Etiology and histopathological alterations in some body organs of juvenile rainbow trout Oncorhynchus mykiss (Walbaum, 1792) at nitrite poisoning

Grynevych, Nataliia; Sliusarenko, A.; Dyman, Tetyana; Sliusarenko, S.; Gutyj, B. V.; Kukhtyn, Mykola; Hunchak, V.; Kushnir, В. І.

doi:10.15421/2018_228

Cited by 24 publications

(26 citation statements)

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“…Тому згідно з Регламентом ЄС 37/2010 "Про фармакологічно активні речовини та їх класифікацію відносно максимально допустимих кількостей в харчових продуктах тваринного походження" у рибі допускається певна кількість антибактеріальних препаратів. Разом із тим виробники не завжди дотримуються терміну каренції антибіотиків у рибі після останнього застосування, як наслідок цього у м'язах риби виявляють кількість цих препаратів, яка перевищує МДР (Samanidou and Evaggelopoulou, 2007;Akinbowale et al, 2007;Rico et al, 2012;Bayer et al, 2017;Grynevych et al, 2018). Залишки антибактеріальних препаратів у харчових продуктах негативно впливають на організм споживачів, спричиняючи різні алергічні стани та порушують мікробіоценоз травного тракту (Bhowmick et al, 2008;Grigorakis and Rigos, 2011;Kukhtyn et al, 2017;Kozlovska et al, 2018).…”

Section: вступunclassified

Toxic and biological evaluation of frozen fish meat in the presence of antibi-otic residues

Malimon¹,

Kukhtyn

Perkiy³

2018

SMLNUVMB

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Nowadays antimicrobial drugs of different pharmacologic classes are widely used in aquaculture all over the world. Therefore, in accordance with EU Regulation 37/2010 “On pharmacologically active substances and their classification in regard to maximum allowable amounts in nourishing food of animal origin”, there are certain amount of antimicrobial drugs acceptable in fish. The aim of the work was to determine toxico-biological assessment of flesh of frozen fish where there are residues of antimicrobial drugs on the culture of infusorias Tetrachymena pyriformis. The availability of residual amounts of antimicrobial drugs was carried out by screening method for antibiotics in products of animal origin РХ/МС/МС and microbiological method. Toxico-biological assessment of flesh of frozen fish was determined in accordance with “Methodological recommendations on toxico-biological assessment of flesh, flesh products and milk with infusoria Tetrachymena pyriformis”. It was found that samples of frozen fish containing residues of antimicrobial drugs of aminoglycosides, penicillic and fluoroquinolonic class were not toxic for Tetrachymena pyriformis cultures. Since infusorias were active, natural movement – progressive and circular, inhibition of growth or change of body shape were not showed compared to infusorias within control group. It was shown that in the fish flesh with residues of tetracycline antibiotics, there was observed the inhibition of growth and decreased activity and mobility of infusorias Tetrachymena pyriformis up to 20% compared to control without antibiotics. However, unnatural movements (affected or oscillatory) and pathological changes in the shape of infusoria cultures were not detected. This flesh fish was characterized as moderate toxic. It was stated that relative biological value of frozen fish flesh with antibiotic residues of aminoglycosides, and fluoroquinolonic class was lower by 0,27–0,73% compared to relative biological value of control fish flesh without antibiotics. At the same time relative biological value of fish flesh with the presence of residues of tetracycline antibiotics was by 4.12% lower compared to control fish flesh. Consequently, conducted studies indicate the need to control the residues of antimicrobial drugs in frozen fish which is imported to Ukraine. In case of detection the exceeding permissible amount of antibiotic residues in frozen fish it should be discarded, as it exhibits a moderately toxic effect.

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Section: вступunclassified

Toxic and biological evaluation of frozen fish meat in the presence of antibi-otic residues

Malimon¹,

Kukhtyn

Perkiy³

2018

SMLNUVMB

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“…Heavy metal salts, particularly Cadmium and Lead, which are highly toxic and can affect living organisms, even at low concentrations, are among the leading chemical pollutants (Ali et al, 1986;Hutyi, 2015;Gutyj et al, 2018;2019). The frequency and severity of ecologically dependent diseases resulting from anthropogenic pollution of the biosphere indicates the relevance of this problem Grynevych et al, 2018;Slivinska et al, 2019). Heavy metals are priority pollutants of atmospheric air, reservoir water and soil globally and regionally.…”

Section: Introductionmentioning

confidence: 99%

Influence of heavy metals on hematologic parameters, body weight gain and organ weight in rats

