Establishment of age- and gender-specific pediatric reference intervals for liver function tests in healthy Han children

Li, Xin; Wang, Di; Yang, Chun; Zhou, Qi; Zhuoga, Suo-Lang; Wang, Liqiang; Yao, Hanxin; Zhang, Qin; Ai, Qing; Yang, Chenxi; Xu, Jiancheng

doi:10.1007/s12519-018-0126-x

Cited by 33 publications

(59 citation statements)

References 19 publications

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“…Clinical practice has demonstrated that adult RIs were not suitable to assess pediatric health status and diseases [12], For instance, Lv [13] et al stated that Chinese children's blood routine test RIs established by the direct method were not consistent with adults; Supriya [14] found acid α-glucose, glycosidase, acid α-galactosidase, acid β-glucocerebrosidase, acid sphingomyelinase, and galactocerebrosidase activity were different significantly among age groups in large-scale healthy Indian population; CAL-IPER (Canadian Laboratory Initiative on Pediatric Reference Intervals) and many scholars [15,16] confirmed that transferrin saturation increased slightly with age. It was proved in our previous experiment that pediatric liver function indicators were quite different from adults [17]. All these indicate that it is quite essential to establish appropriate and practical pediatric RIs.…”

Section: Introductionmentioning

confidence: 84%

“…e VITROS 5600 Integrated System underwent regular maintenance, function checks, calibration, quality control, and procedure according to the manufacturer's instructions. Analytical performance of the assays, such as precision, accuracy, analytical measurement range, and clinical reportable range, was monitored [17] and is shown in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…Now, pediatric RIs of clinical laboratories in China vary in source, such as reagent instructions, textbooks, literature, and self-information databases. Data are outdated and mixed, and credibility and accuracy are doubtful [17]. We intend to establish feasible RIs of CK, CKMB, AST, and LDH activity in Chinese children and adolescents by recruiting a large number of healthy children in Jilin Province and promote the development of pediatric RIs project.…”

Section: Introductionmentioning

confidence: 99%

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Age- and Sex-Specific Reference Intervals for Myocardial Enzyme Activity in Healthy Chinese Han Population Aged 1∼<18 years

Guo

Zhou

Jia

et al. 2019

BioMed Research International

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Age-and sex-specific reference intervals (RIs) for myocardial enzyme activity of children and adolescents are not available in China. Our study aimed to establish age-and gender-related RIs for AST, LDH, CK, and CKMB activity in healthy Chinese Han population aged 1∼<18 years. Healthy Han children and adolescents (n � 6322, 1∼<18 years old) were assessed from completed questionnaires and defined criteria from communities and schools in 5 administrative districts of Jilin Province from September 2017 to December 2018. Measurements of AST, LDH, CK, and CKMB activity were performed on the VITROS 5600 Integrated System. Percentiles of enzyme activity were completed by LMS. RIs were established by Medcalc according to the EP28-A3c guidelines issued by the Clinical and Laboratory Standards Institute. AST declined rapidly during 1∼<6 years and had been subsided during 11∼<18 years, though LDH decreased at a steady rate. CK activity stabilized while CKMB showed a downward trend. Sex differences started after age 12 when males presented higher results. ere were significant differences comparing with domestic and other countries' experiments which applied similar methodologies. Enzymes were associated with age and sex, while age had greater impact. We established age-and sex-specific RIs of serum AST, LDH, CK, and CKMB activities for Chinese children and adolescents using the VITROS 5600 Integrated System for the first time. ese data will lay the groundwork for the next horizon in pediatric RIs as well as improve test result interpretation for pediatric illness.

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Section: Introductionmentioning

confidence: 84%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Age- and Sex-Specific Reference Intervals for Myocardial Enzyme Activity in Healthy Chinese Han Population Aged 1∼<18 years

Guo

Zhou

Jia

et al. 2019

BioMed Research International

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“…Then according to Harris‐Boyd's method, the z values with the standard deviation and a calculated z for two groups to determine whether each group is sufficient different statistically to its own grouping. If the results of Harris‐Boyd's test did not demonstrate partition, the subgroup should be combined . Together with that, analysis of difference between sex and age was conducted using one‐way analysis of variance, the Mann‐Whitney U test, or the Kruskal‐Wallis test, as appropriate.…”

