Estimation of acute oral toxicity in rates by determination of the approximate lethal dose rather than the LD₅₀

Abstract

The approximate lethal dose (ALD) to rats following oral administration of nine chemicals was determined. The ALD (lowest dose at which death was produced) for each chemical was used to define the general order of toxicity as being either extremely, highly, moderately, slightly, or practically non-toxic. For each of the nine chemicals tested, the category indicated by the ALD was identical with the category determined in the more extensive LD50 studies. In this study, an average of 6.8 rats was needed to determine the ALD while an average of 56.3 rats was needed to determine the LD50.

Acute Toxicity Evaluation of Ethanol Extract of Indonesian Velvet Beans

“…Accordingly, the determination of levels of ALT and AST is required as advanced research to ensure the effect of the ethanol extract of Mucuna pruriens seeds in the liver. However, in other reports related to Mucuna pruriens extracts showed hepatoprotective activity such as the ethanol-water extract of Mucuna pruriens leaves from Nigeria [8], and the ethanol extract of Mucuna pruriens seeds from India [9,37]. These findings clarify that the content and composition of compounds in plants depending on the part of the plant, a region of origin, and solvents used to extract.…”

“…This is a class with the lowest toxicity level. Based on the study conducted by Kennedy, a substance with an LD50 CONCLUSION value greater than 5000 mg/kg orally is considered safe and practically non-toxic [37].…”

Acute and Subacute Oral Toxicity Evaluation of Crude Antifungal Compounds Produced by Lactobacillus plantarum HD1 in Rats

“…In this study, the LD 50 could not be estimated, and it is likely to be more than 5.0 g/kg. Chemical materials with a LD50 in the range of 5.0 g/kg by oral route were considered to have low-acute toxicity (22). Therefore, it was shown that the acute toxicity of crude antifungal compounds produced by L. plantarum HD1 is practically null via oral route.…”

Anti-inflammatory and hepatoprotective potentials of the aerial parts of <i>Silene villosa</i> Caryophyllaceae methanol extract in rats

“…Generally, the lower the value of the LD 50 , the higher the toxicity of the compound. Thus, the methanolic extract of S. villosa can be classified as non-toxic as compounds having oral LD 50 value of more than 4 g/kg are considered as being nontoxic [16].…”