Liquid chromatography method for detecting native fluorescent bioamines in urine using post-column derivatization and intramolecular FRET detection

Yoshitake, Makoto; Nohta, Hitoshi; Ogata, Shigenobu; Todoroki, Kenichiro; Yoshida, Hideyuki; Yoshitake, Takashi; Yamaguchi, Masatoshi

doi:10.1016/j.jchromb.2007.08.028

Cited by 27 publications

(16 citation statements)

References 20 publications

(19 reference statements)

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“…Concentrations of Agm, E and DA in human urine were found to be in the range of 2.61 × 10 −7 to 4.30 × 10 −7 M, 0.81 × 10 −7 to 1.12 × 10 −7 M, and 8.76 × 10 −7 to 11.21 × 10 −7 M, respectively. These results were closely comparable with those obtained by HPLC methods [41, 42] and CE-LIF methods [19]. Interestingly, histamine and tyramine were not detected in the samples analyzed.…”

Section: Resultssupporting

confidence: 90%

Quantification of biogenic amines by microchip electrophoresis with chemiluminescence detection

Zhao

Huang

Shi

et al. 2009

Journal of Chromatography A

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A highly sensitive microchip electrophoresis (MCE) method with chemiluminescence (CL) detection was developed for the determination of biogenic amines including agmatine, epinephrine, dopamine, tyramine, and histamine in human urine samples. To achieve a high assay sensitivity, the targeted analytes were pre-column labeled by a CL tagging reagent, N-(4-aminobutyl)-N-ethylisoluminol (ABEI). ABEI-tagged biogenic amines after MCE separation reacted with hydrogen peroxide in the presence of horseradish peroxidase (HRP), producing CL emission. Since no CL reagent was added to the running buffer, the background of the CL detection was extremely low, resulting in a significant improvement in detection sensitivity. Detection limits (S/N =3) were in the range from 5.9 × 10 −8 to 7.7 × 10 −8 M for the biogenic amines tested, which were at least 10 times lower than those of the MCE-CL methods previously reported. Separation of a urine sample on a 7-cm glass/poly (dimethylsiloxane) (PDMS) microchip channel was completed within 3 min. Analysis of human urine samples found that the levels of Agm, E and DA were in the ranges of 2.61 ×10 −7 to 4.30×10 −7 M, 0.81×10 −7 to 1.12×10 −7 M, and 8.76× −7 to 11.21×10 −7 M (n=4), respectively.

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Section: Resultssupporting

confidence: 90%

Quantification of biogenic amines by microchip electrophoresis with chemiluminescence detection

Zhao

Huang

Shi

et al. 2009

Journal of Chromatography A

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“…Common assays on dopamine quantification in biological fluids include LC-ultraviolet detection (LC-UV), LC-fluorescence detection (LC-FLD), LC-electrochemical detection (LC-ECD) and LC-mass spectrometry (LC-MS) [3][4][5][6]. The major limitations of these assays include insufficient sensitivity, laborious sample preparation and potential interferences from structurally similar endogenous and exogenous compounds [7][8].…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of dopamine in human plasma by a highly sensitive LC–MS/MS assay: Application in preterm neonates

Zhang

Chow

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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“…Low levels of dopamine are related to neurological disorders, such as schizophrenia, epilepsy, Parkinson's disease and HIV infection [16,17]. [18][19][20], spectrophotometry [21][22][23] and electrochemistry [15,16,[24][25][26][27][28][29][30]. Several modified sensors are reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

Bean sprout peroxidase biosensor based on l-cysteine self-assembled monolayer for the determination of dopamine

Moccelini

Fernandes

Vieira

2008

Sensors and Actuators B: Chemical

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Liquid chromatography method for detecting native fluorescent bioamines in urine using post-column derivatization and intramolecular FRET detection

Cited by 27 publications

References 20 publications

Quantification of biogenic amines by microchip electrophoresis with chemiluminescence detection

Quantification of biogenic amines by microchip electrophoresis with chemiluminescence detection

Quantitative determination of dopamine in human plasma by a highly sensitive LC–MS/MS assay: Application in preterm neonates

Bean sprout peroxidase biosensor based on l-cysteine self-assembled monolayer for the determination of dopamine

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