Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle

Liszewska, Ewa; Majchrowicz, Lena; Krogulec, Ewelina; Kotulska, Katarzyna; Kaczmarek, Leszek; Kalita, Katarzyna; Dobrzyń, Agnieszka; Jaworski, Jacek

doi:10.1016/j.scr.2021.102225

Cited by 7 publications

(5 citation statements)

References 3 publications

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“…To obtain iAstrocytes, we opted for the monolayer-based approach as it allows to generate a substantial number of cells amenable to extensive functional tests. We took advantage of the wellestablished procedure to obtain cortical neural progenitors (iNP) and subsequently astrocytes (iAstrocytes) 20 from a panel of primate iPS lines 21 (Fig. 1a, Ext.…”

Section: Resultsmentioning

confidence: 99%

“…2 e,h). Previously published dataset corresponding to bulk RNA-seq profiling of human, chimpanzee, gorilla, and macaque post-mortem cortical tissues 21 was considered in this analysis.…”

Section: Extended Figurementioning

confidence: 99%

“…Distribution of fold changes of expression for EAGs related to extracellular exosome and ID (loci identified in Fig.2 e,h). Previously published dataset corresponding to bulk RNA-seq profiling of human, chimpanzee, gorilla, and macaque post-mortem cortical tissues21 was considered in this analysis. Median LFC of expression of disease-related genes in adult human versus macaque brain cortex.…”

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confidence: 99%

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Molecular signature of primate astrocytes reveals pathways and regulatory changes contributing to the human brain evolution

Ciuba,

Piotrowska,

Chaudhury

et al. 2023

Preprint

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Astrocytes contribute to the development and regulation of the higher-level functions of the brain, the critical targets of evolution. However, the molecular signature of foetal astrocyte evolution in primates is unknown. Here, to address this question, we use human, chimpanzee, and macaque induced pluripotent stem cell-derived foetal astrocytes (iAstrocytes). Human iAstrocytes are bigger and more complex than the non-human primate iAstrocytes. We find loci related to the regulation of cell size with increased expression in the human lineage. Likewise, we uncover that genes and mechanisms implicated in long-range intercellular signalling are activated in the human iAstrocytes. Strikingly, loci downregulated in the human lineage frequently relate to intellectual disability raising new questions on the trade-offs associated with the evolution of the human mind. Using our system, through a multilevel regulome analysis and machine learning, we uncover that functional activation of enhancers coincides with a previously unappreciated, pervasive gain of binding sites of ‘stripe’ transcription factors. In summary, we shed new light on a mechanism driving the acquisition of the regulatory potential of enhancers.

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Section: Resultsmentioning

confidence: 99%

“…2 e,h). Previously published dataset corresponding to bulk RNA-seq profiling of human, chimpanzee, gorilla, and macaque post-mortem cortical tissues 21 was considered in this analysis.…”

Section: Extended Figurementioning

confidence: 99%

mentioning

confidence: 99%

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Molecular signature of primate astrocytes reveals pathways and regulatory changes contributing to the human brain evolution

Ciuba,

Piotrowska,

Chaudhury

et al. 2023

Preprint

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“…The dermal fibroblasts were isolated from a skin biopsy of a 10-year-old healthy girl, Caucasian origin. The protocol for obtaining hiPSCs and their characteristics are presented in the article by Liszewska et al 58 . hiPSCs were grown in Essential 8™ Medium (E8, Thermo Fisher Scientific) supplemented with 5 μM ROCK inhibitor (Tocris) by first 24 h culture, on Matrigel (Corning) coated 6-well plates (Sarsedt).…”

Section: Methodsmentioning

confidence: 99%

Maturation of human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) on polycaprolactone and polyurethane nanofibrous mats

Iwoń,

Krogulec,

Tarnowska

et al. 2024

Sci Rep

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Investigating the potential of human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) in in vitro heart models is essential to develop cardiac regenerative medicine. iPSC-CMs are immature with a fetal-like phenotype relative to cardiomyocytes in vivo. Literature indicates methods for enhancing the structural maturity of iPSC-CMs. Among these strategies, nanofibrous scaffolds offer more accurate mimicry of the functioning of cardiac tissue structures in the human body. However, further research is needed on the use of nanofibrous mats to understand their effects on iPSC-CMs. Our research aimed to evaluate the suitability of poly(ε-caprolactone) (PCL) and polyurethane (PU) nanofibrous mats with different elasticities as materials for the maturation of iPSC-CMs. Analysis of cell morphology and orientation and the expression levels of selected genes and proteins were performed to determine the effect of the type of nanofibrous mats on the maturation of iPSC-CMs after long-term (10-day) culture. Understanding the impact of 3D structural properties in in vitro cardiac models on induced pluripotent stem cell-derived cardiomyocyte maturation is crucial for advancing cardiac tissue engineering and regenerative medicine because it can help optimize conditions for obtaining more mature and functional human cardiomyocytes.

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“…Following this, the iPSCs were differentiated to neural stem cells (NSC) applying the embryoid body-based protocol. iPSC colonies were detached using collagenase (Sigma-Aldrich, St Louis, MO, USA), washed with phosphate-buffered saline (PBS) and transferred to a low attachment culture dish (Liszewska et al 2021).…”

Section: Cell Culturementioning

confidence: 99%

MicroRNA expression profile in TSC cell lines and the impact of mTOR inhibitor

Pawlik

Grabia

Smyczyńska

et al. 2022

Preprint

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The aim of this study was to assess the potential implication of microRNA on tuberous sclerosis (TSC) pathogenesis by performing microRNA profiling on cell lines silencing TSC1 or TSC2 genes using qPCR panels, before and after incubation with rapamycin. (Qiagen). Significant differences in expression were observed between samples before and after rapamycin treatment in 19 miRNAs in TSC1, five miRNAs in TSC2 and seven miRNAs in controls. Of six miRNAs dysregulated before rapamycin treatment in the TSC1 group, three normalized after treatment (miR-21-3p, miR-433-3p, let-7g-3p). Of three miRNAs dysregulated before rapamycin incubation in TSC2 group, one normalized after treatment (miR-1224-3p). Of the miRNAs dysregulated before rapamycin treatment in TSC1 and TSC2 group, two did not normalize after treatment (miR-33a-3p, miR-29a-3p). The results of the possible targets indicated that there are four common genes with seed regions susceptible to the regulation by those miRNAs: ZBTB20, PHACTR2, PLXNC1 and ATP1B4. Our data show no changes in mRNA expression of these targets before rapamycin treatment. These data suggest that miRNA may play a pivotal role in TSC pathogenesis. Some may serve as biomarkers of treatment efficacy. The variability of miR-29a-3p and miR-33a-3p after rapamycin treatment and the lack of changes in their downstream regulatory targets suggests that they might be mTOR independent.

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Establishment of two hiPSC lines (IIMCBi001-A and IIMCBi002-A) from dermal fibroblasts of healthy donors and characterization of their cell cycle

Cited by 7 publications

References 3 publications

Molecular signature of primate astrocytes reveals pathways and regulatory changes contributing to the human brain evolution

Molecular signature of primate astrocytes reveals pathways and regulatory changes contributing to the human brain evolution

Maturation of human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) on polycaprolactone and polyurethane nanofibrous mats

MicroRNA expression profile in TSC cell lines and the impact of mTOR inhibitor

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