Determination of free and total catecholamines in human urine by HPLC with fluorescence detection

Nohta, Hitoshi; Mitsui, Akane; Umegae, Yoshihiko; Ohkura, Yosuke

doi:10.1002/bmc.1130020104

Cited by 18 publications

(5 citation statements)

References 20 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Blood samples were drawn from retro‐orbital plexus of sevoflurane‐anaesthetized control and experimental mice immediately, 1, and 2 h after the release from 2 h restraint and collected into heparin‐coated tubes. After centrifugation at 1700 g for 10 min at 4°C, plasma was collected and catecholamines including epinephrine, norepinephrine, and dopamine were measured by column‐switching high‐performance liquid chromatography (HPLC) with fluorometric detection as described previously with slight modifications [35,36]. Briefly, after protein precipitation using 5% perchloric acid, plasma samples were injected into a reversed‐phase gel column (TSK precolumn CA1, TOSOH, Tokyo, Japan) to remove water‐soluble components, and into a cation exchange gel column (TSK precolumn CA2, TOSOH) to remove anionic components.…”

Section: Methodsmentioning

confidence: 99%

Acute stress reduces intraparenchymal lung natural killer cells via beta-adrenergic stimulation

Kanemi

Zhang

Sakamoto

et al. 2004

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryThere are lines of evidence that natural killer (NK) cells are sensitive to physical and psychological stress. Alterations in the immune system including NK cells are known to differ among tissues and organs. The effect of stress on the lung immune system, however, has not been well documented in spite of the fact that the lungs always confront viral or bacterial attacks as well as tumour cell metastasis. In this study, we intended to investigate the effect of restraint stress on lung lymphocytes including NK cells. C57BL/6 mice were exposed to 2 h restraint stress. The concentration of plasma epinephrine significantly rose immediately after the release from restraint as compared to home-cage control mice. Flow cytometric analysis revealed that the numbers of most lymphocyte subsets including NK cells were decreased in the lungs and blood but not in the spleen, immediately after restraint stress. Immunohistochemical examination revealed that the number of NK cells was decreased in the intraparenchymal region of the lungs, while the number of alveolar macrophages did not change. The decrease in the number of NK cells in the lungs and blood was reversed by the administration of propranolol, a nonselective beta adrenergic antagonist. Taken together, our findings suggest that acute stress reduces the number of intraparenchymal lung NK cells via activation of beta adrenergic receptors.

show abstract

Section: Methodsmentioning

confidence: 99%

Acute stress reduces intraparenchymal lung natural killer cells via beta-adrenergic stimulation

Kanemi

Zhang

Sakamoto

et al. 2004

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…These methods, however, are not selective for indoleamines and catecholamines. On the other hand, highly selective LC methods based on specific fluorescence derivatizations have been developed [11][12][13]. Although these approaches are sensitive and specific for their respective target amines, they cannot be applied to the simultaneous determination of a number of bioamines.…”

Section: Introductionmentioning

confidence: 98%

Liquid chromatography method for detecting native fluorescent bioamines in urine using post-column derivatization and intramolecular FRET detection

Yoshitake

Nohta

Ogata

et al. 2007

Journal of Chromatography B

Self Cite

View full text Add to dashboard Cite

“…29 These methods, however, give fluorescence with all amino compunds, so they are not selective for indoleamines and catecholamines. On the other hand, highly selective LC methods based on specific fluorescence derivatizations have been developed using benzylamine 30 for 5-hydroxyindoleamines and trihydroxyindole, 31,32 ethylenediamine, 33 and 1,2-diphenylethylenediamine 34,35 for catecholamines. Although these approaches are sensitive and specific for their respective target amines, they cannot be applied to the simultaneous determination of a number of bioamines.…”

mentioning

confidence: 99%

Selective Determination of Native Fluorescent Bioamines through Precolumn Derivatization and Liquid Chromatography Using Intramolecular Fluorescence Resonance Energy Transfer Detection

et al. 2005

Self Cite

View full text Add to dashboard Cite

In this paper, we introduce a novel approach for the highly selective and sensitive analysis of native fluorescent bioamines (indoleamines and catecholamines). This method is based on intramolecular fluorescence resonance energy transfer (FRET) detection in a liquid chromatography (LC) system following precolumn derivatization of the bioamines' amino groups. In this detection process, we monitored the FRET from the native fluorescent moieties (donor) to the derivatized fluorophore (acceptor). From a screening study involving 15 fluorescent reagents, we found that o-phthalaldehyde (OPA) generated the FRET most effectively. The OPA derivatives of the native fluorescent bioamines emitted OPA fluorescence (445 nm) through an intermolecular FRET process when they were excited at the excitation maximum wavelengths of the native fluorescent bioamines (280 nm). The generation of FRET was confirmed through comparison with the analysis of a nonfluorescent amine (isoleucine) performed using LC and a three-dimensional fluorescence detection system. We were able to separate the OPA derivatives of the indoleamines and catecholamines when performing LC on an ODS column. The detection limits (signal-to-noise ratio, 3) for the indoleamines and catecholamines, at a 20-muL injection volume, were 17-120 and 28-200 fmol, respectively. The sensitivity of the intramolecular FRET-forming derivatization method is higher than those of systems that take advantage of both native fluorescence detection (i.e., without derivatization) and the conventional detection of OPA derivatives. Furthermore, this method provides enough selectivity and sensitivity for the determination of the indoleamines present in the urine of healthy humans.

show abstract

Determination of free and total catecholamines in human urine by HPLC with fluorescence detection

Cited by 18 publications

References 20 publications

Acute stress reduces intraparenchymal lung natural killer cells via beta-adrenergic stimulation

Acute stress reduces intraparenchymal lung natural killer cells via beta-adrenergic stimulation

Liquid chromatography method for detecting native fluorescent bioamines in urine using post-column derivatization and intramolecular FRET detection

Selective Determination of Native Fluorescent Bioamines through Precolumn Derivatization and Liquid Chromatography Using Intramolecular Fluorescence Resonance Energy Transfer Detection

Contact Info

Product

Resources

About