Section: Methodsmentioning

confidence: 99%

A preliminary study for the establishment of a reference interval for vitamin B12 in China after performance verification of a second‐generation ECLIA kit

Jiang

Men

Wen

et al. 2020

Clinical Laboratory Analysis

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Background:The second-generation electrochemiluminescence immunoassay (ECLIA) kit of vitamin B12 is widely used in clinical laboratories, and the establishment of a reference interval (RI) is essential to provide the basis for clinical monitoring. The purpose of this study was to establish a laboratory RI for vitamin B12 in China and at the same time verify the method performance of the second-generation kit. Methods:The verification of the method performance was conducted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Based on these guidelines, a total of 580 serum samples were collected, and 391 serum samples were used for the establishment of the RI according to CLSI guidelines. The subjects were grouped by sex and age. The age groups were as follows: 21-40, 41-60, and 61-80 years. The RI was defined by nonparametric 2.5th and 97.5th percentile intervals. Results:The performance of the second-generation kit of vitamin B12 from the Roche Cobas E602 system was in compliance with laboratory requirements. Serum vitamin B12 levels conformed to a non-Gaussian distribution. Harris-Boyd's test did not indicate partitioning for different age and gender group. Besides, there was no significant difference between different age groups (P = .07) and gender groups (P = .2002). The RI for healthy Chinese adults (aged 21-80 years) calculated by the nonparametric method was 250.8-957.1 pg/mL. Conclusions:The reference range of vitamin B12 was established, which provided a theoretical basis for the clinical application and monitoring of vitamin B12 detection. K E Y W O R D SChinese, electrochemiluminescent, performance verification, reference interval, Vitamin B12

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“…Reference interval (RI) is currently a hot spot in laboratory medicine. It is defined as the range between 2.5th percentile and 97.5th percentile of a certain indicator in healthy population [1]. As an important part of clinical laboratory and modern medicine, RI could provide valuable information for patients about diagnosis, progression, treatment, and outcome [2].…”

Section: Introductionmentioning

confidence: 99%

Division of Myocardial Enzyme Reference Intervals in Population Aged 1 to <18 Years Old Based on Fisher’s Optimal Segmentation Method

Guo

Zhou

Jia

et al. 2020

Computational and Mathematical Methods in Medicine

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Background. Reference interval (RI) research is to make it a concise, effective, and practical diagnostic tool. This study aimed to establish sex- and age-specific RI for myocardial enzyme activity in population aged 1–<18 years old in Changchun, China. Methods. Healthy subjects (n = 6,322, 1–<18 years old) were recruited from communities and schools. Aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), and creatine kinase isoenzyme (CKMB) were measured using an automatic biochemical analyzer. Fisher’s optimal segmentation method was used to partition by including percentiles as impact factors, aiming at minimizing the sum of the squares of the total dispersion into groups as splitting sequence of ordered data. Results. AST decreased gradually and was partitioned as 1, 2∼<10 and 10∼<18 years old. LDH presented disparate descending rate among 1∼<4, 4∼<12, and 12∼<18 years old. CK stood quite stable with the same RI in all ages. CKMB began to differ at 6 years of age sexually and then remained stable during 6∼<14 years old for male while it continued to decline in female. Cardiac development was partitioned as 1∼<6, 6∼<13, and 13∼<18 years old using multiple percentiles from massive data that reflect characteristics of totality as impact factors. Conclusions. Fisher’s optimal segmentation method excelled for multidimensionality, continuity, and loop calculating as dealing with RIs for myocardial enzymes activity and cardiac development process despite limitations. In future, impact of partition on the overall interval should be delved into.

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Establishment of age- and gender-specific pediatric reference intervals for liver function tests in healthy Han children

Cited by 33 publications

References 19 publications

Age- and Sex-Specific Reference Intervals for Myocardial Enzyme Activity in Healthy Chinese Han Population Aged 1∼<18 years

Age- and Sex-Specific Reference Intervals for Myocardial Enzyme Activity in Healthy Chinese Han Population Aged 1∼<18 years

A preliminary study for the establishment of a reference interval for vitamin B12 in China after performance verification of a second‐generation ECLIA kit

Division of Myocardial Enzyme Reference Intervals in Population Aged 1 to <18 Years Old Based on Fisher’s Optimal Segmentation Method

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