Etiological agents of bacterial sepsis in a newly constructed medical center in Saint Petersburg, Russia

Barantsevich, E. P.; Barantsevich, N.E.; Rybkova, N; Churkina, I. V.; Pestova, N; Карпенко, М. А.

doi:10.1186/cc10414

Cited by 7 publications

(7 citation statements)

References 6 publications

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“…This finding is in agreement with most published studies (16, 17, 20). considering this issue when dealing with sepsis may help in administration of proper antibiotics and prevention of treatment failure.…”

Section: Discussionsupporting

confidence: 94%

“…In this study, we also found that approximately 60% of admitted patients had been under unnecessary treatments. Similar reports of sepsis over-diagnosis and unnecessary antibiotic usage in hospitalized patients with primary diagnosis of sepsis are available ( 5 , 11 , 16 - 19 ). Treatment of sepsis in the ICU as in the internal ward was inappropriate, whereas in the infectious diseases ward, the treatment was mostly appropriate (12/61, 19.7% vs. 61/98, 62.2%).…”

Section: Discussionsupporting

confidence: 55%

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Bacterial Sepsis: Challenges of Diagnosis and Treatment in a Teaching Hospital Southwest of Iran

Alavi

Sharifi

Eghtesad

2014

Jundishapur J Microbiol

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Background:Management of bacterial sepsis as a common cause of hospitalization and a life threatening clinical syndrome is a challenge. In previous studies, incorrect diagnosis of sepsis and unnecessary treatment have been frequently reported.Objectives:The aim of this study was to evaluate the diagnosis and treatment of cases with a primary diagnosis of sepsis.Patients and Methods:Of 410 medical files of patients with primary diagnosis of bacterial sepsis, 187 fulfilled our criteria and were enrolled in the study. The study was conducted in Razi Hospital of Ahvaz, southwest Iran, from 2009 to 2011. Data included demographic characteristics, underlying disease, clinical symptoms, laboratory and imaging findings, administrated antibacterial drugs, and nurses and doctors-analyzed notes. For evaluation of the diagnosis, patients were divided to two groups, sepsis group and pseudosepsis group, and for evaluation of the treatment, patients were categorized in appropriate and inappropriate treatment groups and compared using SSPS software version 16 by chi-square and fisher exact tests. P-values less than 0.05 were considered significant.Results:Out of 187 cases, 61 were in the intensive care unit (ICU), 98 in the infectious disease ward, and 28 in the internal medicine ward. Correct diagnosis of sepsis in the ICU, internal and infectious diseases wards were made in 16 (26.2%), 4 (14.3%) and 71 (72.4%) cases, respectively. Appropriate treatments for sepsis in the ICU, internal and infectious wards were applied in 12 (19.7%), 3 (10.7%) and 61 (78.2%) cases, respectively. Ninety-one patients (48.6%) were diagnosed correctly (true sepsis) and 76 (40.6%) were treated with proper regimes.Conclusions:Inappropriate and unnecessary use of antibiotics by patients with preliminary diagnosis of sepsis in our hospital, similar to other parts of the world, was high.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 55%

Bacterial Sepsis: Challenges of Diagnosis and Treatment in a Teaching Hospital Southwest of Iran

Alavi

Sharifi

Eghtesad

2014

Jundishapur J Microbiol

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“…While the WHO estimates approximately 63,000 annual deaths are due to E. coli infections, the added consequence of 5 million years of life lost (YLLS) and 5 million disability adjusted life years (DALYS) further compounds the suffering caused by this pathogenic contaminant 2 . In addition to GI tract infections, E. coli is responsible for 8.9% of sepsis cases, 29% of early onset neonatal sepsis cases and the majority of urinary tract infections 3 , 4 . Generic E. coli species which consist of both pathogens and non-pathogens, are ubiquitous in mammalian feces 5 – 9 .…”

Section: Introductionmentioning

confidence: 99%

A phage-based assay for the rapid, quantitative, and single CFU visualization of E. coli (ECOR #13) in drinking water

