Fetal myocardial hypertrophy in an experimental model of gestational diabetes

Menezes, Honório Sampaio; Barra, Marinez Bizarro; Belló, André R.; Martins, Carlos Otávio Damas; Zielinsky, Paulo

doi:10.1017/s1047951101000956

Cited by 23 publications

(17 citation statements)

References 15 publications

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“…We tested the hypothesis that the fetal LASF would be decreased in fetuses of diabetic mothers compared to fetuses of normal control mothers. The results obtained so far are compatible with this assumption, probably as a result of increased myocardial mass and left ventricular hypertrophy in fetuses of diabetic mothers (30).…”

Section: Resultssupporting

confidence: 83%

Alternative parameters for echocardiographic assessment of fetal diastolic function

Zielinsky

Nicoloso

Firpo

et al. 2004

Braz J Med Biol Res

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Alternative methods to assess ventricular diastolic function in the fetus are proposed. Fetal myocardial hypertrophy in maternal diabetes was used as a model of decreased left ventricular compliance (LVC), and fetal respiratory movements as a model of increased LVC. Comparison of three groups of fetuses showed that, in 10 fetuses of diabetic mothers (FDM) with septal hypertrophy (SH), the mean excursion index of the septum primum (EISP) (ratio between the linear excursion of the flap valve and the left atrial diameter) was 0.36 ± 0.09, in 8 FDM without SH it was 0.51 ± 0.09 (P = 0.001), and in the 8 normal control fetuses (NCF) it was 0.49 ± 0.12 (P = 0.003). In another study, 28 fetuses in apnea had a mean EISP of 0.39 ± 0.05 which increased to 0.57 ± 0.07 during respiration (P < 0.001). These two studies showed that the mobility of the septum primum was reduced when LVC was decreased and was increased when LVC was enhanced. Mean pulmonary vein pulsatility was higher in 14 FDM (1.83 ± 1.21) than in 26 NCF (1.02 ± 0.31; P = 0.02). In the same fetuses, mean left atrial shortening was decreased (0.40 ± 0.11) in relation to NCF (0.51 ± 0.09; P = 0.011). These results suggest that FDM may have a higher preload than normal controls, probably as a result of increased myocardial mass and LV hypertrophy. Prenatal assessment of LV diastolic function by fetal echocardiography should include analysis of septum primum mobility, pulmonary vein pulsatility, and left atrial shortening.

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Section: Resultssupporting

confidence: 83%

Alternative parameters for echocardiographic assessment of fetal diastolic function

Zielinsky

Nicoloso

Firpo

et al. 2004

Braz J Med Biol Res

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“…The fetal heart is one of the organs most affected by diabetes, which complicates pregnancy and poses a risk of cardiac malformations in up to 8.5% of live-born IDM 24 . In this study, ODR at birth had greater heart length, heart weight and heart-to-body weight ratios than animals in the control group, which confirms the findings by Menezes 19 . On the 11th day of life, all measures were statically equal, which suggests the regression of cardiomegaly in the ODR.…”

Section: Discussionsupporting

confidence: 91%

Regression of gestational diabetes induced cardiomegaly in offspring of diabetic rat

