Genetic Fidelity Testing Using SSR Marker Assay Confirms Trueness to Type of Micropropagated Coconut (Cocos nucifera L.) Plantlets Derived from Unfertilized Ovaries

Abstract

Abstract:Background:

ANALYSIS OF GENETIC STABILITY OF MICROPROPAGATED SUGARCANE IN DIFFERENT SUBCULTURE FREQUENCIES USING SSR MARKER / Analisis Kestabilan Genetik Tebu Hasil Mikropropagasi pada Beberapa Frekuensi Subkultur Menggunakan Marka SSR

“…The in vitro propagation technique of direct organogenesis is the most popular method of reproduction because it can produce large amounts of seedlings that are identical to the mother plant. The results of the study were in line with the previous reports that the in vitro propagation sugarcane varieties GT54-C9 (Alla et al 2017), Albizia procera (Mohammad et al 2016), Gloriosa superba L. (Yadav et al 2013), Mulberry (Saha et al 2016), and Coconut (Bandupriya et al 2017).…”

Recovery, histological observations and genetic integrity in coconut (Cocos nucifera L.) embryogenic calli cryopreserved using encapsulation-dehydration procedure

“…Ovary derived calli were raised using the method described by Bandupriya et al (2017) with the modifi cation that, instead of CRI 72 medium, modifi ed Eeuwens Y 3 medium (Eeuwens, 1976) was used. Welldeveloped embryogenic calli (Figure 1a) from the third or fourth cycle of sub-culturing were used for cryopreservation studies.…”

“…Each alginate bead was cultured for regeneration in a small glass vial containing 15 mL somatic embryo induction medium [modifi ed Eeuwens Y 3 medium containing 95.0 μM 2,4-D and 2.7 gL -1 Glutamine (3G)] for 4 wk. Plant regeneration protocol described by Bandupriya et al (2017) was followed for the regeneration of plantlets.…”

“…SSR primers (Table 1) were selected according to Rivera et al (1999). PCR reaction mixtures were prepared as described by Bandupriya et al (2017). Amplifi cations were performed in thermal cycler (BIO-RAD; MyCyclerTM) programmed at 94 °C for 5 min for initial denaturation, followed by 94 °C for 30 s, annealing temperature depending on the primer used (Table 1) for 30 s and 72 °C for 1 min for 35 cycles followed by a fi nal step of extension at 72 °C for 5 min.…”

“…True-to-type conformity of tissue cultured coconut plants derived from unfertilised ovaries was tested in a previous study with respect to the mother palm and genetic stability at SSR marker loci was confi rmed (Bandupriya et al, 2017). Same SSR marker loci were evaluated for the genetic uniformity of the cryopreserved and non-cryopreserved EC and compared with the untreated controls and the mother palm.…”