Estimation of microbiological propagation and antimicrobial traits of the frequently accessible flowers

Abstract

Present study portrayed a complete microbiological profile of commonly available flowers including Rosa kordesii, Gladiolus hybrid, Acmella oleracea, Nyctanthes arbortristis and Pseudomussaenda flava which were randomly collected from Dhaka city, Bangladesh. The microbial contamination was quantified up to 108 cfu/g. Exploration of specific pathogenic bacteria was estimated within the range of 103 to 108 cfu/g of which Pseudomonas spp. was found in G. hybrid, A. oleracea and P. flava (~106 cfu/g), whereas Escherichia coli and Staphylococcal contamination was evident in almost all samples up to 108 cfu/g. The in vitro antimicrobial activities of the flower extracts were notable against most of the test bacteria. The ethanolic extracts of R. kordesii showed anti-bacterial activity against most of the bacteria except E. coli and Salmonella spp. G. hybrid extracts showed activity against Klebsiella spp. and Bacillus spp., A. oleracea against E. coli, Pseudomonas spp., Bacillus spp., Klebsiella spp., Staphylococcus spp. and Salmonella spp., P. flava against Pseudomonas spp. and Bacillus spp., and N. arbortristis against Bacillus spp. The methanol extracts of G. hybrid possessed activity against E. coli, Listeria spp. and Pseudomonas spp., N. arbortristis extracts against E. coli, Vibrio spp., Bacillus spp., Klebsiella spp. and Staphylococcus spp., P. flava extracts against E. coli. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22756 Stamford Journal of Microbiology, Vol.4(1) 2014: 19-23

Artificial Cultivation of Ganoderma lucidum (Reishi Medicinal Mushroom) Using Different Sawdusts as Substrates

“…Due to the increased rate of resistance and several adverse effects for toxicity of the synthetic drugs, natural products like herbs have attracted lots of interest to be a suitable drug alternatives or sources for new drug discoveries in recent years [8,[24][25][26][27][28][29][30][31][32][33][34][35][36][37]. G. lucidum has already proved its competency as medicinally active mushrooms with a wide range of therapeutic and biological effects [3-5, 7, 9, 23].…”

Microbial Contamination and Detection of Antibacterial Activity of Syzygium aromaticum against Food Borne Pathogens

“…for 3 hours. All the inoculated plates were incubated at 37 °C for 24 hours except Sabouraud Dextrose agar plates, which were incubated at 25 °C for 48 hours 17 .…”

“…Crude extraction 3.33 g of dried and blended sample were soaked in 10 mL of normal sterile saline (maintaining the ration of 25 g of sample with 75 mL of normal saline) 17 .…”

“…Antibiotic disc of gentamicin 10 µg (Oxoid Ltd., Basingstoke, Hampshire, England) was used as a positive control. Plates were incubated at 37 °C for 12-18 h, and were examined for the determination of zone of inhibitions (mm) 17,22,23 .…”