Age- and Sex-Specific Reference Intervals for Myocardial Enzyme Activity in Healthy Chinese Han Population Aged 1∼<18 years

Abstract

Age-and sex-specific reference intervals (RIs) for myocardial enzyme activity of children and adolescents are not available in China. Our study aimed to establish age-and gender-related RIs for AST, LDH, CK, and CKMB activity in healthy Chinese Han population aged 1∼<18 years. Healthy Han children and adolescents (n � 6322, 1∼<18 years old) were assessed from completed questionnaires and defined criteria from communities and schools in 5 administrative districts of Jilin Province from September 2017 to December 2018. Measurements of AST, LDH, CK, and CKMB activity were performed on the VITROS 5600 Integrated System. Percentiles of enzyme activity were completed by LMS. RIs were established by Medcalc according to the EP28-A3c guidelines issued by the Clinical and Laboratory Standards Institute. AST declined rapidly during 1∼<6 years and had been subsided during 11∼<18 years, though LDH decreased at a steady rate. CK activity stabilized while CKMB showed a downward trend. Sex differences started after age 12 when males presented higher results. ere were significant differences comparing with domestic and other countries' experiments which applied similar methodologies. Enzymes were associated with age and sex, while age had greater impact. We established age-and sex-specific RIs of serum AST, LDH, CK, and CKMB activities for Chinese children and adolescents using the VITROS 5600 Integrated System for the first time. ese data will lay the groundwork for the next horizon in pediatric RIs as well as improve test result interpretation for pediatric illness.

Age- and Sex-Specific Pediatric Reference Intervals of Serum Electrolytes in Jilin Province of China Using the A Priori Approach

“…In fact, our research group has conducted some studies in the early stage, and the results confirm that age, sex, and statistical methods have a certain influence on the reference interval. 3 , 4 Therefore, establishing accurate RIs for local laboratories is one of the most important factors for clinical diagnosis.…”

Reference Intervals for 26 Common Biochemical Analytes in Term Neonates in Jilin Province, China

“…Previous research has found that the differences in RIs of analytes are due to their complex patterns of biological sources of variations, such as region, race, diet, and more individual characteristic [1,2]. Data from the studies we have published con rms that establishing age-and sexappropriate local RIs is more accurate and reliable for providing clinical decision support, especially in pediatrics [3][4][5][6].…”

“…The samples were transported to the laboratory and centrifuged at 1200 ×g for 10 min within 1 h after collection. Specimens that were icteric, hemolyzed, or lipemic were removed prior to analysis, and then the activity or concentration of 26 biochemical analytes were determined on the VITROS 5600 Integrated Quali ed laboratory pro ciency and staff training were described in the research conducted in the same period [3,4]. Analytical procedures were performed according to the manufacturer's instructions and laboratory protocols, including regular maintenance, function checks, calibration, and internal quality control.…”

Reference intervals for 26 common biochemical analytes in term neonates in Jilin Province, China

“…3 Biochemical reference intervals in comparison with other studies. [ 1 ] represents the current study, [ 2 ] represents kit insert (F for female, M for male), [ 3 ] represents Choi et al, [ 4 ] represents Zhao et al, [ 5 ] represents Liu et al, [ 6 ] represents Higgins et al …”

“…Data from several studies suggest that age-and sex-specific RIs are more reliable to provide clinical decision support in the pediatric population [3][4][5]. Moreover, differences in analytical systems and statistical procedures may result in the differences between RIs reported in different studies [6][7][8][9]. Every aspect should be considered during the determination of accurate RIs.…”