Establishment of genetic fidelity of In vitro-raised Lilium bulblets through rapd markers

Varshney, Anushri; Lakshmikumaran, Malathi; Srivastava, Prem S.; Dhawan, Vibha

doi:10.1007/s11627-001-0040-z

Cited by 47 publications

(23 citation statements)

References 28 publications

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“…The general belief is that multiplication involving a callus phase is more vulnerable for DNA damage during micropropagation [24,25]. For an example; in oil palm (Elaeis guineensis Jacq), 5% of somatic embryo-derived palms showed an abnormality that is called the 'mantled' phenotype [26].…”

Section: Resultsmentioning

confidence: 99%

Genetic Fidelity Testing Using SSR Marker Assay Confirms Trueness to Type of Micropropagated Coconut (Cocos nucifera L.) Plantlets Derived from Unfertilized Ovaries

Bandupriya¹,

Iroshini²,

Perera³

et al. 2017

TOPSJ

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Section: Resultsmentioning

confidence: 99%

Genetic Fidelity Testing Using SSR Marker Assay Confirms Trueness to Type of Micropropagated Coconut (Cocos nucifera L.) Plantlets Derived from Unfertilized Ovaries

Bandupriya¹,

Iroshini²,

Perera³

et al. 2017

TOPSJ

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“…No polymorphism was detected during the RAPD and ISSR analysis of in vitro-raised clones. The absence of genetic variation using RAPD and ISSR marker has been reported in micropropagated shoots of Pinus thunbergii (Goto et al 1998) and swertia (Joshi and Dhawan 2007), in vitro-regenerated turmeric (Salvi et al 2001), and in vitro-raised bulblets of Lilium (Varshney et al 2001). …”

Section: Discussionmentioning

confidence: 99%

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Soni

Kaur

2013

Physiol Mol Biol Plants

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A protocol for in vitro propagation was developed for Viola pilosa, a plant of immense medicinal value. To start with in vitro propagation, the sterilized explants (buds) were cultured on MS basal medium supplemented with various concentrations of growth regulators. One of the medium compositions MS basal+0.5 mg/l BA+0.5 mg/l TDZ+0.5 mg/l GA 3 gave best results for in vitro shoot bud establishment. Although the problem of shoot vitrification occurred on this medium but this was overcome by transferring the vitrified shoots on MS medium supplemented with 1 mg/l BA and 0.25 mg/l Kn. The same medium was found to be the best medium for further in vitro shoot multiplication. 100 % root induction from in vitro grown shoots was obtained on half strength MS medium supplemented with 1 mg/l IBA. In vitro formed plantlets were hardened and transferred to soil with 83 % survival. Additionally, conservation of in vitro multiplying shoots was also attempted using two different approaches namely slowing down the growth at low temperature and cryopreservation following vitrification. At low temperature retrieval rate was better at 10°C than at 4°C after conservation of in vitro multiplying shoots. In cryopreservation-vitrification studies, the vitrified shoot buds gave maximum retrieval of 41.66 % when they were precooled at 4°C, while only 16.66 % vitrified shoots were retrieved from those precooled at 10°C. Genetic stability of the in vitro grown plants was analysed by RAPD and ISSR markers which indicated no somaclonal variation among in vitro grown plants demonstrating the feasibility of using the protocol without any adverse genetical effects.

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“…Molecular marker analysis of micropropagated plants has been reported in several plant species (Bhatia et al 2011;Goto et al 1998;Salvi et al 2001;Varshney et al 2001). Martins et al (2004) reported the genetic stability of micropropagated almond plants.…”

Section: Discussionmentioning

confidence: 99%

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Khateeb

Bahar

Lahham

et al. 2012

Physiol Mol Biol Plants

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Moringa peregrina is an endangered species of Moringaceae. M. peregrina is a multipurpose tree with a wide variety of potential uses including its medicinal activity. In our study, a rapid and efficient micropropagation protocol for M. peregrina has been established. In vitro germinated seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different levels of either 6-benzyladenine (BA) or kinetin (Kin). The maximum shoot proliferation of 6.5 shoots per explant with 100 % shoot proliferation rate was observed on MS medium supplemented with 1.0 mg/l BA. On the other hand, MS medium supplemented with 1 mg/l indole-3-butyric acid (IBA) resulted in the maximum number of roots. Micropropagated plants were successfully acclimatized. Genetic stability of micropropagated plants was assessed using Inter-Simple Sequence Repeat (ISSR). The amplification products were monomorphic in all in vitro grown plants. No polymorphism was detected indicating the genetic integrity of in vitro propagated plants. This micropropagation protocol could be useful for raising genetically uniform plants for plant propagation and commercial cultivation.

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Establishment of genetic fidelity of In vitro-raised Lilium bulblets through rapd markers

Cited by 47 publications

References 28 publications

Genetic Fidelity Testing Using SSR Marker Assay Confirms Trueness to Type of Micropropagated Coconut (Cocos nucifera L.) Plantlets Derived from Unfertilized Ovaries

Genetic Fidelity Testing Using SSR Marker Assay Confirms Trueness to Type of Micropropagated Coconut (Cocos nucifera L.) Plantlets Derived from Unfertilized Ovaries

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

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