Recovery, histological observations and genetic integrity in coconut (Cocos nucifera L.) embryogenic calli cryopreserved using encapsulation-dehydration procedure

Welewanni, I; Perera, Chandrika; Jayasekera, Anil; Bandupriya, D

doi:10.4038/jnsfsr.v48i2.9538

Cited by 4 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Information about cryopreservation of coconut and peach palm is scarce. Welewanni et al. (2020) successfully cryopreserved coconut embryogenic callus for the first time by encapsulation-dehydration in silica gel.…”

Section: Cryopreservation Of Embryogenic Materials In Woody Speciesmentioning

confidence: 99%

“…Information about cryopreservation of coconut and peach palm is scarce. Welewanni et al (2020) successfully cryopreserved coconut embryogenic callus for the first time by encapsulation-dehydration in silica gel. In peach palm, the highest recovery rates (52%) have been achieved by PVS3 and droplet-vitrification (Heringer et al, 2013).…”

Section: Cryopreservation In Palm Tree Speciesmentioning

confidence: 99%

See 1 more Smart Citation

Current status of the cryopreservation of embryogenic material of woody species

Ballesteros,

Martínez,

Sánchez-Romero

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Cryopreservation, or the storage at liquid nitrogen temperatures (-196°C), of embryogenic cells or somatic embryos allows their long-term conservation without loss of their embryogenic capacity. During the last decade, protocols for cryopreservation of embryogenic material of woody species have been increasing in number and importance. However, despite the large experimental evidence proved in thousands of embryogenic lines, the application for the large-scale conservation of embryogenic material in cryobanks is still limited. Cryopreservation facilitates the management of embryogenic lines, reducing costs and time spent on their maintenance, thus limiting the risk of the appearance of somaclonal variation or contamination. Somatic embryogenesis in combination with cryopreservation is especially useful to preserve the juvenility of lines while the corresponding clones are being field-tested. Hence, when tree performance has been evaluated, selected varieties can be propagated from the cryostock. The traditional method of slow cooling or techniques based on vitrification are mostly applied procedures. For example, slow cooling methods are widely applied to conserve embryogenic lines of conifers. Desiccation based procedures, although simpler, have been applied in a smaller number of species. Genetic stability of the cryopreserved material is supported by multiloci PCR-derived markers in most of the assayed species, whereas DNA methylation status assays showed that cryopreservation might induce some changes that were also observed after prolonged subculture of the embryogenic lines. This article reviews the cryopreservation of embryogenic cultures in conifers, fruit species, deciduous forest species and palms, including a description of the different cryopreservation procedures and the analysis of their genetic stability after storage in liquid nitrogen.

show abstract

Section: Cryopreservation Of Embryogenic Materials In Woody Speciesmentioning

confidence: 99%

Section: Cryopreservation In Palm Tree Speciesmentioning

confidence: 99%

Current status of the cryopreservation of embryogenic material of woody species

Ballesteros,

Martínez,

Sánchez-Romero

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

Germplasm Resources: Diversity and Conservation

2021

View full text Add to dashboard Cite

A practical framework for the cryopreservation of palm species

Nguyen

et al. 2023

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Palms (Arecaceae) contribute remarkable economic and environmental values to human life. However, many elite and commercial palm species are becoming critically endangered and demand immediate action to preserve their genetic resources. Cryopreservation has established itself as the definitive in vitro conservation method for recalcitrant-seeded species, such as those within the Arecaceae. Progress in this area has moved steadily forward over the last three decades with the development of various techniques for different explants and palm genotypes and for molecular testing methods to ensure genetic fidelity is maintained in the regenerants. There remains a key challenge to properly identify the components that will enable the long-term conservation of palms using cryopreservation. This review methodically analyzes the state-of-the-art cryopreservation techniques developed for palms and places them within a practical framework. This framework encompasses four underlying components, namely the tissue culture approaches required, the recalcitrant nature of the palm seed, the cryobiology and cryogenic techniques required, and fidelity assessment after cryopreservation. Through a critical analysis of this framework, further optimization of palm cryopreservation protocols and more fundamental studies on the physiological and molecular changes in cryopreserved palm tissues are recommended. The present review helps to showcase a multi-decade global attempt to preserve these mostly recalcitrant species through ex situ collections. From a conservationist’s perspective, this review hopes to stimulate awareness for further concerted efforts in the conservation of rare and endangered plant families. Meanwhile, from a managerial perspective, this work serves to inform decision-makers of the global research effort underway to improve key components of the cryopreservation program for palm species and to encourage funding bodies to appropriately allocate resources to these much-needed research areas.

show abstract

Recovery, histological observations and genetic integrity in coconut (Cocos nucifera L.) embryogenic calli cryopreserved using encapsulation-dehydration procedure

Cited by 4 publications

References 20 publications

Current status of the cryopreservation of embryogenic material of woody species

Current status of the cryopreservation of embryogenic material of woody species

Germplasm Resources: Diversity and Conservation

A practical framework for the cryopreservation of palm species

Contact Info

Product

Resources

About