Ethylene Production by Slices of Green Banana Fruit and Potato Tuber Tissue During The Development of Induced Respiration

McGlasson, W. B.

doi:10.1071/bi9690489

Cited by 74 publications

(39 citation statements)

References 4 publications

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“…Only with cyanide was the normal level of ethylene output regained, and it was only in this case that ripening appeared normal. The inhibition of the induced ethylene peak would appear to be a further indication of the special role ethylene may play in regulating metabolism within fruit tissues (McGlasson 1969}. Large increases in ethylene production by citrus fruits have been measured following treatment with cycloheximide (Cooper, Rasmussen, and Hutchison 1969).…”

Section: Table 4 Sugar Levels In Slices Treated With Potassium Cyanidementioning

confidence: 99%

“…and incubated at 20°C as described by Palmer and McGlasson (1969). Respiration (C02 production) was measured colorimetrically or with an infrared gas analyser (Palmer and McGlasson 1969) and ethylene was measured by gas chromatography (McGlasson 1969). Inhibitors were vacuum-infiltrated (1 min, 26 in.…”

Section: (A) Preparation and Handling Of Slicesmentioning

confidence: 99%

“…Studies of ethylene production (McGlasson 1969), of carbon metabolism (Palmer and McGlasson 1969;Brady et al 1970a;McGlasson et al 1971), of protein biosynthesis (Brady et al 1970b), and of the effects of plant growth substances (Vendrell 1969(Vendrell , 1970Wade and Brady 1971) have been made using these slices.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic Studies with Banana Fruit Slices II. Effects of Inhibitors on Respiration, Ethylene Production, and Ripening

McGlasson¹,

Palmer²,

Vendrell³

et al. 1971

Aust. Jnl. Of Bio. Sci.

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Transverse slices of green banana fruit were vacuum· infiltrated with aqueous solutions of 24 potential inhibitors of protein synthesis, respiration, or ethylene production. The effects of these compounds were examined in the absence or presence of 10 p.p.m. ethylene. Of the compounds which produced marked effects mono· fiuoroacetate, 4·hydroxy·2·oxoglutarate (HKG), KCN, and cycloheximide were examined in more detail.Monofiuoroacetate (1 and 2 X 10-2M) and cycloheximide (1 and 2I-'g/ml) almost eliminated the peaks of induced respiration and ethylene production, almost eliminated the respiratory response to added ethylene, and prevented normal ripening. Ethylene production by slices treated with the inhibitors rose within a few hours after treatment to steady levels considerably above the preclimacteric rates of untreated slices, possibly as a result of cell damage. KCN at 5 X 10-2M caused breakdown of the tissue. At 1 and 2·5 X 10-2M, KCN almost eliminated the peak of induced ethylene production, but only delayed the peak of induced respira. tion and retarded ripening in both ethylene. treated and ungassed slices. Before the resumption of a normal ripening pattern in slices treated with KCN ethylene pro· duction declined to normal preclimacteric rates. HKG at 3 X lO-2M eliminated the induced rise in ethylene production, delayed the peak of induced respiration, and reduced respiration in ethylene-treated slices but did not affect ripening. There is evidence that banana tissue may metabolize HKG and KCN.

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Section: Table 4 Sugar Levels In Slices Treated With Potassium Cyanidementioning

confidence: 99%

Section: (A) Preparation and Handling Of Slicesmentioning

confidence: 99%

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Metabolic Studies with Banana Fruit Slices II. Effects of Inhibitors on Respiration, Ethylene Production, and Ripening

McGlasson¹,

Palmer²,

Vendrell³

et al. 1971

Aust. Jnl. Of Bio. Sci.

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“…The physiology of this latter response has been much studied, and the mechanism of the increase is still the subject of controversy. Laties (8) (9), and it is reasonable to postulate that the subsequent respiration increase may be induced by this endogenous ethylene. The data which led Laties (8) …”

Section: Effect Of Ethylene Treatment On the Respiration Of Potatomentioning

confidence: 99%

Effects of Ethylene on Potato Tuber Respiration

Reid

Pratt

1972

Plant Physiol.

