Evaluating cell migration in vitro by the method based on cell patterning within microfluidic channels

Wang, Yan; Chen, Zhenling; Xiao, Le; Du, Zhiyan; Han, Xiaoxi; Yu, Xiaodan; Lu, Yinhui

doi:10.1002/elps.201100349

Cited by 9 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using defined patterns, cells from tumor regions could later be harvested for the gene expression analysis. Such microfluidic systems were also used to quantify cell migration as affected by chemotherapy drugs or other treatments (Wang et al, 2012). Moving to 3D culture, microfabrication using stereolithography could encapsulate MDA-MB-231, MCF-7, and MCF-10A in gelatin methacrylate to serve as a tumor model (Peela et al, 2016).…”

Section: Substrate Surface Patternsmentioning

confidence: 99%

The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis

Riehl

Kim

Bouzid

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Cancer can disrupt the microenvironments and mechanical homeostatic actions in multiple scales from large tissue modification to altered cellular signaling pathway in mechanotransduction. In this review, we highlight recent progresses in breast cancer cell mechanobiology focusing on cell-microenvironment interaction and mechanical loading regulation of cells. First, the effects of microenvironmental cues on breast cancer cell progression and metastasis will be reviewed with respect to substrate stiffness, chemical/topographic substrate patterning, and 2D vs. 3D cultures. Then, the role of mechanical loading situations such as tensile stretch, compression, and flow-induced shear will be discussed in relation to breast cancer cell mechanobiology and metastasis prevention. Ultimately, the substrate microenvironment and mechanical signal will work together to control cancer cell progression and metastasis. The discussions on breast cancer cell responsiveness to mechanical signals, from static substrate and dynamic loading, and the mechanotransduction pathways involved will facilitate interdisciplinary knowledge transfer, enabling further insights into prognostic markers, mechanically mediated metastasis pathways for therapeutic targets, and model systems required to advance cancer mechanobiology.

show abstract

Section: Substrate Surface Patternsmentioning

confidence: 99%

The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis

Riehl

Kim

Bouzid

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Wang et al demonstrated a microfluidic wound healing assay, where cells are aligned using channels, reducing the sampling volume and processing time compared to traditional wound healing assays. 13 Tong et al on the other hand, assembled a microfluidic migration chamber at a single cell resolution for direct visualisation of chemotaxis. 14 Tay's and Whitesides' groups studied cell migration and other cell behaviour using microfluidic gradient generators and micropatterns.…”

Section: Introductionmentioning

confidence: 99%

Infection and immunity on a chip: a compartmentalised microfluidic platform to monitor immune cell behaviour in real time

et al. 2015

View full text Add to dashboard Cite

Cells respond to their environments and self-organise into multicellular assemblies with dedicated functions. The migratory and homing response of cells to soluble ligands can be studied by using different techniques, but for real time studies of complex multicellular self-organisation, novel and simpler systems are required. We fabricated a flexible open access microsystem and tested the design by studying cell recruitment from an immune cell reservoir towards an infectious compartment. The two compartments were connected by a network of bifurcated microchannels allowing diffusion of signalling molecules and migration of cells. Bacterial filters were incorporated in the design to prevent bacteria and activated cells from entering the network, permitting migration only from the recruitment reservoir. The fabricated microsystem allows real-time continuous monitoring of cellular decision-making based on biologically produced gradients of cytokines and chemokines. It is a valuable tool for studying cellular migration and self-organisation in relation to infections, autoimmunity, cancer, stem cell homing, and tissue and wound repair.

show abstract

“…For example, Pathak and Kumar 7 investigated the decoupled influence of the stiffness and the geometrical size of the confinement area on tumor cell migration speed by studying the morphology and migration of U373-MG human glioma cells in a polyacrylamide microchannel, which was fabricated with the aid of a PDMS mold. Wang et al 11 demonstrated a simple microfluidic device based on PDMS to form cell arrays for investigating the wound healing process. Wong et al [12][13][14] developed a new method to regulate cell growth by covering a PDMS mold with micropatterns on a polystyrene Petri dish in order to make the uncovered areas hydrophilic, while the covered parts remained hydrophobic, after treatment by oxygen plasma.…”

Section: Introductionmentioning

confidence: 99%

Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics

et al. 2014

View full text Add to dashboard Cite

The culturing of cancer cells on micropatterned substrates can provide insight into the factors of the extracellular environment that enable the control of cell growth. We report here a novel non-UV-based technique to quickly micropattern a poly-(ethylene) glycol diacrylate (PEGDA)-based hydrogel on top of modified glass substrates, which were then used to control the growth patterns of breast cancer cells. Previously, the fabrication of micropatterned substrates required relatively complicated steps, which made it impractical for researchers to rapidly and systematically investigate the effects of different cell growth patterns. The technique presented herein operates on the principle of optically-induced electrokinetics (OEKs) and uses computer-generated projection light patterns to dynamically pattern the hydrogel on a hydrogenated amorphous silicon (a-Si:H) thin-film, atop an indium tin oxide (ITO) glass substrate. This technique allows us to pattern lines, circles, pentagons, and more complex shapes in the hydrogel with line widths below 3 μm and thicknesses of up to 6 μm within 8 s by simply controlling the projected illumination pattern and applying an appropriate AC voltage between the two ITO glass substrates. After separating the glass substrates to expose the patterned hydrogel, we experimentally demonstrate that MCF-7 breast cancer cells will adhere to the bare a-Si:H surface, but not to the hydrogel patterned in various geometric shapes and sizes. Theoretical analysis and finite-element model simulations reveal that the dominant OEK forces in our technique are the dielectrophoresis (DEP) force and the electro-osmosis force, which enhance the photo-initiated cross-linking reaction in the hydrogel. Our preliminary cultures of breast cancer cells demonstrate that this reported technique could be applied to effectively confine the growth of cancer cells on a-Si:H surfaces and affect individual cell geometry during their growth.

show abstract

Evaluating cell migration in vitro by the method based on cell patterning within microfluidic channels

Cited by 9 publications

References 34 publications

The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis

The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis

Infection and immunity on a chip: a compartmentalised microfluidic platform to monitor immune cell behaviour in real time

Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics

Contact Info

Product

Resources

About