Hinkley

Singh

Garing

et al. 2018

Sci Rep

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Drinking water standards in the United States mandate a zero tolerance of generic E. coli in 100 mL of water. The presence of E. coli in drinking water indicates that favorable environmental conditions exist that could have resulted in pathogen contamination. Therefore, the rapid and specific enumeration of E. coli in contaminated drinking water is critical to mitigate significant risks to public health. To meet this challenge, we developed a bacteriophage-based membrane filtration assay that employs novel fusion reporter enzymes to fully quantify E. coli in less than half the time required for traditional enrichment assays. A luciferase and an alkaline phosphatase, both specifically engineered for increased enzymatic activity, were selected as reporter probes due to their strong signal, small size, and low background. The genes for the reporter enzymes were fused to genes for carbohydrate binding modules specific to cellulose. These constructs were then inserted into the E. coli-specific phage T7 which were used to infect E. coli trapped on a cellulose filter. During the infection, the reporters were expressed and released from the bacterial cells following the lytic infection cycle. The binding modules facilitated the immobilization of the reporter probes on the cellulose filter in proximity to the lysed cells. Following substrate addition, the location and quantification of E. coli cells could then be determined visually or using bioluminescence imaging for the alkaline phosphatase and luciferase reporters, respectively. As a result, a detection assay capable of quantitatively detecting E. coli in drinking water with similar results to established methods, but less than half the assay time was developed.

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“…According to the WHO estimates approximately 63,000 annual deaths are due to E. coli infections [1]. Beside gastrointestinal tract infections, E. coli is responsible for 8.9% of sepsis cases, 29% of early onset neonatal sepsis cases and the majority of urinary tract infections [2].…”

Section: Introductionmentioning

confidence: 99%

Isolation of bacteriophages from untreated sewage water against multi-drug resistant E.coli - An initiative to fight against drug resistance

Qamar

Owais

Chauhan

et al. 2019

Preprint

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Background: Pathogenic Escherichia coli, common drinking water contaminant, cause a large number of morbidity and mortality worldwide. According to the WHO estimates approximately 63,000 annual deaths are due to E. coli infections. Due to selective pressure on coliforms, resistant microbial strains are produced that threatens modern medicine where common infections could become more deadly. So, there is an urgent need to develop alternative anti-microbial to replace existing antibiotics for treating a broad spectrum of bacterial diseases. This revived the interest of scientists in phages as an alternative therapy. Phage therapy is defined as a therapeutic use of bacteriophages (natural predators of bacteria) for treating bacterial infections. In the present study pure phage strain was isolated from the untreated sewage water sample and subjected to 10 fold dilution following double agar layer assay to determine phage titer against multi-drug resistant E.coli following host range analysis and stability testing at varying temperature and pH. Results: Sewage water contains a vast variety of different sizes bacteriophages with clear to diffused boundaries. The pure plaque isolated after repeated plating showed that it was highly specific against tested E. coli strain and could not lyse strains from other species. The titer was calculated to be 109 PFU/ml that remained unchanged at 4°C, 37°C and 50°C temperature. However, at higher pH range phage viability decreases. Conclusions: In future, it would be expected that the isolated bacteriophages could be characterized and used as a therapeutic potential against multi-drug resistant E.coli that not only attenuate superbug spread but could also replace antibiotics. Beside, isolated phages would be utilized as a bio-component in biosensor development against food borne pathogenic bacteria.

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Etiological agents of bacterial sepsis in a newly constructed medical center in Saint Petersburg, Russia

Cited by 7 publications

References 6 publications

Bacterial Sepsis: Challenges of Diagnosis and Treatment in a Teaching Hospital Southwest of Iran

Bacterial Sepsis: Challenges of Diagnosis and Treatment in a Teaching Hospital Southwest of Iran

A phage-based assay for the rapid, quantitative, and single CFU visualization of E. coli (ECOR #13) in drinking water

Isolation of bacteriophages from untreated sewage water against multi-drug resistant E.coli - An initiative to fight against drug resistance

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