Establishment of callus from Pyrostegia venusta (Ker Gawl.) Miers and effect of abiotic stress on flavonoids and sterols accumulation

Loredo‐Carrillo, Silvia E.; Santos-Díaz, Ma. de Lourdes; Leyva, Elisa; Santos-Díaz, María del Socorro

doi:10.1007/s13562-012-0161-y

Cited by 14 publications

(16 citation statements)

References 39 publications

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“…However, data indicated that PVP enhanced PPO and POX activity and was non-efficient in protecting R. vesicarius callus from browning. Our result is similar to that reported by Loredo-Carrillo et al (2013) who found that the effect of PVP alone on reducing callus browning was less than in a mixture with citric acid or ascorbic acid.…”

Section: Effect Of Antioxidants On Callus Biomass Browning Intensitysupporting

confidence: 92%

“…This may imply that both kinds of auxins were less favorable for callus productivity of the studied plant. Auxins are fundamental for the establishment of in vitro cultures (Catapan et al, 2002 andPasqua et al, 2003), but concentration and combination of these plant growth regulators must be defined for each species (Loredo-Carrillo et al, 2013). Previous literatures showed that during callogenesis process there is variation in response of plant species and their affinity towards certain type of auxins or cytokinins to callus formation.…”

Section: Effect Of Growth Regulators On Callus Induction Biomass Andmentioning

confidence: 99%

“…Special interests toward callus flavonoids were reported by Wang et al (2013) and Loredo-Carrillo et al (2013). Establishment and optimization of the conditions of callus culture are considered as the initial and essential steps of in vitro production of secondary metabolites.…”

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confidence: 99%

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Effect of Growth Regulators, Carbohydrates and Antioxidant Compounds on Biomass, Flavonoid Accumulation and Enzyme Activity in Callus Cultures of Rumex vesicarius L.

2016

Egypt. J. Bot.

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N THE PRESENT study, growth regulators, sugars and .....antioxidants were investigated for their effect on callus growth, flavonoid content as well as the activity of some related enzymes in callus culture of Rumex vesicarius. The effect of Murashige and skoog media (MS) containing indole butyric acid (IBA), naphthalene acetic acid (NAA), or 2,4-dichlorophenoxy acetic acid (2,4-D) on callus induction from cotyledonary leaf and hypocotyl explants was investigated. Results revealed that the highest callus biomass (fresh and dry weights) was obtained at 0.5 mg l -1 IBA using cotyledonary leaf explant. Both sucrose and fructose promoted a good callus dry mass, while the highest flavonoid content was enhanced with the medium supplemented with fructose. Citric acid was the best antioxidant in reducing the callus browning intensity and significantly inhibiting polyphenol oxidase (PPO) activity by 13.7%. Citric acid also increased the callus dry weight by 26% and total flavonoid content by 67% over control. In conclusion, in vitro culturing of medicinal plant, Rumex vesicarius, enables increase of the biomass production and the yield of its biologically active constituents.Keywords: Rumex vesicarius, Callogenesis, Sugars, Browning, Flavonoid.Plant tissue culture provides a new aspect of nontraditional agriculture of medicinal plants. Recent research and technologies showed the possibility of utilizing tissue culture techniques in large scale production of naturally produced secondary metabolites. Rumex vesicarius L. is a wild winter medicinal plant. It has a wide range of biological and medicinal activities. Phytochemical studies revealed the occurrence of some important flavonoids, vitexin and orientin which

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Section: Effect Of Antioxidants On Callus Biomass Browning Intensitysupporting

confidence: 92%

Section: Effect Of Growth Regulators On Callus Induction Biomass Andmentioning

confidence: 99%

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Effect of Growth Regulators, Carbohydrates and Antioxidant Compounds on Biomass, Flavonoid Accumulation and Enzyme Activity in Callus Cultures of Rumex vesicarius L.

2016

Egypt. J. Bot.

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“…Furthermore, the chemical synthesis of several herbal substances is often impractical due to their structural complexity (OKSMAN-CALDENTEY;INZÉ, 2004). Different bioactive compounds have been found in in vitro culture, especially callus culture such as flavonoids and stilbenes (MANEECHAI et al, 2012), sterols (LOREDO-CARRILLO et al, 2013), cardenolides (SAHIN; VERMA; GUREL, 2013) and phenolic acids (SZOPA; EKIERT, 2014). In vitro plant cell culture has advantages for providing bioactive compounds with a defined production system and short culture periods, which ensures a continuous supply of metabolites of interest; also, cells are free of diseases and are not exposed to seasonal variations.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro plant cell culture has advantages for providing bioactive compounds with a defined production system and short culture periods, which ensures a continuous supply of metabolites of interest; also, cells are free of diseases and are not exposed to seasonal variations. Furthermore, it allows the production of new compounds not normally found in the mother plant (LOREDO-CARRILLO et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Callus induction and bioactive phenolic compounds production fromByrsonima verbascifolia(L.) DC. (Malpighiaceae)

Castro

Braga

Sousa

et al. 2016

Revista Ciência Agronômica

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-This study developed a methodology for callus induction in leaf segments of B. verbascifolia and evaluated the bioactive phenolic compounds production. Leaf explants were cultured in MS medium with 30 g L -1 sucrose, solidified with 7 g L -1 agar supplemented with 2,4-D (0; 4.52; 9.05; 18.10 M) and BAP (0; 4.44; 8.88; 17.75 M ) in the presence and absence of light. Forty-five days after inoculation we assessed the percentage of callus induction, color, consistency, fresh and dry matter, total phenols, flavonoids, tannins contents, and chromatographic profile by HPLC-DAD method. Callus induction occurred only in medium with growth regulators. Maximal induction (100%) was found in medium containing 2,4-D combined with BAP in the presence and absence of light. We obtained friable and compact callus in yellow, green, and red. Culture media containing 4.52 M 2,4-D + 4.44 M BAP induced 100% of friable callus with higher fresh and dry weight in the absence of light. The callus produced higher amounts of total phenols and flavonoids than the initial explant.

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Elicitors as a Biotechnological Tool for In Vitro Production of Bioactive Phenolic Compounds

Castro

Coimbra

Crema

et al. 2021

Exploring Plant Cells for the Production of Compounds of Interest

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Establishment of callus from Pyrostegia venusta (Ker Gawl.) Miers and effect of abiotic stress on flavonoids and sterols accumulation

Cited by 14 publications

References 39 publications

Effect of Growth Regulators, Carbohydrates and Antioxidant Compounds on Biomass, Flavonoid Accumulation and Enzyme Activity in Callus Cultures of Rumex vesicarius L.

Effect of Growth Regulators, Carbohydrates and Antioxidant Compounds on Biomass, Flavonoid Accumulation and Enzyme Activity in Callus Cultures of Rumex vesicarius L.

Callus induction and bioactive phenolic compounds production fromByrsonima verbascifolia(L.) DC. (Malpighiaceae)

Elicitors as a Biotechnological Tool for In Vitro Production of Bioactive Phenolic Compounds

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