Toxic and biological evaluation of frozen fish meat in the presence of antibi-otic residues

Abstract

Nowadays antimicrobial drugs of different pharmacologic classes are widely used in aquaculture all over the world. Therefore, in accordance with EU Regulation 37/2010 “On pharmacologically active substances and their classification in regard to maximum allowable amounts in nourishing food of animal origin”, there are certain amount of antimicrobial drugs acceptable in fish. The aim of the work was to determine toxico-biological assessment of flesh of frozen fish where there are residues of antimicrobial drugs on the culture of infusorias Tetrachymena pyriformis. The availability of residual amounts of antimicrobial drugs was carried out by screening method for antibiotics in products of animal origin РХ/МС/МС and microbiological method. Toxico-biological assessment of flesh of frozen fish was determined in accordance with “Methodological recommendations on toxico-biological assessment of flesh, flesh products and milk with infusoria Tetrachymena pyriformis”. It was found that samples of frozen fish containing residues of antimicrobial drugs of aminoglycosides, penicillic and fluoroquinolonic class were not toxic for Tetrachymena pyriformis cultures. Since infusorias were active, natural movement – progressive and circular, inhibition of growth or change of body shape were not showed compared to infusorias within control group. It was shown that in the fish flesh with residues of tetracycline antibiotics, there was observed the inhibition of growth and decreased activity and mobility of infusorias Tetrachymena pyriformis up to 20% compared to control without antibiotics. However, unnatural movements (affected or oscillatory) and pathological changes in the shape of infusoria cultures were not detected. This flesh fish was characterized as moderate toxic. It was stated that relative biological value of frozen fish flesh with antibiotic residues of aminoglycosides, and fluoroquinolonic class was lower by 0,27–0,73% compared to relative biological value of control fish flesh without antibiotics. At the same time relative biological value of fish flesh with the presence of residues of tetracycline antibiotics was by 4.12% lower compared to control fish flesh. Consequently, conducted studies indicate the need to control the residues of antimicrobial drugs in frozen fish which is imported to Ukraine. In case of detection the exceeding permissible amount of antibiotic residues in frozen fish it should be discarded, as it exhibits a moderately toxic effect.

Toxicological characteristics of raw milk with different contents of 17β-estradiol

“…Отже, дослідження з визначення безпосереднього гострого та хронічного токсичного впливу естрогенних гормонів, зокрема 17β-естрадіолу, наявного у молоці-сировині, на живих тест-об'єктах та встановлення його біологічної цінності дозволяє метод з використанням одноклітинних організмів, зокрема інфузорій Tetrachymena pyriformis (Malimon et al, 2018;Kozhyn et al, 2021).…”