Ethanol Induced Oxidative Stress and Membrane Injury in Rat Erythrocytes

Sözmen, Eser Yıldırım; Tanyalçın, Tijen; Onat, Toner; Kutay, Fatma Z.; Erlaçin, S

doi:10.1515/cclm.1994.32.10.741

Cited by 27 publications

(22 citation statements)

References 20 publications

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“…As well as some beneficial effects, the ethanol exerts serious side-effects in the central nervous system (CNS), liver and other tissues. It is known that the ethanol increases the generation of reactive oxygen species and leads to oxidative damage in erythrocytes [24,25]. In fact, the ethanol causes several haemolytic disorders due to both direct and indirect effects [13].…”

Section: Resultsmentioning

confidence: 99%

The effect of ethanol on erythrocyte carbonic anhydrase isoenzymes activity: An in vitro and in vivo study

Çoban

Beydemır

Gülçın

et al. 2008

Journal of Enzyme Inhibition and Medicinal Chemistry

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The ethanol is a widely consumed as sedative-hypnotic drug throughout the world. In this study, the effects of ethanol were investigated on carbonic anhydrase (CA) enzyme activities both in vitro in human erythrocyte and in vivo in Sprague-Dawley rat erythrocyte. For in vitro study, the human carbonic anhydrase-I (HCA-I) and -II (HCA-II) are purified by Sepharose 4B -L-tyrosine-sulphanilamide affinity chromatography. In vivo CA enzyme activity was determined colorimetrically by using CO 2 -hydration method of Wilbur and Anderson. Rat blood samples were taken from each rat before and after the ethanol administration at different times (1 h, 3 h, and 5 h). Rat erythrocyte CA activity was significantly inhibited by pharmacological dosage of the ethanol (2 mL.kg 21) for up to 3 h ( p , 0.001) following intraperitoneally administration. The ethanol showed in vitro inhibitory effects on HCA-I and HCA-II hydratase activity, determined by colorimetrically using the CO 2 -hydratase method. The inhibitor concentrations causing up to 50% inhibition (IC 50 ) were 2.09 M for HCA-I (r 2 :0.9273) and 1.83 M for HCA-II (r 2 :9749). In conclusion, it was demonstrated that carbonic anhydrase enzyme in erythrocytes was significantly inhibited by the ethanol both in in vitro and in vivo.

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Section: Resultsmentioning

confidence: 99%

The effect of ethanol on erythrocyte carbonic anhydrase isoenzymes activity: An in vitro and in vivo study

Çoban

Beydemır

Gülçın

et al. 2008

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…This is in consonance with the findings that alcohol and smoking causes membrane deformity. 12 Similarly excessive alcohol intake causes membrane lesion and leakage of small potassium ion from the cells. The level of membrane lesion increases with increasing alcohol concentration leading to higher osmotic fragility (MCF).…”

Section: Discussionmentioning

confidence: 99%

“…There are evidence that alcohol and smoking causes membrane deformity and as a result a modification of the osmotic fragility in different cell types. 12 The study therefore aims at assessing the impact of alcohol on haemorrheological parameters and osmotic fragility of consumers.…”

Section: Introductionmentioning

confidence: 99%

Effect of alcohol consumption on haemorheology and osmotic fragility of subjects in Nnewi, South-eastern, Nigeria

O¹,

AmahObioma²,

Grace³

et al. 2017

Int. J. Sport Exc. Health Res.