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Treatment of potato tubers (Solanum tuberosum L.) with ethylene gas causes a rapid rise in their respiration rate, reaching 5 to 10 times the rate of untreated tubers over 30 hours of treatment and then falling slowly. The response shows a lag of 8 hours, and more than 24 hours of exposure is required for maximum effect; the temperature optimum is near 25 C. In sensitivity to low concentrations and dependence on temperature, the phenomenon is similar to the effect of ethylene on the respiration of climacteric and nonclimacteric fruits. Treated potato tubers returned to air recover their sensitivity to ethylene more slowly than do nonclimacteric fruits (e.g., mature green oranges). It is proposed that the respiratory rise characteristic of ripening in climacteric fruits and of the wound response in plant tissues is induced by a rise in endogenous tissue ethylene.Ethylene is a plant hormone having diverse effects on a wide range of plant tissues. In spite of its apparent importance as a regulator of plant growth, the site and mechanisms of its actions have not yet been elucidated (10, 12). We recently described the remarkable effect of this substance on the respiration of intact potato tubers (14) and suggested the possible value of the phenomenon as a simple system for the study of the mode of action of ethylene. We report here a more detailed study of the respiratory response of potato tubers to ethylene gas and discuss the mechanisms that may be involved. MATERIALS AND METHODSMature (100 day) potato tubers (Solanum tuberosum L., cv. White Rose) were dug from the field, washed, and stored at 20 C until needed; all experiments except the temperature trial were conducted at this temperature. Commercially obtained tubers were found to be unsatisfactory because previous cold storage and careless handling may have had pronounced effects on their physiology. Mature green sweet oranges (Citrus sinensis Osbeck, seedling) were obtained from a local garden.Respiration measurements were made by placing individual specimens in 500-ml jars ventilated with a stream of air flowing at about 1.5 liter per hr and measuring the CO, content of the effluent air by gas chromatography. Ethylene was in-'This work was supported in part by research grant FD-00071 from the United States Public Health Service.2 On leave from Plant Diseases Division, D.S.I.R., Auckland, New Zealand. troduced into gas streams from a pressure cylinder through a reduction valve and appropriate capillary flowmeters, and the final concentration was verified with a gas chromatograph.When it was necessary to measure respiration in a static system, individual specimens were placed in 9-liter jars, and ethylene in appropriate amounts was added through a rubber septum. Samples of the jar atmosphere were taken through the septum for determination of the CO2 content. Changes in concentration resulting from sample removal were insignificant, and the jar volume was such that the concentration of CO2 seldom rose above 0.5%. RESULTS Effect of Ethylene Treatment on ...

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“…The latter rise has been termed "induced" respiration (Laties 1963;ap Rees 1966). In addition, when the bananas are cut, ethylene production immediately increases about lO-fold and then rises to a peak after 6-8 hr (McGlasson 1969). Many types of plant tissue show a similar pattern of induced respiration after cutting (ap Rees 1966;Laties 1967), and up to the present a period of induced ethylene synthesis has been observed only in fruit (Galliard et al 1968b;McGlasson 1969;Lee, McGlasson, and Edwards 1970).…”

Section: Introduotionmentioning

confidence: 93%

Metabolic Studies with Banana Fruit Slices I. Changes in the Incorporation of 14c Labelled Compounds in Response to Cutting

McGlasson¹,

Vendrell²,

Brady³

1971

Aust. Jnl. Of Bio. Sci.

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SummaryTransverse slices of green banana fruit were vacuum-infiltrated with aqueous solutions of 14C-labelled metabolites and the non-metabolite a-methyl-D-glucoside immediately after cutting, and at 17 hr after cutting-i.e. near the peak of "induced" respiration.The The rate of uptake of acetate and malonate is rapid in slices both at 0 and 17 hrafter cutting, wheras the rate of uptake of citrate and D-glucose is low at 0 hr but rapid at 17 hr after cutting. a-Methyl-D-glucoside is taken up at a low and similar rate at both times. The patterns of release of 14C02 from 14C-Iabelled glucose, acetate, and malonate show that the metabolic responses induced by cutting begin within 3 hr after cutting.

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Ethylene Production by Slices of Green Banana Fruit and Potato Tuber Tissue During The Development of Induced Respiration

Cited by 74 publications

References 4 publications

Metabolic Studies with Banana Fruit Slices II. Effects of Inhibitors on Respiration, Ethylene Production, and Ripening

Metabolic Studies with Banana Fruit Slices II. Effects of Inhibitors on Respiration, Ethylene Production, and Ripening

Effects of Ethylene on Potato Tuber Respiration

Metabolic Studies with Banana Fruit Slices I. Changes in the Incorporation of 14c Labelled Compounds in Response to Cutting

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