Establishment of callus and cell suspension culture of Sophora alopecuroides Linn. for the production of oxymatrine

Abstract

Oxymatrine is one of the most important biologically active compounds and is present in Sophora alopecuroides L. The present investigation focuses on the development of an efficient tissue culture method to induce callus and cell suspension culture of S. alopecuroides by studying the effect of jasmonic acid and nitric oxide on cell suspension culture. Callus induction efficiency is high in axenic leaf explants grown in MS medium supplemented with 1.0 mg/L Kinetin (Kin), 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The cell suspension culture was developed using the same callus induction medium without agar. The maximum cell number and dry weight of suspension culture were obtained by the 9th day of incubation. The synthesis of oxymatrine is higher in jasmonic acid and nitric oxide (200 µMJA and 50 µMNO) combination (11.91 µg/g) when compared to the non-elicited control (8.3 µg/g) of callus.

Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

“…6A-C) gymnemic acid XVII production in G. sylvestre cell suspension cultures compared with respective control, however, this deacylgymnemic acid, gymnemagenin and gymnemic acid XVII yield was found to be 3.11, 1.78 and 1.18 fold lesser at SNP (40 µM) treated cultures compared with respective SNP 20 µM. In contrast to this study, Tsolmon et al (2020) in their investigation of Sophora alopecuroides cell suspension culture, reported that accumulation of oxymatrine content was 18.6 µg/g higher in suspension culture treated at higher 200 µM Jasmonic acid (JA) and 50 µM SNP combination. The ginsenoside accumulation was recorded highest at 200 µM of SNP treatment (Rahimi et al, 2016).…”

“…Recently, SNP has been reported as an elicitor involved in regulating several signalling responses (Siddiqui et al, 2017;Khezerluo et al, 2018;Hao et al, 2020;Kara et al, 2020). In previous studies, many researchers have reported that SNP treatments are very effective in shoot regeneration of Chrysanthemum cultivars (Arun et al, 2017), Canscora diffusa (Subiramani et al, 2019), Antirrhinum majus (Rezaei Zafarghandi and Rahmati Joneidabad 2020) Valeriana jatamansi (Pandey et al, 2020) and callus induction in Dioscorea opposita (Xu et al, 2009), Hyoscyamus niger (Samsampour et al, 2018) Ficus religiosa (Hesami et al, 2019) Canscora diffusa (Subiramani et al, 2019) and Valeriana jatamansi (Pandey et al, 2020), Furthermore, SNP play important roles in increasing metabolites accumulation (Khezerluo et al, 2018;Pradhan et al, 2020;Tsolmon et al, 2020).…”

Trichoderma and sodium nitroprusside elicitation improves vinblastine and vincristine yield by increasing TIA pathway genes expression in cell suspension of Catharanthus roseus

“…Nitric oxide activity in plant tissues and cells usually occurs in response to abiotic stresses, pathogen attacks, and fungal elicitor challenges. The most prominent role of nitric oxide is signaling and regulation of defense responses to stresses, which leads to increased production of secondary metabolites (Senthil 2020) and activation of enzymes and expression of genes related to their biosynthetic pathway (Ma et Trichoderma harzianum, Piriformospora indica and T. tomentosum 1% v/v in the periwinkle cell suspension was more effective than T. tomentosum. Gene expression began after 24 hours of treatment and expression of D4H and DAT genes after 48 hours of treatment had the highest gene expression of 1.2-fold and 0.7-fold, respectively.…”

Effects of 2,4-Dichlorophenoxyacetic Acid on The Induction of Callus from Cotyledon and Hypocotyl Explants of Butterfly Pea (Clitoria ternatea)

“…To date, efficient callus induction protocols for medicinal plants such as Astragalus missouriensis Nutt., Eysenhardtia polystachya and Sophora alopecuroides Linn. have been successfully developed, and the potential applications of in vitro callus culture have led to increased production of phytochemical compounds (Ionkoya, 2009;Bernabé-Antonio et al, 2017;Senthil, 2020). In vitro callus culture on C. ternatea, on the other hand, has yet to be investigated.…”