning

confidence: 92%

The Emerging Role of Menstrual-Blood-Derived Stem Cells in Endometriosis

2022

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The human endometrium has a complex cellular composition that is capable of promoting cyclic regeneration, where endometrial stem cells play a critical role. Menstrual blood-derived stem cells (MenSC) were first discovered in 2007 and described as exhibiting mesenchymal stem cell properties, setting them in the spotlight for endometriosis research. The stem cell theory for endometriosis pathogenesis, supported by the consensual mechanism of retrograde menstruation, highlights the recognized importance that MenSC have gained by potentially being directly related to the genesis, development and maintenance of ectopic endometriotic lesions. Meanwhile, the differences observed between MenSC in patients with endometriosis and in healthy women underlines the applicability of these cells as a putative biomarker for the early diagnosis of endometriosis, as well as for the development of targeted therapies. It is expected that in the near future MenSC will have the potential to change the way we manage this complex disease, once their long-term safety and effectiveness are assessed.

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“…Study revealed that MenSCs could differentiate into endometrial cells in vitro via appropriate medium contained with 17β-estradiol valerate, transforming growth factor-β (TGF-β)-1, epidermal growth factor (EGF), platelet-derived growth factor (PDGF)-BB ( 23 ). Some researchers found that there were some morphological changes in managed MenSCs, and they expressed oocyte-related genes (LHR, FSHR, STRA8, PRDM, STELLA, GDF9, SCP3, DDX4, and ZP2) in the 2 nd week of culture, suggesting the possibility of MenSCs differentiating into oocyte-like cells ( 24 ). To demonstrate the stem cell properties of MenSCs, studies have been conducted to test their pluripotency by culturing them with various types of differentiation media.…”

Section: Differentiation Of Menscsmentioning

confidence: 99%

Therapeutic Potential of Menstrual Blood-Derived Stem Cell Transplantation for Intrauterine Adhesions

Han

Yun

2022

Front. Surg.

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An increasing number of women experience intrauterine adhesion as a result of intrauterine operations, such as induced abortion, which can cause infertility, recurrent abortion and amenorrhea. Although some strategies have been applied clinically, such as hysteroscopy adhesiolysis of intrauterine adhesions, the results have not been promising. As regenerative medicine develops, research on menstrual blood-derived stem cell transplantation is increasing due to the properties of these cells, including self-renewal, differentiation, angiogenesis, anti-inflammation and immunomodulation. As a result, menstrual blood-derived stem cells may be an ideal cell source for the treatment of intrauterine adhesion. Excitingly, it has been reported that autologous menstrual blood stem cells could recovery injured endometrium and improve infertility in patients with refractory intrauterine adhesion. In this review, we discuss the possible potential of menstrual blood-derived stem cell transplantation for intrauterine adhesion, including the antifibrosis, angiogenesis, anti-inflammation and immunoregulation properties of the cells, which brings hopes for clinical therapy.

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Evaluating differentiation potential of the human menstrual blood-derived stem cells from infertile women into oocyte-like cells

Cited by 8 publications

References 41 publications

Menstrual Blood-Derived Endometrial Stem Cells’ Impact for the Treatment Perspective of Female Infertility

Menstrual Blood-Derived Endometrial Stem Cells’ Impact for the Treatment Perspective of Female Infertility

The Emerging Role of Menstrual-Blood-Derived Stem Cells in Endometriosis

Therapeutic Potential of Menstrual Blood-Derived Stem Cell Transplantation for Intrauterine Adhesions

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