Estimation of genetic diversity in a natural population of cambui tree (Myrciaria tenella O. Berg) using ISSR markers

Abstract

Cambui (Myrciaria tenella O. Berg) is a native species from Brazil, which belongs to the family Myrtaceae. Molecular characterization is one of the most used tools for the study of the biotechnological potential of species because the diversity level between individuals can be inferred. Analysis of genetic diversity is fundamental to the direction of the strategies necessary to form and maintain a germplasm. This study aimed to evaluate the genetic diversity in a natural population of cambui using inter-simple sequence repeat (ISSR) molecular markers. The natural population, which provided the plant material, is found at the Private Reserve of Natural Heritage of Caju, which belongs to the experimental field of Embrapa Tabuleiros Costeiros, in the municipality of Itaporanga d'Ajuda, SE, Brazil. Young leaves of each individual were collected for DNA extraction and analysis of PCR-ISSR. Thirty primers were tested and the top 10 were selected. The use of these primers resulted in 71 fragments with 98.3% polymorphism. Similarity of individuals ranged between 0.30 and 0.92. The most similar individuals were C13 and C17 and the most distant were C1 and C41. Through UPGMA, six distinct groups were identified. This information may be used for conservation of these genetic resources, germplasm exchange, creation of germplasm bank and in future studies with this species.

Genetic diversity of cambui trees (Myrciaria floribunda (West ex Willd) O. Berg) differentiated by the color of the fruit

“…This index can vary from 0 to 1, and the diversity is considered lower when values are closer to zero (Silva et al, 2015). In this study, the cambuizeiros with fruits of distinct coloring displayed an intermediate level of genetic diversity, similar to that observed by Santana et al (2016), which obtained (I) of 0.52.…”

“…In natural cambuizeiro population, Santana et al (2016) used 10 primers and obtained 70 polymorphic bands (98.3%). For the characterization of jabuticaba trees (Plinia sp.…”

“…Several native fruit trees species with ample potential of use are found in this biome. In the state of Sergipe, for example, researches have been conducted with jenipapeiro (Genipa americana L.) (Silva et al, 2015;, cambuizeiro (Myrciaria tenella O. Berg) (Santana et al, 2016) and mangabeira (Hancornia speciosa Gomes) .…”

“…Its usage has been established for several applicabilities, both for the conservation biology as well as for the molecular and systematic ecology (Gharbawi, 2015). In cambuizeiro, Santana et al (2016) used 10 ISSR primers to characterize 50 plants of a natural population in Sergipe.…”

Myrciaria dubia, an Amazonian fruit: population structure and its implications for germplasm conservation and genetic improvement

“…Santana et al (2016) used ISSR markers to study the genetic diversity of Myrciaria tenella and found 71 polymorphic bands. Brandão et al (2011) evaluated the genetic structure of Myrcia splendens using 10 ISSR markers and found 70 polymorphic bands.…”