Background: In the emerging field of nanotechnology, copper oxide (CuO) nanomaterials are considered to be one of the most important transition metal oxides owing to its fascinating properties. Its synthesis from green chemistry principles is gaining importance as next-generation antibiotics due to its simplicity, eco-friendliness, and cost-effectiveness. In the present study, CuO nanorods (CuO NRs) were synthesized from the aqueous fruit extract of Momordica charantia and characterized using different analytical techniques. Further, the biomedical therapeutic potential was evaluated against multi-drug resistant microbial strains. Materials and Methods: To synthesize CuO NRs, 0.1M of CuSO 4 .5H 2 O solution was added to aqueous extract of Momordica charantia in a 1:3 (v/v) ratio (pH=11) and heated at 50°C followed by washing and drying. The synthesized CuO NRs were subjected to characterization using different analytical techniques such as UV visible spectroscopy, zeta sizer equipped with zeta potential, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) equipped with energydispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). Further, the application as a biomedical therapeutic potential was evaluated in vitro using well diffusion method against eleven multidrug-resistant clinical bacterial strains, a fungus-Trichophyton rubrum and in ovo against the R 2 B virus using haemagglutination (HA) test. Results: Characterization was preliminarily done by the spectral study that confirms the absorbance band at 245nm. FTIR analysis at 628 cm −1 peak identified copper oxide vibration. SEM analysis revealed agglomerated particle clusters. However, with TEM clear nanorods of average diameter of 61.48 ± 2 nm were observed. EDAX confirmed CuO formation while XRD showed a typical monoclinic structure with 6 nm crystallite size. Biological screening of CuO NRs showed significant results against both in vitro and in ovo methods. Significant inhibitory activity (p<0.0001) was noted against most of the resistant human pathogenic strains including both Gram-positive and Gram-negative bacteria. The highest efficacy was observed against Bacillus cereus with a 31.66 mm zone of inhibition. Besides, the therapeutic potential of CuO NRs against Corynebacterium xerosis, Streptococcus viridians and R 2 B strain of Newcastle disease is reported for the first time.Conclusion: Based on the present results, it could be expected that green synthesized CuO NRs would find potential applications in the field of nanomedicine.
Background: Pathogenic Escherichia coli, common drinking water contaminant, cause a large number of morbidity and mortality worldwide. According to the WHO estimates approximately 63,000 annual deaths are due to E. coli infections. Due to selective pressure on coliforms, resistant microbial strains are produced that threatens modern medicine where common infections could become more deadly. So, there is an urgent need to develop alternative anti-microbial to replace existing antibiotics for treating a broad spectrum of bacterial diseases. This revived the interest of scientists in phages as an alternative therapy. Phage therapy is defined as a therapeutic use of bacteriophages (natural predators of bacteria) for treating bacterial infections. In the present study pure phage strain was isolated from the untreated sewage water sample and subjected to 10 fold dilution following double agar layer assay to determine phage titer against multi-drug resistant E.coli following host range analysis and stability testing at varying temperature and pH. Results: Sewage water contains a vast variety of different sizes bacteriophages with clear to diffused boundaries. The pure plaque isolated after repeated plating showed that it was highly specific against tested E. coli strain and could not lyse strains from other species. The titer was calculated to be 109 PFU/ml that remained unchanged at 4°C, 37°C and 50°C temperature. However, at higher pH range phage viability decreases. Conclusions: In future, it would be expected that the isolated bacteriophages could be characterized and used as a therapeutic potential against multi-drug resistant E.coli that not only attenuate superbug spread but could also replace antibiotics. Beside, isolated phages would be utilized as a bio-component in biosensor development against food borne pathogenic bacteria.
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