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Background: Alcohol consumption has been known to have physiological, mental, psychological and haematological effects on consumers. Aim and Objectives: This is study was aimed at assessing the effects of alcohol consumption on haemorrheology and osmotic fragility of alcoholconsumers in Nnewi metropolis as well as the gender and age variations. Study Design: This is a case control study. Method: A total of 100 subjects comprising 50 alcohol consumers (35 males and 15 females) and 50 non-alcohol consumers (35 males and 15 females) were recruited. The study subjects were tested for Relative Whole blood viscosity (RWBV), Relative plasma viscosity (RPV), Relative serum viscosity (RSV) and Osmotic fragility (Median Corpuscular Fragility (MCF) after obtaining ethical approval and informed consent of the subjects. RSV, RWBV and RPV were carried out by a modification of the capillary method of Reid and Ugwu (1987). Osmotic fragility was estimated using the method described by Dacie and Lewis (2012). Statistical Package for Social Sciences (SPSS) version 20 software was used for statistical analysis. Results: The results showed a significant increase in the mean of RWBV, RPV, RSV and MCF of alcohol consumers when compared with non-alcohol consumers (P<0.05). Similarly, the mean values of RWBV of male alcohol consumers were significantly higher than that of female alcohol consumers. However, there was no significant difference in the mean values of RWBV, RPV, RSV and MCF of alcohol consumers based on different age group (P>0.05). Also the mean values of RPV, RSV and MCF between male and female alcohol consumers showed no significant difference. Conclusion: Alcohol consumption results in significant alterations in haemorrheology and osmotic fragility of consumers.

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“…After dilution in the required amount of tissue homogenates, TBARS levels were determined according to the protocol described by Sozmen et al [16], by incubation with MDA solution (0.12 M TBA in 15% TCA and 1% HCl) for 30 min at 95°C, and were calculated as nmol/mg protein using a 1,1,3,3,tetramethoxypropane standard curve while the hemolysate MDA levels were expressed as nmol/g Hb.…”

Section: Quantification Of Mdamentioning

confidence: 99%

The Effects of Different Intraabdominal Pressure Protocols in Laparoscopic Procedures on Oxidative Stress Markers and Morphology in Rat Ovaries

Biler¹,

Yücebilgin²,

Şendağ³

et al. 2014

Adv Clin Exp Med

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Background. To determine the effects of different intraabdominal pressure (IAP) on the ovaries in a laparoscopic rat model. Objectives. The aim of the study was to determine the effects on the ovaries of different intraabdominal pressures (IAP) in laparoscopic surgery in a rat model. Material and Methods. Thirty-two post-pubertal nonpregnant Sprague-Dawley rats were divided randomly into four groups. In the control group, no intraabdominal pressure (IAP) was applied. In Group Pp10 and Group Pp15, an IAP of 10 and 15 mm Hg, respectively, were applied by carbon dioxide insufflation for 60 min, and a 30-min desufflation was carried out. In Group IPp15, a 15 mm Hg IAP was applied for 10 min, and then CO 2 was desufflated for 10 min. After this ischemic preconditioning, IAP was established at 15 mm Hg for 60 min, after which CO 2 was desufflated for 30 min. Erythrocyte and ovarian tissue malondialdehyde (MDA) and histopathologic damage scores were evaluated. Results. In Groups Pp10 and Pp15, ovarian tissue MDA values were significantly increased compared to the control group. In Groups Pp10 and Pp15, erythrocyte MDA values were significantly increased when compared to Group IPp15 and the control group. Ovarian histopatological assesment scores were significantly higher in Group Pp15 than in Groups Pp10 and IPp15. Conclusions. Pneumoperitoneum causes injuries to abdominal organ such as the ovaries. The ischemic preconditioning method is more effective in reducing oxidative stress due to laparoscopic pneumoperitoneum than lowpressure pneumoperitoneum methods (Adv Clin Exp Med 2014, 23, 6, 885-892).

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Ethanol Induced Oxidative Stress and Membrane Injury in Rat Erythrocytes

Cited by 27 publications

References 20 publications

The effect of ethanol on erythrocyte carbonic anhydrase isoenzymes activity: An in vitro and in vivo study

The effect of ethanol on erythrocyte carbonic anhydrase isoenzymes activity: An in vitro and in vivo study

Effect of alcohol consumption on haemorheology and osmotic fragility of subjects in Nnewi, South-eastern, Nigeria

The Effects of Different Intraabdominal Pressure Protocols in Laparoscopic Procedures on Oxidative Stress Markers and Morphology in Rat Ovaries

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