Elicitors as a Biotechnological Tool for In Vitro Production of Bioactive Phenolic Compounds

Effect of selected elicitors on phytochemical content and antioxidant activity of buckwheat (Fagopyrum esculentum) sprouts obtained from seeds cultivated using the hydroponics technology

“…The seeds were placed in trays containing cocopeat with neutral pH for sprouting (50 seeds for each treatment) with temperature and relative humidity maintained at 25 ± 2 °C and 60 ± 2% respectively in dark (Kim et al ., 2004). The seeds were sprayed with 6 mL distilled water for 5 days and then with elicitors (NaCl‐ 10, 50, 100, 150 m m ; Ascorbic acid‐ 0.5, 1, 1.5, 2 m m ; Citric acid‐ 10, 50, 100, 150 m m ; Mannitol‐ 10, 50, 100, 150 m m and Control‐ distilled water, 6 mL each) for the next 2 days after every 6 h. The concentrations of elicitors for optimization were selected after an extensive literature review (Natella et al ., 2016; Food Safety and Standards (Health Supplements, Nutraceuticals, Food for Special Dietary Use, Food for Special Medical Purpose, Functional Food and Novel Food) Regulations, 2016; U.S. Food and Drug Administration, Food Additive Status List, 2018; Liu et al ., 2019; Castro et al ., 2021). All the elicitors were freshly prepared in distilled water before application and sprouting took a total of 7 days to obtain suitable biomass for analysis.…”

Phenolic Acids and Amaryllidaceae Alkaloids Profiles in Leucojum aestivum L. In Vitro Plants Grown under Different Light Conditions

“…The biosynthesis of stress-inducible alkaloids is a natural adaptive response to abiotic and biotic stress factors [ 9 ]. In vitro stress caused by the action of elicitors is also often accompanied by increased biosynthesis of soluble sugars and phenolic compounds [ 26 , 27 , 28 ]. Soluble carbohydrates are synthesised, for example, in response to osmotic stress, protecting cellular members and improving survival under stress conditions [ 29 ].…”