1998
DOI: 10.1590/s0066-782x1998000200007
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Estereologia do miocárdio de ratos jovens e idosos

Abstract: Purpose - Estereologia do Miocárdio de Ratos Jovens e Idosos Artigo OriginalAs mudanças estruturais do miocárdio decorrente do envelhecimento ainda não estão totalmente esclarecidas na literatura médica. Sabe-se que, com o avançar da idade, há diminuição do número e hipertrofia dos miócitos cardíacos. A estereologia é um método de morfologia quantitativa que permite determinar com precisão estas alterações do miocárdio na idade avançada 1 .

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Cited by 15 publications
(7 citation statements)
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References 13 publications
(17 reference statements)
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“…24 The cellular diameter increases from 5 µm in the newborn to 16 µm in the adult rat. 25 According to our findings, therefore, the myocytes from fetal rats carried by diabetic mothers have the size of myocytes from the adult rat heart. Gestational diabetes in our rats was associated with a significant fetal hyperglycemia.…”
Section: Discussionmentioning
confidence: 51%
“…24 The cellular diameter increases from 5 µm in the newborn to 16 µm in the adult rat. 25 According to our findings, therefore, the myocytes from fetal rats carried by diabetic mothers have the size of myocytes from the adult rat heart. Gestational diabetes in our rats was associated with a significant fetal hyperglycemia.…”
Section: Discussionmentioning
confidence: 51%
“…The procedure may be repeated several times if we want more security in cases in which the material is highly anisotropic [16]. Then fragments of myocardium were fixed in 10% buffered formalin and dehydrated in a increasing series of alcohols, they were cleared in xylene, embedded in paraffin, sectioned in 7 lm thick cuts and stained with picrosirius red for light microscopy analysis [17].…”
Section: Methodsmentioning
confidence: 99%
“…The samples were obtained using the methods described by Águila, Apfel, and Mandarim-De-Lacerda, (1997). Thirty minutes prior to euthanasia, an intraperitoneal injection of heparin (25,000 IU) was administered and the animals were anesthetized with an intraperitoneal injection of a combination of ketamine and xylazine (40 mg/kg of body weight), as proposed by Seraphim, Nunes, and Machado, (2001).…”
Section: Euthanasiamentioning
confidence: 99%