2008
DOI: 10.1128/jvi.02689-07
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Establishment of B-Cell Lines Latently Infected with Reactivation-Competent Murine Gammaherpesvirus 68 Provides Evidence for Viral Alteration of a DNA Damage-Signaling Cascade

Abstract: Gammaherpesvirus 68 (␥HV68, or MHV68) is a naturally occurring rodent pathogen that replicates to high titer in cell culture and is amenable to in vivo experimental evaluation of viral and host determinants of gammaherpesvirus disease. However, the inability of MHV68 to transform primary murine B cells in culture, the absence of a robust cell culture latency system, and the paucity of MHV68-positive tumor cell lines have limited an understanding of the molecular mechanisms by which MHV68 modulates the host cel… Show more

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Cited by 50 publications
(90 citation statements)
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“…Although the mechanisms are unclear, the DDR is utilized by gammaherpesviruses for viral replication (Barton et al, 2011;Sinclair et al, 2006;Weitzman et al, 2010). Several chemicals have been shown to induce the DDR from latently infected cells, including etoposide, camptothecin, cisplatin, and staurosporine (Feng et al, 2002;Forrest & Speck, 2008). These results suggest that the MHV-68 model will likely be sensitive to compounds with mechanisms of action that affect these pathways.…”
Section: Activating Factors and Evasion Of Host Defensesmentioning
confidence: 93%
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“…Although the mechanisms are unclear, the DDR is utilized by gammaherpesviruses for viral replication (Barton et al, 2011;Sinclair et al, 2006;Weitzman et al, 2010). Several chemicals have been shown to induce the DDR from latently infected cells, including etoposide, camptothecin, cisplatin, and staurosporine (Feng et al, 2002;Forrest & Speck, 2008). These results suggest that the MHV-68 model will likely be sensitive to compounds with mechanisms of action that affect these pathways.…”
Section: Activating Factors and Evasion Of Host Defensesmentioning
confidence: 93%
“…MHV-68 studies likewise discovered that histone acetylation was sufficient to re-activate the lytic cycle as a result of demethylation of the RTA promoter (Ben-Sasson & Klein, 1981;Chang & Liu, 2000;Chen et al, 2001;Countryman et al, 2008). In vitro, histone deacetylase inhibitors (such as butyric acid [NaB]) have also been shown to re-activate MHV-68 latently infected cell lines that might also have biological relevance in vivo (Forrest & Speck, 2008;Moser et al, 2005;Yang et al, 2009).…”
Section: Activating Factors and Evasion Of Host Defensesmentioning
confidence: 99%
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“…A20-HE-2 cells are A20 cells latently infected with MHV-68-EGFP (enhanced green fluorescent protein) virus. These cells were obtained from Dr Craig Forrest and Dr Samuel Speck (Emory University, Atlanta, GA) who had previously characterized them (see Forrest & Speck, 2008). All cells used in these studies were either provided directly from Forrest and Speck or were re-derived from these cells.…”
Section: Cellsmentioning
confidence: 99%