Estimation of catecholamines in urine by high performance liquid chromatography with electrochemical detection

Determination of free and total catecholamines in human urine by HPLC with fluorescence detection

“…The concentrations and the ratios of free to total forms in individual CAs were in agreement with the reported data (Kissinger et a l , 1975;Moyer et al, 1979;Goldstein, 1983). However, the concentrations of the individual free CAs did not correlate well with those of respective total CAs; the correlation coefficients for NE, E and DA were 0.47,0.67 and 0.50, respectively.…”

“…The former do not permit highly sensitive determination of all the CAs, and the latter require complicated clean-up procedures to remove many oxidizable substances (to which the detector responds) that may occur in urine. Total (the sum of free and conjugated) CAs in urine have been assayed by these HPLC methods after perchloric acidhydrolysis of conjugated CAs to the free forms (Kissinger et al, 1975;Ueda et al, 1977;Elchisak and Carlson, 1982).…”

Genetic and Environmental Effects on Urinary Kallikrein, Catecholamines, Sodium, Potassium, Urea Nitrogen and Inorganic Sulfate Sulfur Levels in School-Age Twins

“…Therefore, heredi tary control of urinary epinephrine excretion cannot be invoked to the same extent as for urinary kallikrein excretion. Urinary cate cholamine excretion has been demonstrated to be effected by environmental factors in cluding stress [15] and physical exercise [5], For urinary' levels of sodium and potas sium, intraclass correlation coefficients for monozygotic twins were significant (0.36, p < 0.05 and 0.57, p < 0.001, respectively) and greater than the respective values for dizygot ic twins. Furthermore, the genetic effects on both urinary electrolytes are greater than each corresponding environmental effect.…”

“…Urinary catecholamine was analyzed by high-performance liquid chromatog raphy following the method of Kissinger et al [5], Uri nary urea nitrogen and inorganic sulfate sulfur as indi cators of protein intake were measured following the method of Simmons [6,7], With regard to urine col lection, one monozygotic cotwin and one dizygotic cot win did not participate, thus reducing the number of twin pairs to 35 monozygotic and 19 dizygotic. Uri nary variables were treated in the following three ways.…”

A Simple, Optimised and Rapid Assay for Urinary Free Catecholamines by HPLC with Electrochemical Detection

“…7,10 The present study was undertaken to investigate the above variables with a view to proposing a simple, reliable method for the determination of urinary free catecholamines. The technique of HPLC was chosen because of its sensitivity and specificity, both of which can be further enhanced by the use of electrochemical detection.…”