Evaluación de la respuesta de isotipos de inmunoglobulina especifica a Leishmania en leishmaniasis tegumentaria Americana

Abstract

Leishmania-specific immunoglobulin subclass response was evaluated in 133 patients infected with Leishmania braziliensis. The indirect immunofluorescent antibody test (IFAT) was employed with amastigotes of L. mexicana amazonensis as antigen. Among the 133 sera obtained at consultation for diagnosis of active lesions, IgM was detected in 54 following absorption with Staphylococcus aureus Cowan strain I, and in 5 sera prior to absorption. IgM reactive with Leishmania antigen was only found in sera from patients whose lesions had evolved over the past two months or less. Leishmania-specific IgG was detected in all sera prior to absorption. Sera obtained at the time of recurrence or after complete healing of lesions presented only specific IgG. The combined use of the Montenegro skin test and specific IgM increased the sensitivity of immunodiagnostic methods in patients with lesions of less than 2 months duration. Normal control volunteers were negative for specific IgM and unreactive to Montenegro skin testing. Among 16 patients with non-leishmanial lesions, 3 with sporotrichosis showed IgG reactive with Leishmania; none, including 4 with lesions of less than two months duration, showed specific IgM. We conclude that in patients infected with L. braziliensis the presence of specific IgG and IgM is associated with the time of lesion evolution and the primary or recurrent nature of the lesions. In addition, the combined use of IgM titer and Montenegro reactivity is of potential utility in the diagnosis of early lesions.

Cutaneous scars in American tegumentary leishmaniasis patients: a site of Leishmania (Viannia) braziliensis persistence and viability eleven years after antimonial therapy and clinical cure.

“…30,31,33,34 The clinical value of positive serology in predicting the ATL recurrence in patients living in endemic areas is not established. 24,35 In the two cases presented, the IIF result was nonreactive despite positive cultures. The IIF results were confirmed by a researcher from another laboratory (Souza WJS, Instituto Oswaldo Cruz).…”

Control of rural house infestation by Triatoma infestans in the Bolivian Chaco using a microencapsulated insecticide formulation

“…The cost of the vector control intervention using the microencapsulated formulation is about 120 US dollars, for an average house with 300–320 square meters. Considering the longer lasting effect, the figure is below the cost of the standard intervention using pyrethroid insecticides, that ranges between 25 and 65 U$ per house [ 42 , 43 ] respectively. Inflation adjusted dollars to a 1999 common base give 85.8, 35.2, 67.5 (calculated through https://cps.ipums.org/cps/cpi99.shtml ) for the microencapsulated formulation, lower and upper extremes of pyrethroid-based intervention, respectively.…”

Host humoral immune response to Leishmania lipid‐binding protein

“…During the development of leishmaniasis the humoral immune response is characterized by an exaggerated reaction accompanied by the production of non‐protector polyclonal globulins, which occurs as a consequence of non‐specific polyclonal activation, just as has been described in other parasitoses (40). This type of response, producing high levels of IgG‐type immunoglobulins, is characteristic of both visceral and diffuse cutaneous leishmaniasis (41,42), whereas in other types of leishmaniasis, such as the cutaneous and mucose forms, lower antibody titres are found (43,44).…”