Lopotych¹,

Panas²,

Datsko³

et al. 2020

Ukr J Ecol

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The aim of the study was to determine the effect of Cadmium and Lead salts on body weight gain and rat body weights and hematological parameters. The experiments were conducted in male rats of the Wistar line, weighing 200–220 g, of which four groups of animals were formed: 1) A control group – they injected drinking water through a metal probe in a volume equivalent to the volume of an aqueous solution of Cd2+ salts and Pb2+; 2) Experimental group 1 – animals were administered a 0.029% aqueous solution of cadmium chloride at a dose of 4.0 mg kg-1; 3) Experimental group 2 – animals were administered 16.6% aqueous lead acetate solution at a dose of 200 mg kg-1; 4) Experimental group 3 – animals were administered 16.6% aqueous lead acetate solution at a dose of 100 mg kg-1 and 0.029% aqueous cadmium chloride solution at a dose of 2.0 mg kg-1. Throughout the experiment, rats were kept in a balanced diet containing all the necessary components, and animals were given drinking water, without restriction, from 0.2 liter glass bowls. On the basis of the conducted researches it is established that at loading of an organism of rats by cadmium and Lead salts in rats the weight gain in comparison with intact animals decreased. Reduction of live weight gain in rats by heavy metal intoxication was accompanied by hypo- and hypertrophy of the internal organs. These changes are related to the cumulative and sorption capacity of these metal ions, which contribute to the development of endogenous intoxication of rats in the experimental groups. Chronic lead-cadmium toxicosis in rats was accompanied by erythrocytopenia and leukopenia, as well as a decrease in hemoglobin with a simultaneous increase in erythrocyte volume and average erythrocyte hemoglobin content.

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“…Over the past decades, an increase in the content of this metal in the soils of Ukraine and other countries has been accompanied by the accumulation of Cd 2+ in agricultural products and feed, an increase in the threat to human and animal health. Hradovych et al, 2016;Grushanska, 2017;Grynevych et al, 2018). Cadmium and its compounds belong to the immunotoxicant, which cause disruptions in the functioning of the body's immune system, reduce resistance to infections, contribute to the formation of allergic, autoimmune and oncological pathologies (Ali et al, 1986;Salvatori et al, 2004;El-Refaiy and Eissa, 2012;Peng et al, 2015;.…”

Section: Introductionmentioning

confidence: 99%

The effect of cadmium loading on protein synthesis function and functional state of laying hens’ liver

Gutyj¹,

Ostapiuk²,

Kachmar³

et al. 2019

Ukr J Ecol

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In the article are considered issues related to the study of the effect of cadmium on the body of birds, in particular laying hens. The effect of cadmium sulfate at doses of 2.0 and 4.0 mg/kg body weight on protein synthesis function and functional state of the liver were investigated of cross-laying Hysex white hens, of 78 weeks old. Three groups of chickens were formed: two experimental and one control groups. The laying hens of the experimental groups were subjected to a cadmium load. Laying chickens of the first experimental group were given cadmium sulfate at a dose of 2.0 mg/kg body weight. Laying chickens of the second experimental group were given cadmium sulfate at a dose of 4.0 mg/kg body weight. Laying chickens in the control group were in the normal diet without cadmium. Increased activity of aminotransferases, in particular alanine and aspartate aminotransferases in their serum of hens, was observed during cadmium binge drinking in doses of 2.0 and 4.0 mg/kg body weight, respectively. These changes indicate a violation of the functional state of the liver of laying hens under conditions of cadmium load. In the study of protein synthesis of the liver of laying hens under conditions of cadmium loading, it was found that cadmium sulfate in the above doses contributed to a significant decrease in the level of total protein by 14, 21 and 30 days of the experiment. The reduction of total protein in the blood of laying hens was due to a decrease in the level of albumin, which in the second experimental group, respectively, decreased to 28.16 ± 0.75%, whereas in the first – 29.62 ± 0.98%. In the study of the level of globulins, it is possible to increase this indicator in both experimental groups of poultry for 21 days. Drinking with water cadmium sulfate at a dose of 4.0 mg/kg body weight was associated with a more likely decrease in total protein and albumin than drinking cadmium sulfate at a lower dose.

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Etiology and histopathological alterations in some body organs of juvenile rainbow trout Oncorhynchus mykiss (Walbaum, 1792) at nitrite poisoning

Cited by 24 publications

References 0 publications

Toxic and biological evaluation of frozen fish meat in the presence of antibi-otic residues

Toxic and biological evaluation of frozen fish meat in the presence of antibi-otic residues

Influence of heavy metals on hematologic parameters, body weight gain and organ weight in rats

The effect of cadmium loading on protein synthesis function and functional state of laying hens’ liver

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