Estimating the Plasma and Serum Activity Levels of Aspartate Aminotransferase and Alanine Aminotransferase, in Live Animals Using Regression Model

Ahur, Victor Masekaven; Agada, P.O.; Saganuwan, S. A.

doi:10.3923/tasr.2012.748.757

Cited by 4 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liver degeneration cause by these diseases is accompanied by leakage of various enzymes from injured hepatocytes into the blood. Aminotranferase because their activity can be detected in very low amount are most useful in the monitoring of people suffering from these diseases since these enzymes activities are high in liver and thus are likely to be among the proteins leaked from damaged hepatocytes [31].…”

Section: Resultsmentioning

confidence: 99%

The Effect of Benniseed Oil (Sesamum indicum Linn) on Induced Hypercholesterolemia in Albino Rats

Olasunkanmi

Ajayi

James

2019

JABB

View full text Add to dashboard Cite

The study was designed to investigate the antioxidant and antitoxicological potential of Sesamum indicum Linn seed (benni seed) oil on hypercholesterolemic rat. Albino rats weighing between 120-130 g were divided into two groups, group 1, was fed with normal rat diet(normal control), groups 2 was fed 1% cholesterol and 20% soya bean oil for 3 weeks to induce hypercholesterolemic state. Group 2 was later divided into groups 2, 3 and 4, group 2 was untreated, groups 3 and 4 were later fed with 5% and 10% Sesamum indicum L. seed oil incorporated in normal rat diet for another 6 weeks respectively. Significant (P<0.05) increase in lipid peroxidation (TBARs) and reduction in superoxide dismutate (SOD) and catalase (CAT) was observed in the liver of the hypercholesterolemic rats as compared to the normal control. At the same time, the oxidative stress causes significant (P<0.05) increase in serum level of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Alkaline phosphatase (ALP) of hypercholesterolemic rats. Administering Sesamum indicum Linn seed oil significantly reduced (P<0.05), serum ALT, AST, ALP and lipid peroxidation, elevated the level of SOD and CAT in the liver of Sesamum oil treated hypercholesterolemic rats. These findings indicate that Sesamum indicum Linn seed oil show possible prevention of hepatic stress by high cholesterol and free radical mediated oxidative stress in cells of experimental hypercholesterolemic rats.

show abstract

Section: Resultsmentioning

confidence: 99%

The Effect of Benniseed Oil (Sesamum indicum Linn) on Induced Hypercholesterolemia in Albino Rats

Olasunkanmi

Ajayi

James

2019

JABB

View full text Add to dashboard Cite

show abstract

“…Blood was collected after 7 days from all mice groups. The biochemical effects of selected compounds which shows antiporoliferative potency, on some liver enzymes such as aspartate, alanine aminotransferases (AST and ALT) [15] and alkaline phosphatase (ALP) [16], were analyzed using a blood auto analyzer (Olympus AV 400, Tokyo, Japan). Moreover, albumin [17], globulins [18], creatinine [19], total lipids [20], cholesterol [21], triglycerides and bilirubin [22] in serum of mice were evaluated in comparison to 5-FU and DOX.…”

Section: -2 Biochemical Analysismentioning

confidence: 99%

Synthesis and Evaluation of the Antiproliferative Potency and Induced Biochemical Parameters of Novel Pyrazolones Derivatives Towards Hepatocellular Carcinoma

Soliman

Roaiah

2022

Egypt. J. Chem.

View full text Add to dashboard Cite

There is currently a great deal of interest in the chemistry of pyrazol-5-ones and their related compounds due to their wide range of biological and pharmacological activities. A variety of compounds having a pyrazol-5-one moiety as a structural unit have been synthesized and studied with interest centered on their potential pharmaceutical activity. Previous researches revealed that pyrazol-5-one moiety is an important pharmacophore which exhibits outstanding biological activities such as antioxidant, anti-fungal, antibacterial, antidepressant, anticonvulsant, anti-inflammatory, antipyretic and antitumor. The present work focused on studying the synthesis of novel pyrazoline derivatives and screening of some selected their antiproliferative potency of some selected derivatives namely compounds 1, 2, 3, 4, 5, 6, 7, 8 and 9 towards hepatocellular carcinoma cell line (HEPG2). Results indicated that the selected compounds 9, 7 and 6 were the most active derivatives towards HEPG2 cell line with IC50 equals 3.74, 3.92 and 4.31µg/mL respectively. The newly prepared selected derivatives showed interesting antiproliferative potency in comparison to the traditional anticancer drugs: 5 Fluorouracil and doxorubicin. However, the potent compounds needs more pharmacological tests.

show abstract

“…Tests. Serum levels of aspartate transferase (AST) and alanine transferase (ALT) were analyzed according to the method described in [27,28]. Serum levels of urea and creatinine were determined by the Jaffe reaction [29], while total cholesterol and TAGs were determined according to methods described by Carlson and Goldfarb [30].…”

Section: Estimation Of Liver and Kidney Functionmentioning

confidence: 99%

Hypoglycemic and Toxic Effect ofMorus mesozygiaLeaf Extract on the Liver and Kidneys of Alloxan-Induced Hyperglycemic Wistar Rats

Tirwomwe

Echoru

Maseruka

et al. 2019

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Purpose. We investigated the hypoglycemic and toxic effect of Morus mesozygia leaf extract on the liver and kidneys of alloxan-induced hyperglycemic wistar rats. Method. Phytochemical analysis was done. Diabetes was induced by the use of alloxan monohydrate in six groups of rats, i.e., 200 mg/kg, 400 mg/kg, 800 mg/kg, glibenclamide, normal saline, and normal control group. Blood glucose was measured at the time of inoculation, then at 1, 2, 3, and 4 hours after. After 14 days, rats were killed under anesthesia; blood collected for measurement of total protein, albumin, TAGs, cholesterol, AST, ALT, urea, and creatinine; and whole tissue of liver and kidneys used for histological studies. Results. The extract possessed antidiabetic effects between 400 mg/kg and 800 mg/kg doses, which we attributed to the presence of flavonoids, tannins, terpenoids, and amino acids. There was a drop in total protein and albumin with no statistical significance (P≥0.05). The changes in levels of ALT, TAGs, cholesterol, AST, creatinine, and urea were not statistically different from the standard diabetic drug. The extract was protective against histological damage as there were no significant lesions suggestive of toxicities in the liver and kidneys at doses below 800 mg/kg. Conclusion. We established credible evidence that Morus mesozygia leaf extract has hypoglycemic effects between 400 mg/kg and 800 mg/kg and that it is safe on the liver and kidneys of wistar rats at doses less than 800 mg/kg.

show abstract

Estimating the Plasma and Serum Activity Levels of Aspartate Aminotransferase and Alanine Aminotransferase, in Live Animals Using Regression Model

Cited by 4 publications

References 13 publications

The Effect of Benniseed Oil (Sesamum indicum Linn) on Induced Hypercholesterolemia in Albino Rats

The Effect of Benniseed Oil (Sesamum indicum Linn) on Induced Hypercholesterolemia in Albino Rats

Synthesis and Evaluation of the Antiproliferative Potency and Induced Biochemical Parameters of Novel Pyrazolones Derivatives Towards Hepatocellular Carcinoma

Hypoglycemic and Toxic Effect ofMorus mesozygiaLeaf Extract on the Liver and Kidneys of Alloxan-Induced Hyperglycemic Wistar Rats

Contact Info

Product

Resources

About