et al. 2009

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Purpose:To compare body weight and length, heart weight and length, heart-to-body weight ratio, glycemia, and morphometric cellular data of offspring of diabetic rats (ODR) and of normal rats (control). Methods: Diabetes was induced in 3 pregnant Wistar rats, bearing 30 rats, on the 11th day after conception by intraperitoneal injection of 50 mg/kg of streptozotocin. Six normal pregnant Wistar rats, bearing 50 rats, made up the control group. Morphometric data were obtained using a scale for the weight, length, heart and body measurements. Morphometric cellular data were obtained by a computer assisted method applied to the measurements of myocytes. Statistical analysis utilized Student's t-test, ANOVA and Levene test. Results: Control offspring had greater mean body weight and length than offspring of diabetic rats (p < 0.001). Heart weight and length and heart-to-body ratios of newborn rats differed between groups at birth (p < 0.001), but showed no difference at 21 days. Mean nuclei area and perimetric value of the myocytes decrees throughout the first 21 days of life (p < 0.01) in the diabetic group. Conclusions: Heart hypertrophy on the offspring of diabetic rats at birth was demonstrated by the significant difference between the groups. After the eleventh day, no difference was found, which confirmed regression of cardiomegaly. The significant difference between the first and the 21th day of life, for nuclei area feature, demonstrate regression of cardiac hypertrophy in the offspring of diabetic rats. Key words: Diabetes, Gestational. Cardiomyopathy, Hypertrophic. Rats. RESUMO Objetivo:Comparar as medidas cardíacas e a morfometria celular miocárdica dos filhotes de ratas diabéticas (FRD) com filhotes de ratas normais (FRN). Métodos: Foram estudados 30 filhotes de 3 ratas Wistar com diabetes gestacional induzido por 50mg/kg de estreptozotocina, no 11° dia após a concepção. O grupo controle foi de 50 filhotes de 6 ratas Wistar normais. As medidas de comprimento, peso corporal e peso cardíaco foram realizadas com paquímetro e balança e as medidas celulares por analisador computadorizado de imagem. A análise estatística usou o Teste t de Student, ANOVA e teste de Levene. Resultados: A média de peso e comprimento dos filhotes, desde o nascimento até os 21 dias de vida, foi significativamente maior (p<0,001) no grupo dos FRN. O peso, tamanho cardíaco e a proporção cardíaca dos FRD, ao nascimento, foi, significativamente, maior (p<0,001), regredindo ao longo dos 21 dias de vida. Os FRD apresentaram uma regressão significativa da área e perímetro nuclear (p<0,01) do nascimento aos 21 dias de vida, o mesmo não ocorrendo no grupo controle. Conclusões: Os FRD apresentaram, ao nascimento, maior tamanho cardíaco, maior peso cardíaco e maior proporção peso cardíaco-peso corporal do que FRN, havendo igualdade estatística entre os dois grupos a partir do 11° dia de vida. Houve diferença significativa entre o nascimento e o 21 o dia de vida nas medidas celulares demonstrando regressão da hipertrofia miocárdica nos...

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“…11 To understand cardiac hypertrophy in newborns, rat models have been established related both to diabetic pregnancies and induction with corticosteroids. [12][13][14] These models mimic the human neonatal phenotype. The rats from streptozotocin-induced diabetic mothers had significant asymmetrical septal hypertrophy, and underwent a spontaneous regression within the first 4 weeks of life.…”

mentioning

confidence: 99%

Molecular characterisation of neonatal cardiac hypertrophy and its regression

Gopinath

Trent

2004

Cardiol Young

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Neonatal cardiac hypertrophy associated with diabetic pregnancy is transient and regresses naturally, but is associated with increased morbidity and mortality. This study was undertaken to analyse the changes in expression of 5 cardiac genes, including atrial natriuretic peptide, alpha- and beta-myosin heavy chain, and cardiac and skeletal alpha-actin genes, using a rat neonatal model, in which cardiac hypertrophy was induced via maternal diabetes. In the hypertrophied left ventricle of neonates from diabetic mothers, the levels of mRNA from all the above genes except skeletal alpha-actin were increased by between 1.8- and 12-fold compared with the controls at birth (p < 0.05). In the first 28 days, the level of mRNA for alpha-myosin heavy chain increased slightly, while that for atrial natriuretic peptide and beta-myosin heavy chain decreased continuously similar to the controls, but at a significantly faster rate. No significant difference between the two groups of neonates was observed in all 5 genes after 1 month, indicating complete regression. Expression of 5 cardiac genes in the neonatal cardiac hypertrophy was characterised in both hypertrophic and regressive phases. Hypertrophic regression provides a unique model for the testing of new drugs or genetic modifying factors in cardiac hypertrophy.

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Fetal myocardial hypertrophy in an experimental model of gestational diabetes

Abstract: The presence of cellular and morphologic cardiac hypertrophy in fetuses of diabetic rats was demonstrated by the significant difference between the two groups for each analyzed feature.

Cited by 23 publications

References 15 publications

Alternative parameters for echocardiographic assessment of fetal diastolic function

Alternative parameters for echocardiographic assessment of fetal diastolic function

Regression of gestational diabetes induced cardiomegaly in offspring of diabetic rat

Molecular characterisation of neonatal cardiac hypertrophy and its regression

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