Types of stripes: Microfluidic techniques are combined with surface chemistry to pattern multiple types of cells on the same substrate to simulate three types of naturally occurring cell–cell interactions. PDMS=poly(dimethylsiloxane) stamp, FN=fibronectin to promote cell adhesion, SAM=self‐assembled monolayer of an applied alkanethiol that resists cell adhesion.
This report establishes an in vitro model on glass surfaces for patterning multiple types of cells to simulate cell-cell interactions in vivo. The model employs a microfluidic system and poly(ethylene glycol)-terminated oxysilane (PEG-oxysilane) to modify glass surfaces in order to resist cell adhesion. The system allows the selective confinement of different types of cells to realize complete confinement, partial confinement, and no confinement of three types of cells on glass surfaces. The model was applied to study intercellular interactions among human umbilical vein endothelial cells (HUVEC), PLA 801 C and PLA801 D cells.
A resorufin derivative with a DBS group (probe 1) was designed and investigated for the detection of acetylcholinesterase (AChE) and inhibitor screening. The new assay is based on cascade enzymatic and chemical reactions of ATC, AChE and probe 1, and it can be carried out in a dual-signal detection mode. Moreover, the results show that probe 1 can be used for cell fluorescence staining.
Energetic-radiation-induced dimerization reaction of fullerenes was found to be a simple and highly selective method for synthesis of C2m-X-C2n (m = n or m not equal n) type molecules without formation of other products. Utilizing the new method, C70-C-C70, C60-C-C70, C60-C-C60, and C70-O-C70 were prepared and characterized. The method is capable of synthesizing new C2m-X-C2n molecules by introducing X (different atoms) into the reaction system. Energetic radiation created reactive sites for covalently bonded bridges between fullerene molecules originally only weakly bound by van der Waals force. This observation may open a new subject and practicable approach for polymer sciences of fullerenes.
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